基于LC-MS的代谢组学技术分析孜亚比提片的降糖作用机制

张彩娟; 晋小雁; 邱敏懿; 张驰; 罗凌龙; 王敏; 赵保胜; 王学勇

doi:10.13422/j.cnki.syfjx.20190604

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基于LC-MS的代谢组学技术分析孜亚比提片的降糖作用机制

数据挖掘 | 更新时间：2021-02-09

- 基于LC-MS的代谢组学技术分析孜亚比提片的降糖作用机制
- Hypoglycemic Mechanism of Ziya Biti Tablet Based on LC-MS Metabolomics Technique
- 中国实验方剂学杂志 2019年25卷第8期页码：141-147
- 作者机构：
  
  1.北京中医药大学　中药学院，北京　 102488；
  2.北京中医药大学　北京中医药研究院，北京　 100029
- 作者简介：
  
  张彩娟，在读硕士，从事民族药药效及代谢组学研究，E-mail: 18513031449@126.com
  王学勇，博士，教授，博士生导师，从事药用植物与分子生药学，民族药新药研发工作，Tel: 010-64287660，E-mail: wxyph.d@163.com
- 基金信息：
  
  中央本级重大增减支项目(2060302);国家自然科学基金项目(81773841)
- DOI：10.13422/j.cnki.syfjx.20190604
  中图分类号： R2-0;R22;R285.5;R289
- 收稿日期：2018-09-02，
  
  网络出版日期：2018-12-05，
  
  纸质出版日期：2019-04-20
- 稿件说明：
移动端阅览
张彩娟, 晋小雁, 邱敏懿, 等. 基于LC-MS的代谢组学技术分析孜亚比提片的降糖作用机制[J]. 中国实验方剂学杂志, 2019,25(8):141-147.

Cai-juan ZHANG, Xiao-yan JIN, Min-yi QIU, et al. Hypoglycemic Mechanism of Ziya Biti Tablet Based on LC-MS Metabolomics Technique[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 141-147.
张彩娟, 晋小雁, 邱敏懿, 等. 基于LC-MS的代谢组学技术分析孜亚比提片的降糖作用机制[J]. 中国实验方剂学杂志, 2019,25(8):141-147. DOI： 10.13422/j.cnki.syfjx.20190604.

Cai-juan ZHANG, Xiao-yan JIN, Min-yi QIU, et al. Hypoglycemic Mechanism of Ziya Biti Tablet Based on LC-MS Metabolomics Technique[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 141-147. DOI： 10.13422/j.cnki.syfjx.20190604.

摘要

目的：

观察孜亚比提片对2型糖尿病大鼠的降糖效果，并基于代谢组学技术分析其降糖作用机制。

方法：

根据临床调研结果对不同组分来源的孜亚比提片进行筛选，筛选出最佳组方配比；并以2型糖尿病大鼠为实验对象进行药效学实验研究，正常组和模型组分别给予相同剂量的生理盐水、孜亚比提片组给予300 mg·kg

-1

孜亚比提片、二甲双胍组给予300 mg·kg

-1

盐酸二甲双胍片，每组8只，记录给药后实验组的空腹血糖值及体质量变化并进行数据分析；同时利用超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱联用(UHPLC-LTQ Orbitrap MS)技术对大鼠血清进行代谢组学分析，采用主成分分析(PCA)，偏最小二乘判别分析(OPLS-DA)对不同组别大鼠血清代谢物进行分析，鉴定出潜在生物标记物，并分析维吾尔族药孜亚比提片的降糖作用机制。

结果：

与正常组比较，模型组大鼠的空腹血糖值升高(

0.01)；与模型组比较，孜亚比提片组大鼠状态整体较好，毛色光滑，其空腹血糖值也有所下降(

0.05)，表明孜亚比提片对2型糖尿病大鼠有一定的降糖作用；根据LC-MS代谢组学数据结果最终得到8个生物标记物。与模型组相比孜亚比提片组中L-valine

propionylcarnitine含量均降低(

0.05)

sphingosine-1-phosphate

LPC(16∶1/0∶0)

LPC(18∶0/0∶0)

LPC(18∶2/0∶0)

LPC(20∶1/0∶0)

PC(19∶0/0∶0)的含量均升高(

0.05)。

结论：

孜亚比提片能够降低2型糖尿病大鼠血糖值，并能改善2型糖尿病大鼠的一般生理特征，其作用机制可能与改善氨基酸代谢，脂质代谢有关。

Abstract

Objective:

To observe the hypoglycemic effect of Uygur medicine Ziya Biti tablet on the type 2 diabetic rats

and analyze the hypoglycemic mechanism based on metabolomic techniques.

Method:

According to the results of clinical research about different origins of Ziya Biti tablet

the optimal composition was screened out; type 2 diabetic rats were taken as an experimental object in the pharmacodynamic experiments; the control group and model group were given the same dose of normal saline

Ziya Biti bablet group was given 300 mg·kg

-1

the metformin group was given 300 mg·kg

-1

metformin hydrochloride. The fasting blood and weight changes of the experimental group after the treatment were recorded and compared with normal group; ultra-high performance liquid chromatography-linear ion trap/electrostatic field orbit trap combined-type high resolution mass spectrometry (UHPLC-LTQ/Orbitrap MS) technology was used to conduct the metabolomics analysis on the rat serum

and principal component analysis (PCA)

partial least squares discriminant analysis (OPLS-DA) on different groups of rat serum metabolites were performed to identify potential biomarkers.

Result:

Compared with the model group

the rats in the Uygur medicine Ziya Biti tablet showed a healthy states

and the blood glucose were decreased(

0.05)

which indicated that Uygur medicine Ziya Biti tablet had a certain hypoglycemic effect on type 2 diabetic rats. Eight biomarkers were finally obtained according to the results. Compared with model group

L-valine and propionylcarnitine in the Uygur medicine group were decreased(

0.05)

while sphingosine-1-phosphate

LPC (16∶1/0∶0)

LPC (18∶0/0∶0)

LPC (18∶2/0∶0)

LPC 20∶1/0∶0)

PC (19∶0/0∶0) were elevated(

0.05).

Conclusion:

The experimental results showed that Uygur medicine Ziya Biti tablet can reduce the blood glucose of type 2 diabetic rats and allivate general physiological characteristics. The mechanism of action may be related to the improvement of amino acid metabolism and lipid metabolism.

关键词

Keywords

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