Objective:

2

To study the antibacterial activity and chemical constituents of Sojae Semen Praeparatum as a traditional Chinese medicine.

Method:

2

Sojae Semen Praeparatum was extracted with 80%ethanol

and the extracts were suspended in an appropriate amount of distilled water and extracted with petroleum ether

ethyl acetate and water saturated

n

-butanol in turn. The antibacterial activity

in vitro

was used for screening the active fractions. Using activity-guided-isolation

the active fractions were isolated and purified by silica gel column chromatography and preparative HPLC and other methods. Their structures were identified by physicochemical properties and spectroscopic analysis

such as

1

H-NMR

13

C-NMR and MS.

Result:

2

The ethyl acetate fraction of 80%ethanol extract from Sojae Semen Praeparatum showed a stronger activity and was studied further. Eighteen compounds were isolated

and eleven compounds were identified as daidzein (1)

daidzin (2)

genistein (3)

genistin (4)

glycitein (5)

glycitin (6)

daucosterol (7)

thymine (8)

adenine (9)

uracil (10)

uridine (11).

Conclusion:

2

The ethyl acetate fraction of 80%ethanol extract from Sojae Semen Praeparatum shows an antibacterial activity. Compounds 1-6 are isoflavones. Compounds 7-11 are isolated from Sojae Semen Praeparatum for the first time.