养正散结汤对人胃癌MKN-45细胞增殖、凋亡及ERK通路的影响

肖真真; 邓海霞; 陈更新; 邱超芬; 周奥强

doi:10.13422/j.cnki.syfjx.20190327

您当前的位置：

首页 >

文章列表页 >

养正散结汤对人胃癌MKN-45细胞增殖、凋亡及ERK通路的影响

药理 | 更新时间：2021-02-09

- 养正散结汤对人胃癌MKN-45细胞增殖、凋亡及ERK通路的影响
- Effect of Yangzheng Sanjie Decoction on Proliferation， Apoptosis and ERK Pathway of Human Gastric Cancer MKN-45 Cells
- 中国实验方剂学杂志 2019年25卷第3期页码：39-44
- 作者机构：
  
  广州中医药大学　第二附属医院，广州　 510120
- 作者简介：
  
  肖真真，硕士，从事中医药治疗消化疾病的基础研究，Tel: 020-81887233，E-mail: 768197690@qq.com
  *陈更新，博士，主任医师，从事中医药防治消化系统疾病的研究，Tel: 020-81887233，E-mail: gxchen@gzucm.edu.cn
- 基金信息：
  
  国家自然科学基金项目(81273735);广东省高水平大学建设项目(A1-AFD018171Z11081);广东省中医院中医药科学技术研究专项(YN2016ML06;YN2014ZH05);广东省科技计划项目(2013B021800169)
- DOI：10.13422/j.cnki.syfjx.20190327
  中图分类号： R22;R242;R2-031;R273;R285.5
- 收稿日期：2018-08-29，
  
  网络出版日期：2018-11-16，
  
  纸质出版日期：2019-02-05
- 稿件说明：
移动端阅览
肖真真, 邓海霞, 陈更新, 等. 养正散结汤对人胃癌MKN-45细胞增殖、凋亡及ERK通路的影响[J]. 中国实验方剂学杂志, 2019,25(3):39-44.

Zhen-zhen XIAO, Hai-xia DENG, Geng-xin CHEN, et al. Effect of Yangzheng Sanjie Decoction on Proliferation， Apoptosis and ERK Pathway of Human Gastric Cancer MKN-45 Cells[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(3): 39-44.
肖真真, 邓海霞, 陈更新, 等. 养正散结汤对人胃癌MKN-45细胞增殖、凋亡及ERK通路的影响[J]. 中国实验方剂学杂志, 2019,25(3):39-44. DOI： 10.13422/j.cnki.syfjx.20190327.

Zhen-zhen XIAO, Hai-xia DENG, Geng-xin CHEN, et al. Effect of Yangzheng Sanjie Decoction on Proliferation， Apoptosis and ERK Pathway of Human Gastric Cancer MKN-45 Cells[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(3): 39-44. DOI： 10.13422/j.cnki.syfjx.20190327.

摘要

目的:

探讨养正散结汤对人胃癌MKN-45细胞增殖、凋亡及细胞外信号调节激酶(extracellular signal-regulated kinase

ERK)通路的影响。

方法:

将养正散结汤组(0.5，1，1.5，2，2.5，3，3.5 g·L

-1

)作用于胃癌MKN-45细胞，设置空白组，分别孵育24，48，72 h后采用细胞增殖活性检测方法(CCK-8)检测细胞增殖情况；用养正散结汤组(0.4，0.8 g·L

-1

)处理细胞，运用平板克隆形成实验观察细胞的克隆形成能力；用4，8 g·L

-1

养正散结汤干预细胞，利用流式细胞仪检测细胞的凋亡情况；用2，4，8 g·L

-1

养正散结汤处理细胞，采用蛋白免疫印迹法(Western blot)检测ERK表达及其磷酸化水平。

结果:

与空白组比较，养正散结汤组能明显抑制MKN-45细胞的增殖，处理24，48 h后，养正散结汤从2 g·L

-1

开始，处理72 h后，从1.5 g·L

-1

开始，细胞存活率逐渐降低，呈明显浓度依赖性(

0.05，

0.01)。经不同质量浓度的养正散结汤干预48 h后，养正散结汤组细胞克隆形成率显著低于空白组(

0.01)，呈一定的量效关系，0.8 g·L

-1

养正散结汤干预后，MKN-45细胞集落基本无法形成；养正散结汤组细胞凋亡率明显高于空白组(

0.05，

0.01)；干预12 h后，与空白组比较，养正散结汤从4 g·L

-1

开始下调MKN-45细胞中ERK蛋白的磷酸化水平(

0.01)。

结论:

养正散结汤能抑制人胃癌细胞MKN-45的增殖，促进其凋亡，其作用机制可能与抑制ERK的磷酸化有关。

Abstract

Objective:

To investigate the effect of Yangzheng Sanjie decoction on proliferation

apoptosis and extracellular signal-regulated kinase (ERK) pathway of human gastric cancer MKN-45 cells.

