Objective: To establish a method for content identification of main chemical compositions in Laportea bulbifera extract by using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS). Method: The chromateographic separation was performed on Agilent Eclipse Plus C18 RRHD (2.1 mm×100 mm

1.8 μm) with 0.1% formic acid solution-0.1% formic acid acetonitrile solution as mobile phase for gradient elution. The flow rate was 0.25 mL·min-1; column temperature was set at 40℃ and injection volume was 1 μL. Electrospray ionization (ESI) was applied and operated in negative ion mode. By using Data Analysis software combined with retention time

exact relative molecular weight and reference data

the structures of the monitored chemical compositions were identified. Result: Thirteen chemical compounds were identified by molecule mass information

reference data and present standards

including 4 catechins

3 phenylpropanoids and 6 flavonoids. Among them

neochlorogenic acid

cryptochlorogenic acid

chlorogenic acid

rutin

isoquercitrin

luteoloside

kaempferol-3-O-rutinoside

astragalin and quercitrin were first found in L. bulbifera. Conclusion: The established detection method was convenient and accurate

providing an analytical approach for rapidly identifying and analyzing the chemical compounds of L. bulbifera extract

and laying a foundation for its quality control as well as further studies on clarifying the material basis of its efficacy.