Objective:

2

To elucidate the rationality of integrated processing technology of Leonuri Herba based on comparison of chemical constituents and pharmacological effect of Leonuri Herba between traditional and integrated processing technology.

Method:

2

The contents of stachydrine hydrochloride

leonurine hydrochloride

rutin

hyperoside and isoquercetin were used as indexes to compare the differences in the contents of chemical constituents between traditional and integration processing technology of Leonuri Herba.Effect of Leonuri Herba with different processing technology on auricular swelling induced by dimethylbenzene in mice were observed to compared the differences of their anti-inflammatory effect.And rat acute blood stasis model was used to compare the differences of Leonuri Herba with different processing technology on hemorheology and blood coagulation indexes.

Result:

2

Contents of stachydrine hydrochloride

leonurine hydrochloride

rutin

hyperoside and isoquercetin in products of integrated processing were 1.558%

0.168%

0.137%

0.113% and 0.078%

they were 1.482%

0.134%

0.125%

0.082% and 0.071% in products of traditional processing

respectively.Both of the processing methods could reduce the degree of swelling and the contents of tumor necrosis factor(TNF)-

α

interleukin(IL)-1

β

IL-6 in mouse serum.And both of the processing methods could reduce the whole blood viscosity

prolong the thrombin time(TT)

prothrombin time(PT)

activated partial thromboplastin time(APTT) and reduce the concentration of plasma fibrinogen(FIB) in acute blood stasis model rats.

Conclusion:

2

Compared with the traditional processing technology

the integrated processing technology is better in guaranteeing the quality of Leonuri Herba decoction pieces and reducing the production cost

which indicates that the integrated processing of Leonuri Herba is reasonable.