风湿祛痛胶囊对大鼠胶原诱导性关节炎的影响

王靖霞; 刘春芳; 何莲花; 孙丛丛; 李逸群; 孟昊业; 彭江; 荆宇; 苗艳东; 林娜

doi:10.13422/j.cnki.syfjx.20190228

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风湿祛痛胶囊对大鼠胶原诱导性关节炎的影响

药理 | 更新时间：2021-02-09

- 风湿祛痛胶囊对大鼠胶原诱导性关节炎的影响
- Effect of Fengshi Qutong Capsule on Collagen-induced Arthritis of Rats
- 中国实验方剂学杂志 2019年25卷第2期页码：89-95
- 作者机构：
  
  1.承德医学院，河北　承德　 067000；
  2.中国中医科学院　中药研究所，北京　 100700；
  3.中国人民解放军总医院　骨科研究所，北京　 100853；
  4.通化金马药业集团股份有限公司，北京　 100028
- 作者简介：
  
  王靖霞，在读硕士，从事中药药理研究，E-mail：1476162527@qq.com
  *林娜，研究员，博士生导师，从事抗炎中药药理研究，E-mail：linna888@163.com
- 基金信息：
  
  中国中医科学院中药研究所技术研发项目(20171011)
- DOI：10.13422/j.cnki.syfjx.20190228
  中图分类号： R289; R289.5; R684.3
- 收稿日期：2018-09-04，
  
  网络出版日期：2018-11-02，
  
  纸质出版日期：2019-01-20
- 稿件说明：
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王靖霞, 刘春芳, 何莲花, 等. 风湿祛痛胶囊对大鼠胶原诱导性关节炎的影响[J]. 中国实验方剂学杂志, 2019,25(2):89-95.

Jing-xia WANG, Chun-fang LIU, Lian-hua HE, et al. Effect of Fengshi Qutong Capsule on Collagen-induced Arthritis of Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 89-95.
王靖霞, 刘春芳, 何莲花, 等. 风湿祛痛胶囊对大鼠胶原诱导性关节炎的影响[J]. 中国实验方剂学杂志, 2019,25(2):89-95. DOI： 10.13422/j.cnki.syfjx.20190228.

Jing-xia WANG, Chun-fang LIU, Lian-hua HE, et al. Effect of Fengshi Qutong Capsule on Collagen-induced Arthritis of Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 89-95. DOI： 10.13422/j.cnki.syfjx.20190228.

摘要

目的：

观察风湿祛痛胶囊对大鼠胶原诱导性关节炎(CIA)的干预作用。

方法：

SD大鼠随机分为正常组、模型组、风湿祛痛胶囊低、中、高剂量组(0.25，0.5，1 g·kg

－1

·d

－1

)，对照药甲氨蝶呤组(0.2 mg·kg

－1

·d

－1

)，除正常组外，其他各组以Ⅱ型胶原和不完全弗氏佐剂免疫大鼠建立CIA模型，首次免疫后第1天给药组开始灌胃给药，每天1次，共给药19 d；首次免疫第8天开始观察大鼠关节红肿和畸形症状、评价关节炎临床积分和发病率，第19天取材后，炎症关节进行微计算机断层扫描技术检测和骨计量学分析，同时进行炎症关节组织病理学检查并从滑膜炎症、血管翳、软骨侵蚀和骨破坏等方面进行半定量评分，酶联免疫吸附测定法检测血清白细胞介素-1

(IL-1

)，肿瘤坏死因子-

(TNF-

)，血管内皮生长因子(VEGF)，基质金属蛋白酶-3(MMP-3)和核转录因子-

B受体活化因子配基(RANKL)含量。

结果：

风湿祛痛胶囊能剂量依赖地改善CIA大鼠炎症关节红、肿和畸形症状；与正常组比较，模型组大鼠炎症关节红、肿和畸形明显临床积分和发病率、关节滑膜炎症、血管翳、软骨侵蚀和骨破坏的病理评分显著升高(

