Objective:To explore the method of multi-index quality control for Aolanti Weikang tablets

and establish its HPLC characteristic spectrum and determine the contents of main flavonoid glycosides in Aolanti Weikang tablets. Method:Neoeriocitrin

narirutin

naringin

hesperidin and neohesperidin were selected as index components

HPLC analysis was applied on an YMC-Pack ODS-A column(4.6 mm×250 mm

5 μm)

the mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution(18:82)

the detection wavelength was set at 283 nm

the flow rate was 1.0 mL·min-1

and the column temperature was 30℃. Result:HPLC characteristic spectrum was established

and five characteristic peaks in ten batches of Aolanti Weikang tablets were simultaneously determined.Five components(neoeriocitrin

narirutin

naringin

hesperidin and neohesperidin) all showed good linear relationship within the ranges of 0.266 5-10.66 mg·L-1(r=0.999 9)

1.055-42.20 mg·L-1(r=1.000 0)

7.070-282.8 mg·L-1(r=1.000 0)

0.797 8-31.91 mg·L-1(r=0.999 9)

6.955-278.2 mg·L-1(r=1.000 0)

respectively.The average recoveries of these five components were 99.4%(RSD 1.0%)

100.5%(RSD 0.6%)

97.8%(RSD 0.9%)

96.7%(RSD 0.7%) and 98.3%(RSD 0.8%).The similarity of characteristic spectrum between Aolanti Weikang tablets and the total flavonoid glycosides extract of Aurantii Fructus Immaturus was 1.000. Conclusion:The proposed HPLC characteristic spectrum and determination method of five flavonoid glycosides can be used as the quality control method of Aolanti Weikang tablets with multitude indexes.