Objective:

2

To compare the effects between crude and processed Epimedii Folium on the rats of kidney-Yang deficiency and edema

in order to discuss the mechanism.

Method:

2

The rat model of kidney-Yang edema was duplicated with hydrocortisone and doxorubicin hydrochloride. At day 1 and day 8 of modeling

the rats were injected with doxorubicin hydrochloride (3.5mg·kg

-1

) via tail vein

while being intraperitoneally injected with hydrocortisone (3.75mg·kg

-1

·d

-1

) for 15days.After modeling

the rats were divided into model group

crude Epimedii Folium group(204.86mg·kg

-1

)and processed Epimedii Folium group(204.86mg·kg

-1

)

and a normal control group was set up additionally.Rats in each treatment group were given the corresponding drugs

and those in normal and model groups were given the same volume of normal saline by gavage for continuous 14days.At the end of the administration

24h urine was measured by rat metabolic cage method; 24h urinary protein was detected by ultraviolet spectrophotometry; the content of plasma albumin (ALB) and total serum protein (TP) were measured by an automatic biochemical analyzer; and the content of adenosine monophosphate was measured by enzyme-linked immunosorbent assay(ELISA). Cyclic adenosine monophosphate (cAMP)

cyclic guanosine monophosphate (cGMP)

triiodothyronine (T

3

)

thyroxine (T

4

)

serum creatinine (SCr)

serum urea(BUN)

estradiol (E

2

)

testosterone (T) indicator content were determined by enzyme-linked immunosorbent assay(ELISA). Kidney tissues were collected to make pathological section by htoxylin eosin(HE) staining.

Result:

2

Compared with the normal group

all the indicators of appearance

biochemistry and pathological section in the model group indicated kidney Yang deficiency edema in the rats (

P

<

0.05

P

<

0.01). Compared with the model group

both crude Epimedii Folium group and processed Epimedii Folium group showed a regulatory effect on indicators of appearance and pathological section kidney Yang deficiency edema; and in biochemical indicators

both crude Epimedii Folium group and processed Epimedii Folium group can regulate urine

urine protein

SCr

BUN

cAMP/cGMP

E

2

T indicators to varying degrees(

P

<

0.05

P

<

0.01). Crude Epimedii Folium group showed a significantly regulatory effect on urine

urine protein

SCr

BUN (

P

<

0.01); whereas processed Epimedii Folium group showed a significantly regulatory effect on cAMP/cGMP

E

2

T (

P

<

0.01). In addition

crude Epimedii Folium group can also regulate ALB

TP (

P

<

0.05

P

<

0.01)

while processed Epimedii Folium group had no regulatory effect on these two indicators; however

processed Epimedii Folium group could regulate T

3

T

4

and anal temperature (

P

<

0.05

P

<

0.01)

while crude Epimedii Folium group had no regulatory effect on these two indicators.

Conclusion:

2

Both crude Epimedii Folium group and processed Epimedii Folium group have certain effect in treating kidney Yang deficiency edema

and the possible mechanism is to alleviate glomerular podocyte injury induced by doxorubicin. Their emphasis in the treatment of kidney Yang deficiency edema is different. Crude Epimedii Folium has a cold property

and can treat kidney Yang deficiency edema by strengthening renal excretion function of rats with kidney Yang deficiency edema. Processed Epimedii Folium is processed with sheep oil to produce icariin and other substances

and its property changed from cold to warm

with a focus on alleviating the kidney Yang deficiency status of rats with kidney Yang deficiency edema.