0.01)，炎症关节骨密度(BMD)，骨体积分数(BV/TV)，骨小梁厚度(Tb.Th)，骨小梁数量(Tb.N)显著降低(

0.01)，骨表面积体积比(BS/BV)和骨小梁分离度(Tb.Sp)显著升高(

0.01)，血清IL-1

，TNF-

，VEGF，MMP3和RANKL的含量显著升高(

0.01)；与模型组相比，各风湿祛痛胶囊组红肿、畸形症状明显改善，临床积分和发病率、关节滑膜炎症、血管翳、软骨侵蚀和骨破坏的病理评分均明显降低(

0.05，

0.01)，BMD，BV/TV，Tb.Th，Tb.N明显升高(

0.05，

0.01)，BS/BV和Tb.Sp明显降低(

0.05，

0.01)，血清IL-1

，TNF-

，VEGF，MMP-3和RANKL的含量明显降低(

0.05，

0.01)，且甲氨蝶呤组与高剂量组的作用相近。

结论：

风湿祛痛胶囊能有效缓解CIA的临床症状和病情，降低发病率，减轻关节的组织病理学和影像学严重度，同时抑制血清中的促炎细胞因子含量。

Abstract

Objective:

To observe the intervention effect of Fengshi Qutong capsule on collagen-induced arthritis (CIA) in rats.

Method:

SD rats were randomly divided into normal group

model group

low

medium and high-dose Fengshi Qutong capsule groups (0.25

0.5

1 g·kg

-1

·d

-1

)

and methotrexate group(0.2 mg·kg

-1

·d

-1

). Except for normal group

the other groups were immunized with type Ⅱ collagen and incomplete Freund's adjuvant to establish a CIA model. On the 1

day after the first immunization

the administration group was given intragastric administration

once a day

for 19 days; on the 8

day after the first immunization

the symptoms of joint swelling and malformation of the rats were observed

and the clinical scores and incidence of arthritis were evaluated. On the 19

day

micro-computed tomography and bone metrology were performed

and histopathological examination of inflammatory joints was performed

andsynovial inflammation

vasospasm

cartilage erosion and bone destruction by pathological severity scores

serum interleukin-1

(IL-1

)

tumor necrosis factor-

(TNF-

)

vascular endothelial growth factor (VEGF)

matrix metalloproteinase-3 (MMP-3) and receptor activator of nuclear factor kappa B ligand(RANKL)were detected by enzyme linked immunosorbent assay.

Result:

Fengshi Qutong capsule could improve the symptoms of inflammatory joint redness

swelling and deformity in CIA rats in a dose-dependent manner. Compared with normal group

clinical score and incidence

joint synovial inflammation

vasospasm

cartilage erosion and pathological score of bone destruction in joint group were significantly increased (

0.01)

bone mineral density (BMD)

bone volume/total volume (BV/TV)

trabecular thickness (Tb.Th)

trabecularnumber (Tb.N) were significantly decreased (

0.01)

bone surface area/bone volume (BS/BV) and trabecular separation (Tb.Sp) were significantly elevated (

0.01)

and levels of IL-1

TNF-

VEGF

MMP-3 and RANKL in serum were increased (

0.01); compared with model group

clinical scores and incidence

joint synovial inflammation

vasospasm

cartilage erosion and bone destruction of each drug groupwere significantly lower (

0.05

0.01)

BMD

BV/TV

Tb.Th

Tb.N were significantly elevated (

0.05

0.01)

BS/BV and Tb.Sp were significantly decreased (

0.05

0.01)

and levels of serum IL-1

TNF-

VEGF

MMP-3 and RANKL were significantly decreased (

0.05

0.01). And the control drug methotrexate had similar effects to the high-dose group.

Conclusion:

Fengshi Qutong capsule can effectively alleviate the clinical symptoms and conditions of experimental rheumatoid arthritis in rats

reduce the incidence

and relieve the histopathology and imaging severity

while inhibiting the inflammatory cytokines.

