RS3型芭蕉芋抗性淀粉的减肥降脂作用及急性毒性分析

邱敏懿; 晋小雁; 张彩娟; 张驰; 王敏; 卢彦冰; 赵保胜; 张元; 王学勇

doi:10.13422/j.cnki.syfjx.20190130

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RS3型芭蕉芋抗性淀粉的减肥降脂作用及急性毒性分析

药理 | 更新时间：2021-02-09

- RS3型芭蕉芋抗性淀粉的减肥降脂作用及急性毒性分析
- Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity
- 中国实验方剂学杂志 2019年25卷第1期页码：149-156
- 作者机构：
  
  1.北京中医药大学　中药学院，　北京中医药研究院，北京　 100029
  2.北京联合大学　生物化学工程学院，北京　 100023
- 作者简介：
  
  邱敏懿，在读硕士，从事药品鉴定、民族药药效及物质基础研究，E-mail：qiuminyi123@163.com
  王学勇，博士，教授，博士生导师，从事药用植物与分子生药学及民族药新药研发，Tel：010-64287660，E-mail:wxyph.d@163.com
- 基金信息：
  
  中央本级重大增减支项目(2060302);国家自然科学基金项目(81773841)
- DOI：10.13422/j.cnki.syfjx.20190130
  中图分类号： R28;R285;R285.5
- 收稿日期：2018-07-28，
  
  网络出版日期：2018-10-17，
  
  纸质出版日期：2019-01-05
- 稿件说明：
移动端阅览
邱敏懿, 晋小雁, 张彩娟, 等. RS3型芭蕉芋抗性淀粉的减肥降脂作用及急性毒性分析[J]. 中国实验方剂学杂志, 2019,25(1):149-156.

Min-yi QIU, Xiao-yan JIN, Cai-juan ZHANG, et al. Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 149-156.
邱敏懿, 晋小雁, 张彩娟, 等. RS3型芭蕉芋抗性淀粉的减肥降脂作用及急性毒性分析[J]. 中国实验方剂学杂志, 2019,25(1):149-156. DOI： 10.13422/j.cnki.syfjx.20190130.

Min-yi QIU, Xiao-yan JIN, Cai-juan ZHANG, et al. Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 149-156. DOI： 10.13422/j.cnki.syfjx.20190130.

摘要

目的：

评价芭蕉芋RS3型抗性淀粉对肥胖型高脂血症小鼠的减肥降脂作用，并进行安全性评价。

方法：

KKAy小鼠高脂饲料喂养20周，建立肥胖型高脂血症模型，随机分为模型组，辛伐他汀组（4 mg·kg

－1

），RS3抗性淀粉高、低剂量组（2，1 g·kg

－1

），并以维持饲料喂养的小鼠为正常组。给药组分别灌胃相应药物，正常组及模型组给予等量去离子水。8周后，处死小鼠，检测血清中总胆固醇（TC），甘油三酯（TG），高密度脂蛋白胆固醇（HDL-C），低密度脂蛋白胆固醇（LDL-C）及天门冬氨酸氨基转移酶（AST），丙氨酸氨基转氨酶（ALT）的含量，精密称量脂肪质量，计算脂/体比、体脂率及Lee's指数，伊红-苏木素（HE）染色观察肝脏及脂肪组织的病理学变化；采用限量法评价RS3抗性淀粉的安全性，连续观察14 d，记录小鼠毒副反应情况。

结果：

RS3抗性淀粉高剂量能显著降低小鼠体质量、脂肪质量、体脂率、脂/体比、Lee's指数，以及血清中TC

LDL-C

AST

ALT水平（

0.05）。组织形态学检测显示，给予RS3抗性淀粉可以明显改善肝脏组织的脂肪病变情况，保肝作用明显，可以抑制脂肪细胞膨大，降低小鼠脂肪累积，以高剂量为优；急毒实验小鼠按36 g·kg

－1

剂量给药后，动物未出现中毒反应及死亡。

结论：

RS3型芭蕉芋抗性淀粉具有良好的减肥降脂作用，且以高剂量组为佳，最大给药量证明无毒副作用，临床常用剂量安全可靠。

Abstract

Objective:

To evaluate effect of

Canna edulis

type 3 resistant starch(RS3) on weight loss and lipid reduction in obese hyperlipidemia mice and acute toxicity in mice.

Method:

KKAy mice were fed with high-fat diet for 20 weeks to establish a hyperlipidemia model and then randomly divided into model group

positive group (4 mg·kg

-1

)

high-dose resistant starch group and low-dose resistant starch group (2

1 g·kg

-1

). Mice in normal group were fed with standard diet. The medication groups received corresponding drugs by gavage. Normal group and high-fat model group were given equal volume of deionized water. After 8 weeks

mice were put to death. The levels of total cholesterol (TC)

triglyceride (TG)

high-density lipoprotein cholesterol (HDL-C)

low-density lipoprotein cholesterol (LDL-C)

aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum of mice were measured

and weigh fat mass

fat/body ratio

body fat rate and Lee's index were calculated accurately. The pathological changes of liver and adipose tissue were observed byhematoxylin-eosin (HE). The acute toxicity of RS3 to mice was evaluated by limit test. The mice were continuously observed for 14 days

and the toxicity of mice was recorded.

Result:

The indicators of high-dose RS3 group were significantly reduced

such as body weight

fat mass

body fat rate

fat/body ratio

Lee's index

and serum TC

LDL-C

AST

ALT levels(

0.05). Histomorphometric examination showed that the administration of RS3 starch could significantly improve the fatty lesions of liver tissue

and the liver-protecting effect was obvious

which could still inhibit the expansion of fat cells and reduce the accumulation of fat in mice.Among them

the high-dose group wsa better; After the maximum dose of 36 g·kg

-1

was administered

no toxic reaction and death occurred in the animals.

Conclusion:

RS3-type

Canna Edulis

Resistant Starch has a good effect in reducing body weight and serum lipid

with a better effect in the high-dose group and no toxicity. And the commonly used clinical dose is safe and reliable.

关键词

Keywords

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