Objective:

2

To prepare standard decoction of Chuanxiong Rhizoma and conduct its quality analysis.

Method:

2

According to the requirements of standard decoction

15 batches of standard decoction of Chuanxiong Rhizoma from three producing areas were prepared

the HPLC fingerprint was established and the quality analysis was carried out by cluster analysis; under common pattern of fingerprint

the simultaneous determination of four index components(ferulic acid

senkyunolide I

senkyunolide A and ligustilide) was established by HPLC.The transfer rates of main components

dry extract yield

pH value of samples were measured.

Result:

2

A total of 15 batches of standard decoctions of Chuanxiong Rhizoma were fingerprinted by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(edition of 2012A). Twenty-two common peaks were identified

and their similarities were all greater than 0.92

and peak 11

13

17

18

19 and 20 were identified qualitatively as ferulic acid

senkyunolide I

senkyunolide A

n

-butylphthalide

coniferyl ferulate and ligustilide

respectively.The quality overview of standard decoction of Chuanxiong Rhizoma from three producing areas could be distinguished through cluster analysis

which showed that there were differences in quality of Chuanxiong Rhizoma from different producing areas

but the quality was relatively stable in different batches of samples from the same origin.Under common pattern

there were four major components in 15 batches of standard decoction of Chuanxiong Rhizoma

including ferulic acid

senkyunolide I

senkyunolide A and ligustilide.Contents of senkyunolide A(0.176 3-0.249 8 g·L

-1

) and senkyunolide I (0.065 2-0.103 4 g·L

-1

) was high in the standard decoction

content of ligustilide(0.040 0-0.089 8 g·L

-1

) followed

and content of ferulic acid(0.022 0-0.042 3 g·L

-1

) was the lowest

transfer rates of the above four components were 6.63%-11.82%

33.32%-55.98%

1.26%-3.73%and 16.39%-33.05%

respectively.Dry extract yield of the standard decoction was 12.69%to 19.78%

and the pH was 4.54 to 4.82.

Conclusion:

2

This study establishes the fingerprint and multi-component determination methods of standard decoctions of Chuanxiong Rhizoma from various producing areas

which is suitable for quality control of this standard decoction.