关键词

Keywords

references

郭炜，刘春芳，林娜．类风湿性关节炎滑膜血管新生与中医药 [J]. 中国实验方剂学杂志， 2012 , 18 ( 10 ): 308 - 312 .

张春华．甲氨蝶呤与来氟米特治疗类风湿性关节炎的临床疗效和安全性研究 [J]. 重庆医学， 2015 , 44 ( 4 ): 511 - 513 .

姚晖．风湿祛痛胶囊结合穴位注射治疗类风湿性关节炎的临床研究 [J]. 世界中医药， 2016 , 11 ( 1 ): 52 - 54 .

孙璠璠，李萍，朱志杰，等．风湿祛痛胶囊对大鼠佐剂性关节炎的治疗作用及机制 [J]. 世界中医药， 2015 , 10 ( 12 ): 1918 - 1921,1925 .

Bevaart L , Vervoordeldonk M J , Tak P P. Evaluation of therapeutic targets in animal models of arthritis: how does it relate to rheumatoid arthritis？ [J]. Arthritis Rheum , 2010 , 62 ( 8 ): 2192 - 2205 .

LIU C , YANG Y , SUN D , et al . Total saponin from Anemone flaccida Fr.schmidt prevents bone destruction in experimental rheumatoid arthritis via inhibiting osteoclastogenesis [J]. Rejuvenation Res , 2015 , 18 ( 6 ): 528 - 542 .

Remmers E F , Joe B , Griffiths M M , et al . Modulation of multiple experimental arthritis models by collagen-inducedarthritis quantitative trait loci isolated in congenic rat lines: different effects of non-major histocompatibility complex quantitative trait loci in males and females [J]. Arthritis Rheum , 2002 , 46 ( 8 ): 2225 - 2234 .

ZHANG Y , BAI M , ZHANG B , et al . Uncovering pharmacological mechanisms of wu-tou decoction acting on rheumatoid arthritis through systems approaches: drug-target prediction, network analysis and experimental validation [J]. Sci Rep , 2015 , 5 : 9463 .

Bendele A , Mcabee T , Sennello G , et al . Efficacy of sustained blood levels of interleukin-1 receptor antagonist in animal models of arthritis: comparison of efficacy in animal models with human clinical data [J]. Arthritis Rheum , 1999 , 42 ( 3 ): 498 - 506 .

李培培，解国雄，宋珊珊，等．大鼠佐剂性关节炎模型表现特征及评价指标 [J]. 中国免疫学杂志， 2012 , 28 ( 5 ): 453 - 457 .

Hegen M , Keith J C , Collins M , et al . Utility of animal models for identification of potential therapeutics for rheumatoid arthritis [J]. Ann Rheum Dis , 2007 , 67 ( 11 ): 1505 - 1515 .

孙佳蕾，武平，陈白露，等．类风湿关节炎动物模型在中医研究中的应用 [J]. 现代中西医结合杂志， 2015 , 24 ( 4 ): 444 - 447 .

王志中，王勇，牟方祥，等．血小板、TNF- α 及IL-1 β 与活动期类风湿关节炎的相关性研究 [J]. 第三军医大学学报， 2011 , 33 ( 5 ): 469 - 472 .

任海霞，肖诚，李梢，等．类风湿性关节炎中的血管生成相关因子研究进展 [J]. 中国中医基础医学杂志， 2007 ( 4 ): 315 - 318 .

赵志玲．基质金属蛋白酶及其抑制剂与类风湿性关节炎的研究进展 [J]. 解放军药学学报， 2005 ( 1 ): 54 - 56 .

Okada Y , Nagase H , Harris E D. Matrix metalloproteinases1，2，and 3 from rheumatoid synovial cells are sufficient to destroy joints [J]. J Rheumatol , 1987 : 41 - 42 .

Wada T , Nakashima T , Hiroshi N , et al . Rankl-rank signaling in osteoclastogenesis and bone disease [J]. Trends Mol Med , 2006 , 12 ( 1 ): 17 - 25 .

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