芸薹子中含氮类化学成分分离鉴定

荆文光; 孙晓波; 兰青山; 刘安

doi:10.13422/j.cnki.syfjx.20190513

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芸薹子中含氮类化学成分分离鉴定

资源与质量评价 | 更新时间：2021-02-09

- 芸薹子中含氮类化学成分分离鉴定
- Nitrogenous Chemical Constituents from Brassica campestris Seeds
- 中国实验方剂学杂志 2019年25卷第10期页码：127-133
- 作者机构：
  
  1.中国医学科学院&北京协和医学院　药用植物研究所，北京　 100193；
  2.中国中药有限公司，北京　 102600；
  3.中国中医科学院　中药研究所，北京　 100700
- 作者简介：
  
  荆文光，博士后，从事中药药效物质基础研究，E-mail：jingwenguang163@163.com
  *刘安，研究员，从事中药化学和质量控制研究，E-mail：liuan_508@163.com
- 基金信息：
  
  中国中医科学院“十三五”重点领域研究专项(ZZ10-007);国家中药标准化项目(ZYBZH-Y-ZY-45)
- DOI：10.13422/j.cnki.syfjx.20190513
  中图分类号： R284.2; R289; R2-031
- 收稿日期：2018-07-17，
  
  网络出版日期：2018-11-21，
  
  纸质出版日期：2019-05-20
- 稿件说明：
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荆文光, 孙晓波, 兰青山, 等. 芸薹子中含氮类化学成分分离鉴定[J]. 中国实验方剂学杂志, 2019,25(10):127-133.

Wen-guang JING, Xiao-bo SUN, Qing-shan LAN, et al. Nitrogenous Chemical Constituents from Brassica campestris Seeds[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 127-133.
荆文光, 孙晓波, 兰青山, 等. 芸薹子中含氮类化学成分分离鉴定[J]. 中国实验方剂学杂志, 2019,25(10):127-133. DOI： 10.13422/j.cnki.syfjx.20190513.

Wen-guang JING, Xiao-bo SUN, Qing-shan LAN, et al. Nitrogenous Chemical Constituents from Brassica campestris Seeds[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 127-133. DOI： 10.13422/j.cnki.syfjx.20190513.

摘要

目的：

芸薹子中含氮类化学成分的分离与鉴定。

方法：

干燥并经石油醚脱脂后的芸薹子采用75%的乙醇回流提取，减压浓缩后所得浸膏用水悬浮后分别用石油醚、乙酸乙酯萃取为不同极性部位，水部位利用AB-8大孔吸附树脂粗分为30%，60%，95%乙醇极性部位，最后采用硅胶柱色谱，LH-20型羟丙基葡聚糖凝胶(Sephadex LH-20)柱色谱以及半制备HPLC等方法进行分离纯化，根据理化常数和核磁共振谱、质谱等光谱数据对所分离的化学成分进行结构鉴定。

结果：

从芸薹子75%乙醇提取物中共分离鉴定了13个含氮类化合物，分别鉴定为coixspirolactam C(1)，吲哚-3-乙腈(2)，2-

-glucopyranosylsulfanyl-1H-indole-3-acetonitrile(3)，烟酸(4)，腺苷(5)，吲哚-3-甲醛(6)，4-羟基-吲哚-3-甲醛(7)，6-羟基-吲哚-3-甲酸(8)，ethyl 2-oxo-1，2，3，4-tetrahydroquinoline-4-carboxylate(9)，5-羟基-4(1H)-喹唑酮(10)，4-羟基-2(1H)-喹唑酮(11)，2(1H)，4(3H)-二羰基-5-羟基喹唑酮(12)，5-烯丙基噁唑啉-2-酮(13)。

结论：

以上所有化合物均为首次从芸薹子植物中分离得到，其中化合物1，3和13为首次从芸薹属植物中分离得到。

Abstract

Objective:

To study nitrogenous chemical constituents from the seeds of

Brassica campestris

Method:

The aerial parts of nonfat seeds were extracted with 75% ethanol heat refluxing method

then suspended in H

O and separated with ligarine and ethyl acetate to obtain respective fractions.Chemical constituents were isolated and purified by various chromatographic methods and identified by physicochemical characters and spectroscopic analysis or comparison with standard compounds.

Result:

Thirteen nitrogenous chemical compounds were isolated and identified as coixspirolactam C (1)

indole-3-acetonitrile (2)

-glucopyranosylsulfanyl-1H-indole-3-acetonitrile (3)

nicotinic acid (4)

adenosine (5)

1H-indole-3-carbaldehyde (6)

4-hydroxy-1H-indole-3-carbaldehyde (7)

6-hydroxy-1H-indole-3-carboxylic acid (8)

ethyl 2-oxo-1

4-tetrahydroquinoline-4-carboxylate (9)

5-hydroxy-4(1H)-quinazolinone (10)

4-hydroxy-2-quinazolinone (11)

5-hydroxy-quinazoline-2(1H)

4(3H)-dione (12)

5-allyloxazolidin-2-one (13).

Conclusion:

All of the compounds were isolated from this plant for the first time

and compounds 1

3 and 13 were isolated from Brassica for the first time.

关键词

Keywords

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