辣蓼对大肠埃希菌性腹泻小鼠肠黏膜的修复作用

肖祝华; 罗晓韵; 程轩轩; 郑诗嘉; 杨全; 杨慧文

doi:10.13422/j.cnki.syfjx.20182220

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辣蓼对大肠埃希菌性腹泻小鼠肠黏膜的修复作用

药理 | 更新时间：2024-09-23

- 辣蓼对大肠埃希菌性腹泻小鼠肠黏膜的修复作用
- Effect of Polygoni Hydropiperis Herba on Intestinal Mucosal Repair in Diarrhea Mice Induced by
- 中国实验方剂学杂志 2018年24卷第22期页码：120-126
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省公益研究与能力建设专项项目（2014A020208133）
- DOI：10.13422/j.cnki.syfjx.20182220
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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肖祝华, 罗晓韵, 程轩轩, 等. 辣蓼对大肠埃希菌性腹泻小鼠肠黏膜的修复作用[J]. 中国实验方剂学杂志, 2018,24(22):120-126.

XIAO Zhu-hua, LUO Xiao-yun, CHENG Xuan-xuan, et al. Effect of Polygoni Hydropiperis Herba on Intestinal Mucosal Repair in Diarrhea Mice Induced by [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 120-126.
肖祝华, 罗晓韵, 程轩轩, 等. 辣蓼对大肠埃希菌性腹泻小鼠肠黏膜的修复作用[J]. 中国实验方剂学杂志, 2018,24(22):120-126. DOI： 10.13422/j.cnki.syfjx.20182220.

XIAO Zhu-hua, LUO Xiao-yun, CHENG Xuan-xuan, et al. Effect of Polygoni Hydropiperis Herba on Intestinal Mucosal Repair in Diarrhea Mice Induced by [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 120-126. DOI： 10.13422/j.cnki.syfjx.20182220.

摘要

目的：研究辣蓼对大肠埃希菌性腹泻小鼠的干预作用，并探讨其可能的作用机制。方法：选取160只昆明种小鼠随机分为正常组，模型组，小檗碱组，辣蓼低、中、高剂量组。除正常组外，其余各组采用腹腔注射致病性大肠埃希菌建立小鼠腹泻模型。辣蓼低、中、高剂量组分别灌胃2.5，5，7.5 g·kg-1辣蓼水提液，小檗碱组给予盐酸小檗碱0.025 g·kg-1，正常组及模型组以等体积生理盐水，每天给药2次。分别于成模后的第1，2，4，6天各组随机抽取5只动物，取十二指肠，光镜下观察病理形态学变化；采用酶联免疫吸附法（ELISA）测定肠组织中炎症因子白细胞介素-1β（interleukin-1β，IL-1β）和肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的水平；实时荧光定量聚合酶链式反应（Real-time PCR）检测肠组织中转化生长因子-β1（transforming growth factor-β1，TGF-β1），表皮生长因子受体（epidermal growth factor receptor，EGFR），Smad3及Smad7 mRNA表达水平。结果：病理学观察发现辣蓼干预后可以明显减轻腹泻小鼠十二指肠黏膜损伤；ELISA结果表明，与正常组比较，模型组小鼠肠黏膜TNF-α和IL-1β的含量明显增多（P<0.01）；与模型组比较，辣蓼中、高剂量组能降低小鼠肠黏膜TNF-α和IL-1β的含量（P<0.05，P<0.01）。Real-time PCR结果表明，与正常组比较，模型组TGF-β1，EGFR表达量均明显升高（P<0.05，P<0.01），Smad7 mRNA表达量明显降低（P<0.05，P<0.01），药物干预后，与模型组比较，各给药组可不同程度调节肠组织中TGF-β1，EGFR，Smad3及Smad7 mRNA表达量（P<0.05），且呈现一定的剂量依赖关系。结论：辣蓼对大肠埃希菌性腹泻有良好的治疗作用，其作用机制可能与减少肠组织炎症因子释放、改善肠道黏膜屏障、干预TGF-β/Smads信号转导有关。

Abstract

Objective:To study the effect of Polygoni Hydropiperis Herba on diarrhea mice induced by Escherichia coli

in order to explore its possible mechanism. Method:A total of 160 KM mice were randomly divided into blank group

model group

berberine group and drug groups (high-dose

medium-dose and low-dose Polygoni Hydropiperis Herba). All of the other groups

except for the blank group

were injected intraperitoneally with E. coli. The blank group and the model group were given normal saline intragastrically

drug groups were administrated with Polygoni Hydropiperis Herba extract at the dose of 2.5

7.5 g·kg-1 respectively

and the berberine group was given 0.025 g·kg-1 of berberine.On the 1st

2nd

4th and 6th days after intraperitoneal injection with E. coli

5 mice were randomly selected from each group. Their duodenum histopathological features were observed under optical microscope. The content of interleukin-1β (IL-1β) and tumor necrosis factor-α(TNF-α) was determined by enzyme linked immunosorbent assay (ELISA)

the mRNA expression levels of transforming growth factor-β1 (TGF-β1)

and epidermal growth factor receptor (EGFR) Smad3 and Smad7 were detected by real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). Result:The duodenal intestinal mucosal injury in mice could be significantly alleviated by Polygoni Hydropiperis Herba. ELISA results showed that the content of TNF-α and IL-1β in the model group significantly increased compared with the blank group(P<0.01). The content of TNF-α and IL-1β in the Polygoni Hydropiperis Herba groups (at dose of 5 and 7.5 g·kg-1) was reduced obviously compared with the model group (P<0.05

P<0.01). Real-time PCR results showed that the mRNA expressions of TGF-β1

EGFR in the model group significantly increased (P<0.05

P<0.01)

and mRNA expression of Smad7 in the model group was significantly reduced (P<0.01) compared with the bland group. After administration

Polygoni Hydropiperis Herba and berberine can regulate mRNA expressions of TGF-β1

EGFR

Smad3 and Smad7 in duodenum of diarrhea mice(P<0.05)

in a dose-dependent manner. Conclusion:Extract of Polygoni Hydropiperis Herba has an obvious effect on diarrhea mice induced by E. coli

and the mechanism may be related to reducing the release of intestinal inflammatory cytokines

alleviating the intestinal mucosal barrier

and intervening TGF-β/Smads signal pathway.

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