Objective:

2

To establish the quality control methods for the standard decoction of Puerariae Thomsonii Radix.

Method:

2

According to the preparation principles for traditional Chinese medicine(TCM) standard decoction

13 batches of Puerariae Thomsonii Radix from different origins were analyzed under the chromatography conditions established in this study and verified with methodology. By referring to

Chinese Pharmacopoeia

of 2015

puerarin was used as a quantitative indicator to calculate the transfer rate. In this study

the structures of main chromatographic peaks were also identified to clarify the main chemical constituents in the standard decoction.

Result:

2

The 13 batches of medicinal herbs were identified as Puerariae Thomsonii Radix

with a recovery rate of 98.0%

and RSD of 1.1%

indicating that the method was accurate and reliable. The transfer rate ranged from 41.4%to 60.0%

and the extraction rate was within the range of 15.7%-34.3%. The corresponding fingerprints were prepared for 13 batches of the standard decoction

and their similarities were all greater than 90.0%. The chemical constituents from Puerariae Thomsonii Radix were identified by mass spectrometry analysis

including citric acid

4′-

O

-glucosyl puerarin/daidzein-4′

7-diglucoside

3′-hydroxy puerarin/genistein puerarin

puerarin apioside

daidzin puerossid A and daidzein

etc.

Conclusion:

2

The 13 batches of Puerariae Thomsonii Radix decoction in different origins had consistent properties with the basic properties of medicinal decoction pieces. The established method of quality evaluation can be used to systematically evaluate the standard decoction

providing reference for quality control of related decoction preparations of Puerariae Thomsonii Radix.