Objective:

2

The homogeneity regularity of " imprinting templates" of compound preparation of traditional Chinese medicine(TCM) was revealed

which provided the basis for effectively controlling the quality of compound preparation of TCM

by clarifying the dynamic changeability in number and content of volatile ingredients in different batches of Houttuyniae Herba.

Method:

2

The volatile oil of Houttuyniae Herba was extracted by steam distillation.The volatile components in 11 batches of Houttuyniae Herba were determined by GC-MS(electron ionization

ion source temperature of 230 ℃

detection range of

m

/

z

40-500) combined with literature and ChemicalBook database.The relative content of each component was calculated by peak area normalization method and analyzed by the total quantum statistical moments of fingerprint.

Result:

2

There were only 15 common ingredients in the volatile oil from 11 batches of Houttuyniae Herba

accounting for 3.35%of total number and 73.94%of total content

while the number of non-common ingredients was 433

accounting for 96.65%of total number.However

the RSD of total quantum first moment of GC-MS fingerprint was 6.8%

the RSD of total quantum second moment was 14.8%(RSD of standard deviation was 7.4%)

and the RSD of similarity of total quantum statistical moment was 3.8%.The type and content of volatile ingredients in Houttuyniae Herba were heterogeneous

but their chromatographic behavior of GC-MS fingerprint was uniform

the whole " imprinting templates" was similar.

Conclusion:

2

The total quantum statistical moment(similarity) of TCM fingerprint is applicable to quality control of dynamic Chinese medicines

which will play a breakthrough role in the study of homogeneity and stability of material reference and compound preparation for ancient classical formulas.