使药石菖蒲对定志小丸中人参皂苷类成分肠吸收的影响

汪鼎; 刘晓闯; 纪娟; 彭代银; 周安

doi:10.13422/j.cnki.syfjx.20190552

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使药石菖蒲对定志小丸中人参皂苷类成分肠吸收的影响

经典名方 | 更新时间：2021-02-09

- 使药石菖蒲对定志小丸中人参皂苷类成分肠吸收的影响
- Effect of Acori Tatarinowii Rhizoma on Intestinal Absorption of Ginsenosides in Dingzhi Xiaowan
- 中国实验方剂学杂志 2019年25卷第8期页码：7-13
- 作者机构：
  
  1.安徽中医药大学　科研实验中心，中药复方安徽省重点实验室，合肥　 230038；
  2.安徽中医药大学　第一附属医院，合肥　 230031
- 作者简介：
  
  汪鼎，在读硕士，从事药物分析研究，E-mail: 411653657@qq.com
  周安，博士，教授，从事中药分析及药效物质基础研究，Tel: 0551-65169752，E-mail: anzhou@ahtcm.edu.cn
- 基金信息：
  
  国家自然科学基金项目(81303245);安徽省高校省级科学研究重点项目(KJ2013A171;KJ2016A398);安徽中医药大学研究生科技创新基金项目(2016YZ06)
- DOI：10.13422/j.cnki.syfjx.20190552
  中图分类号： R22;R24;R28;C37;R94
- 收稿日期：2018-09-26，
  
  网络出版日期：2018-11-19，
  
  纸质出版日期：2019-04-20
- 稿件说明：
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汪鼎, 刘晓闯, 纪娟, 等. 使药石菖蒲对定志小丸中人参皂苷类成分肠吸收的影响[J]. 中国实验方剂学杂志, 2019,25(8):7-13.

Ding WANG, Xiao-chuang LIU, Juan JI, et al. Effect of Acori Tatarinowii Rhizoma on Intestinal Absorption of Ginsenosides in Dingzhi Xiaowan[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 7-13.
汪鼎, 刘晓闯, 纪娟, 等. 使药石菖蒲对定志小丸中人参皂苷类成分肠吸收的影响[J]. 中国实验方剂学杂志, 2019,25(8):7-13. DOI： 10.13422/j.cnki.syfjx.20190552.

Ding WANG, Xiao-chuang LIU, Juan JI, et al. Effect of Acori Tatarinowii Rhizoma on Intestinal Absorption of Ginsenosides in Dingzhi Xiaowan[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 7-13. DOI： 10.13422/j.cnki.syfjx.20190552.

摘要

目的：

探究使药石菖蒲对定志小丸中人参皂苷成分肠吸收的影响，从肠吸收角度揭示石菖蒲“佐使则有功”的科学内涵。

方法：

采用超高效液相色谱-串联质谱法(UPLC-MS/MS)测定大鼠样品中人参皂苷Rg

的浓度；通过大鼠在体肠灌流试验比较不同配伍方式下定志小丸中3种人参皂苷类成分肠吸收速率常数(

)与表观通透系数(

app

)的差异；通过大鼠外翻肠囊试验考察不同剂量石菖蒲挥发油及P-糖蛋白(P-gp)抑制剂维拉帕米对3种人参皂苷类成分累积吸收量的影响。

结果：

缺失石菖蒲条件下定志小丸中3种人参皂苷类成分的

app

　均≤0.191×10

-3

cm·min

-1

。与缺石菖蒲组相比，缺石菖蒲挥发油组的

和

app

无显著性差异，但定志小丸全方组的

提高了3.97～8.35倍，

app

　提高了3.99～8.49倍；外翻肠囊研究表明定志小丸中石菖蒲挥发油能显著促进3种人参皂苷类成分在大鼠肠道的累积吸收量，但没有剂量依赖性。

结论：

石菖蒲挥发油可明显促进定志小丸中3种人参皂苷类成分的肠吸收，其促吸收机制可能与抑制P-gp有关。

Abstract

Objective:

To explore the effect of Acori Tatarinowii Rhizoma on intestinal absorption of ginsenosides in Dingzhi Xiaowan

and reveal the mechanism of Acori Tatarinowii Rhizoma acting as " adjuvant drug" in this formula.

Method:

The contents of ginsenoside Rg

Re and Rb

were measured by UPLC-MS/MS and the absorption of three ginsenosides in different intestine segments was investigated by rat single pass intestinal perfusion

in situ

including absorption rate constant(

) and apparent permeability coefficient(

app

). Everted intestinal sac model was used to investigate the absorption dosage of three ginsenosides affected by volatile oil from Acori Tatarinowii Rhizoma and verapamil[Ver

a P-glycoprotein(P-gp) inhibitor].

Result:

app

values of three ginsenosides were ≤0.191×10

-3

cm·min

-1

in Dingzhi Xiaowan when lack of Acori Tatarinowii Rhizoma.Compared with lack of Acori Tatarinowii Rhizoma in Dingzhi Xiaowan group

the

and

app

values of lack of volatile oil from Acori Tatarinowii Rhizoma in Dingzhi Xiaowan group slightly increased without significant difference in the four intestinal segments

but when the prescription had Acori Tatarinowii Rhizoma

the

increased by 3.97-8.35 fold and the

app

increased by 3.99-8.49 fold.The results of everted intestinal sac test showed that volatile oil of Acori Tatarinowii Rhizoma could significantly promote the intestinal absorption of ginsenoside Rg

Re and Rb

but there was no dose-dependent.

Conclusion:

Volatile oil of Acori Tatarinowii Rhizoma can promote the intestinal absorption of three ginsenosides in Dingzhi Xiaowan

and the mechanism may be related to the inhibiting function on P-gp.

关键词

Keywords

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