Method:

Gastric cancer cell line MKN-45 was treated for 24

72 h with Yangzheng Sanjie decoction (0.5

1.5

2.5

3.5 g·L

-1

); cell proliferation was measured by cell counting kit-8 (CCK-8); cell colony forming ability was observed by the plate cloning experiment after intervention with Yangzheng Sanjie decoction (0.4

0.8 g·L

-1

); MKN-45 cells was treated with 4

8 g·L

-1

and then cell apoptosis was detected by flow cytometry; the expression of ERK and its phosphorylation level were detected by Western blot assay after treatment with 2

8 g·L

-1

Result:

Compared with the blank group

Yangzheng Sanjie decoction could significantly inhibit the proliferation of MKN-45 cells. After treatment for 24

48 h

Yangzheng Sanjie decoction started from 2 g·L

-1

and after treatment for 72 h

it started from 1.5 g·L

-1

the cell viability gradually decreased in a concentration-dependent manner (

0.05

0.01). After 48 hours of intervention with different concentrations of Yangzheng Sanjie decoction

the cell clone formation rate of Yangzheng Sanjie decoction was significantly lower than that of the blank group with a dose-response relationship (

0.01). After the intervention with Yangzheng Sanjie decoction at a concentration of 0.8 g·L

-1

cell colonies could not be formed; the apoptosis rate of Yangzheng Sanjie decoction was significantly higher than that of the blank group (

0.05

0.01); after 12 h of intervention

compared with the blank group

Yangzheng Sanjie decoction started from 4 g·L

-1

and the phosphorylation level of ERK protein in MKN-45 cells was down-regulated (

0.01).

Conclusion:

Yangzheng Sanjie decoction can inhibit the proliferation of human gastric cancer cell line MKN-45 and promote its apoptosis. The mechanism may be related to the inhibition of phosphorylation of ERK.

关键词

Keywords

references

CHEN W Q , SUN K X , ZHENG R S , et al . Cancer incidence and mortality in China, 2014 [J]. Chin J Cancer Res , 2018 , 30 ( 1 ): 1 - 12 .

赵杰，陈振栋 . ERK和JNK在胃癌中的表达 [J]. 黑龙江医药科学， 2018 , 41 ( 1 ): 35 - 38 .

王秋红，杜桂清，邓桂霞，等 . SASH1和p-ERK在胃腺癌中的表达及意义 [J]. 诊断病理学杂志， 2017 , 24 ( 6 ): 440 - 444 .

陈更新，牟涛，李合国 . 养正散结汤治疗慢性萎缩性胃炎癌前病变55例临床观察 [J]. 吉林中医药， 2007 , 27 ( 12 ): 17 - 18 .

DENG H X , YU Y Y , ZHOU A Q , et al . Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression [J]. World J Gastroenterol , 2017 , 23 ( 30 ): 5538 - 5548 .

邓海霞，杨乃坤，陈万群，等 . 养正散结汤对人胃癌癌前病变细胞增殖、凋亡及抑癌性miRNA表达的影响 [J]. 中药新药与临床药理， 2017 , 28 ( 4 ): 478 - 481 .

邓海霞，陈更新，周奥强，等 . 养正散结汤对裸鼠胃癌移植瘤生长及let-7a/c-Myc表达的影响 [J]. 中国中西医结合消化杂志， 2017 , 25 ( 10 ): 738 - 741,746 .

余娅娅，周奥强，肖真真，等 . 养正散结汤调控miR-7/EGFR表达抑制胃癌细胞增殖 [J]. 中药新药与临床药理， 2018 , 29 ( 2 ): 131 - 135 .

曹超，黄秋林 . 扶正抑瘤方联合新辅助化疗、腹腔镜辅助胃癌根治术治疗进展期胃癌痰瘀毒结证患者的疗效观察 [J]. 中国实验方剂学杂志， 2018 , 24 ( 8 ): 176 - 182 .

欧阳兆云，王鹏飞，史作田，等 . 中医中药治疗胃癌的研究进展 [J]. 世界最新医学信息文摘， 2017 , 17 ( 83 ): 80 .

李姜，徐力，李烜 . 中医药调节肿瘤细胞信号传导的研究进展 [J]. 江苏中医药， 2016 , 48 ( 4 ): 81 - 85 .

Wee P , WANG Z X. Epidermal growth factor receptor cell proliferation signaling pathways [J]. Cancers , 2017 , 9 ( 5 ): 52 .

Giacomo C , Laura G , Giuliana C , et al . Modulation of sensitivity to antitumor agents by targeting the MAPK survival pathway [J]. Curr Pharm Des , 2013 , 19 ( 5 ): 883 - 894 .

Burotto M , Chiou V L , Lee J M , et al . The MAPK pathway across different malignancies: a new perspective [J]. Cancer , 2014 , 120 ( 22 ): 3446 - 3456 .

Hubbard S R , Miller W T. Receptor tyrosine kinases: mechanisms of activation and signaling [J]. Curr Opin Cell Biol , 2007 , 19 ( 2 ): 117 - 123 .

GAO M , LIANG X J , ZHANG Z S , et al . Relationship between expression of EGFR in gastric cancer tissue and clinicopathological features [J]. Asian Pacific J Tropical Med , 2013 , 6 ( 4 ): 260 - 264 .

WANG Y Z , XIAO H J , WU H T , et al . G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways [J]. Oncol Rep , 2017 , 37 ( 4 ): 1998 - 2006 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

养阴化瘀解毒方通过PI3K/Akt信号通路调控低氧环境下结肠癌细胞的自噬和凋亡

蛇床子素对人肝内胆管癌HuCCT1细胞增殖与凋亡的影响及其机制

基于Akt/FoxO信号通路探讨熊果酸对结直肠癌细胞增殖和凋亡的影响

基于JAK2/STAT3信号通路探讨芪苓白头翁汤对弥漫大B细胞淋巴瘤细胞增殖和凋亡的影响

基于Wnt/β-catenin通路探讨加味黄芪甘草汤对非小细胞肺癌细胞增殖、凋亡、侵袭、迁移及上皮间质转化的影响