胃肠安颗粒剂与汤剂抑制人胃癌裸鼠皮下移植瘤生长、淋巴结转移作用比较及对RUFY3，SNAI1，VEGF和EMT蛋白的影响

李佳; 陈伟霞; 李朝燕; 赵爱光

doi:10.13422/j.cnki.syfjx.20190522

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胃肠安颗粒剂与汤剂抑制人胃癌裸鼠皮下移植瘤生长、淋巴结转移作用比较及对RUFY3，SNAI1，VEGF和EMT蛋白的影响

药理 | 更新时间：2021-02-09

- 胃肠安颗粒剂与汤剂抑制人胃癌裸鼠皮下移植瘤生长、淋巴结转移作用比较及对RUFY3，SNAI1，VEGF和EMT蛋白的影响
- Inhibition Effect of Weichang'an Granule and Decoction on Tumor Growth and Lymph Node Metastasis by RUFY3,SNAI1,VEGF,EMT Related Proteins in Nude Mice with Subcutaneous Xenograft Tumor of Human Gastric Carcinoma MKN45
- 中国实验方剂学杂志 2019年25卷第5期页码：92-97
- 作者机构：
  
  1.上海中医药大学　附属龙华医院，上海　 200023；
  2.郑州市中医院，郑州　 450000
- 作者简介：
  
  李佳，博士，主治医师，从事中西医结合防治消化道肿瘤研究，E-mail: 317436689@qq.com
  赵爱光，博士，主任医师，从事中西医结合防治消化道肿瘤研究，Tel：021-64385700，E-mail: 2538312201@qq.com
- 基金信息：
  
  国家重点研发计划项目(2017YFC1700605);国家中医临床研究基地业务建设科研专项(JDZX2015068);国家“重大新药创制”科技重大专项(2017ZX09304001)
- DOI：10.13422/j.cnki.syfjx.20190522
  中图分类号： R22;R242;R2-031;R285.5;R273
- 收稿日期：2018-08-07，
  
  网络出版日期：2018-11-19，
  
  纸质出版日期：2019-03-05
- 稿件说明：
移动端阅览
李佳, 陈伟霞, 李朝燕, 等. 胃肠安颗粒剂与汤剂抑制人胃癌裸鼠皮下移植瘤生长、淋巴结转移作用比较及对RUFY3，SNAI1，VEGF和EMT蛋白的影响[J]. 中国实验方剂学杂志, 2019,25(5):92-97.

jia LI, Wei-xia CHEN, Zhao-yan LI, et al. Inhibition Effect of Weichang'an Granule and Decoction on Tumor Growth and Lymph Node Metastasis by RUFY3,SNAI1,VEGF,EMT Related Proteins in Nude Mice with Subcutaneous Xenograft Tumor of Human Gastric Carcinoma MKN45[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 92-97.
李佳, 陈伟霞, 李朝燕, 等. 胃肠安颗粒剂与汤剂抑制人胃癌裸鼠皮下移植瘤生长、淋巴结转移作用比较及对RUFY3，SNAI1，VEGF和EMT蛋白的影响[J]. 中国实验方剂学杂志, 2019,25(5):92-97. DOI： 10.13422/j.cnki.syfjx.20190522.

jia LI, Wei-xia CHEN, Zhao-yan LI, et al. Inhibition Effect of Weichang'an Granule and Decoction on Tumor Growth and Lymph Node Metastasis by RUFY3,SNAI1,VEGF,EMT Related Proteins in Nude Mice with Subcutaneous Xenograft Tumor of Human Gastric Carcinoma MKN45[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 92-97. DOI： 10.13422/j.cnki.syfjx.20190522.

摘要

目的：

观察胃肠安颗粒与汤剂对MKN45人胃癌裸鼠皮下移植瘤皮下移植瘤生长、淋巴结转移作用及对RUN和FYVE结构域蛋白3(RUFY3)，锌指转录因子1(SNAI1)，血管内皮生长因子(VEGF)和上皮细胞间质转化(EMT)蛋白的影响。

方法：

建立人胃癌MKN45细胞裸鼠皮下移植瘤模型，随机分为空白（生理盐水0.5mL/只）组，胃肠安颗粒（胃肠安颗粒3.54g·kg

－1

）组，胃肠安煎剂（生药35.49g·kg

－1

）组。观察各组裸鼠皮下移植瘤瘤重、腋窝淋巴结的体积，苏木素-伊红(HE)染色检测各组淋巴结的形态，免疫组化检测包括RUFY3，SNAI1，VEGF

E-钙黏蛋白(E-cadherin)，N-钙黏蛋白(N-cadherin)，波形蛋白(Vimentin)相关蛋白表达。

结果：

与空白组比较，胃肠安颗粒组和胃肠安煎剂组皮下移植瘤瘤重显著降低(

0.01)，腋窝淋巴结体积显著减小(

0.01)；HE染色显示淋巴结中均可见转移的肿瘤细胞；裸鼠胃癌组织RUFY3，SNAI1，VEGF

N-cadherin

Vimentin蛋白表达显著降低(

0.01)，E-cadherin蛋白表达显著增加(

0.01)。

结论：

胃肠安颗粒与胃肠安煎剂在抑制人胃癌皮下移植瘤生长和淋巴结转移方面具有相同疗效，可在一定程度上代替汤剂使用；胃肠安颗粒和胃肠安煎剂均能降低裸鼠皮下移植瘤中RUFY3，SNAI1，VEGF

N-cadherin

Vimentin蛋白表达，增加E-cadherin蛋白表达，提示胃肠安可能通过调节RUFY3，SNAI1，VEGF

EMT相关蛋白的表达而起到抑制肿瘤转移的作用。

Abstract

Objective:

To observe the effect of Weichang'an on the tumor growth and lymph node metastasis

and RUN and FYVE domain-containing protein 3(RUFY3)

Zinc finger protein 1(SNAI1)

vascular endothelial growth factor(VEGF)

epithelial-mesenchymal transition(EMT)-related proteins in nude mice with subcutaneous xenograft tumor human gastric carcinoma MKN45

so as to discuss the mechanism of Weichang'an on MKN45 human gastric metastasis.

Method:

The nude mice model of human gastric carcinoma MKN45 cells was established and randomly divided into normal saline group (0.5mL/mouse)

Weichang'an granule group (3.54g·kg

-1

) and Weichang'an decoction group (35.49g·kg

-1

). The tumor weight and volume of axillary lymph nodes in each group were observed. The morphology of lymph nodes in each group was detected by hematoxylin-eosin (HE) staining. The expressions of related proteins were detected by immunohistochemistry

including RUFY3

SNAI1

VEGF

E-cadherin

N-cadherin

Vimentin.

Result:

Compared with the normal saline group

the tumor weight and volume of axillary lymph node were decreased (

0.01). HE staining revealed metastatic tumor cells in all lymph nodes. The positive expression rates of RUFY3

SNAI1

VEGF

N-cadherin

Vimentin in the Weichang'an granule group and Weichang'an decoction group were lower than those in normal saline group (

0.01)

while the positive expression of E-cadherin increased significantly (

0.01).

Conclusion:

Both Weichang'an granule and Weichang'an decoction can inhibit the tumor growth and metastasis of axillary lymph nodes

obviously down-regulate the expressions of RUFY3

SNAI1

VEGF

N-cadherin

Vimentin and up-regulate the expression of E-cadherin in human gastric MKN45 subcutaneous transplanted tumor in nude mice. This suggested that Weichang'an may inhibit the tumor metastasis through regulation expressions of RUFY3

SNAI1

VEGF and EMT-related proteins.

关键词

Keywords

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