Objective:

2

To explore the difference of effect of raw and wine-processed products of Rhei Radix et Rhizoma extract on brain protection of rats with intracerebral hemorrhage(ICH) and the connotation of the theory of "wine processing could promote efficacy" of Rhei Radix et Rhizoma.

Method:

2

Wistar male rats were used to establish the ICH model

extracts of raw and wine-processed products of Rhei Radix et Rhizoma were used for intervention

and these rats were randomly divided into 6 groups(blank group

sham-operated group

model group

raw Rhei Radix et Rhizoma group

wine-processed Rhei Radix et Rhizoma group and Angong Niuhuangwan group). Then the apoptosis of hippocampal neurons was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay. The expression of zonula occludens-1(ZO-1) and vascular endothelial cadherin-2(VE-cadherin-2) in the hemorrhagic brain tissue was detected by immunohistochemical assay. The expression of reduced form of nicotinamide-adenine dinucleotide phosphate(NADPH) oxidase 2(NOX2) and tumor necrosis factor-

α

stimulated gene-6(TSG-6) in the hemorrhagic brain tissue was detected by Western blot. The content of glutathione(GSH) in the serum of ICH rats was detected by automatic enzyme-linked immunosorbent assay systems. Through these above methods

we investigated the differences between raw products and wine-processed products on brain protection of ICH rats.

Result:

2

Compared with the sham-operated group

the apoptosis index of the hippocampal neurons in the model group were increased significantly(

P

<

0.01)

the expression of ZO-1 was decreased significantly(

P

<

0.01)

the expression of NOX2 protein was increased significantly(

P

<

0.01)

the expression of TSG-6 protein was decreased significantly(

P

<

0.01)

the content of GSH was also decreased significantly(

P

<

0.01). Compared with the model group

raw and wine-processed products of Rhei Radix et Rhizoma could reduce the apoptosis index of hippocampal neurons(

P

<

0.01)

increase the expression of ZO-1(

P

<

0.01) and VE-cadherin-2 protein(

P

<

0.05)

decrease the expression of NOX2(

P

<

0.05

P

<

0.01)

increase the expression of TSG-6 protein(

P

<

0.05) and increase the content of GSH(

P

<

0.01) in ICH rats.

Conclusion:

2

The raw and wine-processed products of Rhei Radix et Rhizoma all have brain protective effect on ICH rat model

but the protective effect of wine-processed products is slightly better than that of raw products. The result of this study provides experimental basis for exploring the theory of "wine processing could promote efficacy" of this herb.