Objective:To study the effect of total flavonoids from Puerariae Lobatae Radix on oxidative stress injury induced by H2O2 in human umbilical vein endothelial cells (HUVECs). Method:With HUVECs as the study object

the experiment was divided into blank group

model group

positive drug group (10 μmol·L-1

Edaravone) and total flavone from Pueraria Lobatae Radix groups (10

20

40

80

160 mg·L-1). Methyl thiazolyl tetrazolium(MTT) assay was used to detect the protective effect of total flavonoids of Puerariae Lobatae Radix on HUVECs. The contents of superoxide dismutase (SOD)

malondialdehyde (MDA) and nitric oxide (NO) were detected by spectrophotometry

and the content of endothelin-1(ET-1) in the cell supernatant was detected by enzyme linked immunosorbent assay (ELISA). The level of reactive oxygen(ROS) was detected by DCFH-DA

and Western blot was used to detect the expressions of endothelial nitric oxide synthase (eNOS) and vascular cell adhesion molecule-1 (VCAM-1). Result:Compared with normal group

model group showed a significantly lower cell survival rate

activity of SOD

level of NO and eROS protein expression (P<0.01)

and a significantly higher MDA

ET-1 level

cell ROS level and VCAM-1 protein expression (P<0.01). Compared with model group

total flavone from Pueraria Lobatae Radix (40

80

160 mg·L-1) could significantly increase the survival rate of HUVECs with oxidative stress injury (P<0.05

P<0.01)

and total flavone from Pueraria Lobatae Radix (20

40

80 mg·L-1) could significantly increase NO level and eNOS

and significantly decrease MDA and ET-1 levels

cell ROS level and VCAM-1 expression (P<0.05

P<0.01). Conclusion:Total flavonoids from Puerariae Lobatae Radix can protect human umbilical vein endothelial cells from oxidative stress injury induced by H2O2.The mechanism may be related to the regulation of oxidative stress-related factors of SOD

MDA

ET-1 and NO

and the expressions of endothelial cell function-related proteins eNOS and VCAM-1.