Objective:

2

To analyze and compare the difference of volatile components between

Trifolium pratense

and

T

.

repens

seeds.

Method:

2

The volatile components were extracted by the headspace solid phase microextraction (HS-SPME) method and analyzed by gas chromatography-mass spectrometry (GC-MS)

and the relative contents of the components were calculated by the area normalization method.

Result:

2

Fourty six compounds were identified in the seeds of

T

.

pratense

with a total peak area of 3.48×10

9

for the volatile components. The compounds with high content in this species were 1-octen-3-ol (30.24%)

1-hexanol (11.94%)

heptane

2

2

4

6

6-pentamethyl (11.00%)

nonanal (7.74%)

octanal (6.79%) and hexanoic acid (6.04%). 44 compounds were identified from the seeds of

T

.

repens

with a total peak area of 6.37×10

9

for the volatile components. The compounds with high content in this species were 1-octen-3-ol (42.98%)

heptadecane (14.47%)

3-octanol (9.42%)

n

-caproic acid vinyl ester (8.82%) and pyrimidine-4

6-diol

5-methyl (6.59%). 20 components were commonly shared in the seeds of

T

.

pratense

and

T

.

repens

but the content of each component was quite different. 1-octen-3-ol had the highest content in the common components.

T

.

pratense

seeds had 26 unique components

accounting for 54.85%of volatile components.

T

.

repens

seeds had 24 unique components

accounting for 46.79%of volatile components.

Conclusion:

2

The seeds of both

T

.

pratense

and

T

.

repens

have rich volatile components

but there are great difference in the variety

peak area and relative content of the volatile components between these two varieties