Objective:

2

To compare

in vitro

dissolution behaviors of active ingredients(gastrodin

parishin A

p

-hydroxybenzyl alcohol

parishin B and parishin C) in Gastrodiae Rhizoma powder with different particle size.

Method:

2

In vitro

dissolution of Gastrodiae Rhizoma powder in different dissolution media(water

artificial gastric juice and artificial intestinal juice) were detected by stirring paddle method.Dissolution of these five components in Gastrodiae Rhizoma powder with different particle size was determined by HPLC

mobile phase was acetonitrile-0.1%phosphoric acid solution for gradient elution

column temperature was set at 40 ℃ and detection wavelength was 220 nm.

Result:

2

In water and artificial intestinal juice

the dissolution rates of five active components in three kinds of Gastrodiae Rhizoma ultrafine powders were higher than that of the fine powder and the finest powder; in artificial gastric juice

the dissolution rates of gastrodin and

p

-hydroxybenzyl alcohol in Gastrodiae Rhizoma ultrafine powders were higher than that of the other powders

and the dissolution rate of parishin A in Gastrodiae Rhizoma ultrafine powders was lower than that of the other powders.

Conclusion:

2

An appropriate degree of superfine grinding can promote the dissolution of active ingredients in Gastrodiae Rhizoma

but not as fine as possible.The dissolution medium has an obvious influence on the dissolution behaviors of active components

which provides a reference for screening optical particle size of Gastrodiae Rhizoma powder in clinical application.