Objective:

2

To study on the correlation between integrated pharmacokinetics and pharmacodynamic effects of five active components(oxidized paeoniflorin

paeoniflorin

quercetin

gallic acid

paeonol) in Moutan Cortex.

Method:

2

Rats were divided into blank group

model group(syndrome of blood-heat and blood stasis) and drug-administered group.The concentration of five active components in serum were detected with UPLC-MS at different time points after being administrated ethanol extract of Moutan Cortex.The integrated concentrations were calculated according to area under the curve(AUC) self-defined weighting coefficiency.At the same time

the enzyme-linked immunosorbent assay(ELISA) was used to determine the contents of thromboxane B

2

(TXB

2

) and 6-keto-prostaglandin F

1

α

(6-keto-PGF

1

α

) in serum at different time points

and then correlation between pharmacodynamics and integrated pharmacokinetics of these five active ingredients was analyzed.

Result:

2

At different time points(0.083

0.25

0.5

0.75

1

2

3

4

6

8

10

12 h)

the integrated plasma concentrations of these five active ingredients in Moutan Cortex(158.65

174.60

220.13

227.23

244.31

251.51

404.28

654.39

472.62

355.04

231.56

199.40 mg·L

-1

) had a good correlation with concentration of TXB

2

(264.44

261.03

284.93

273.30

264.04

278.90

274.83

303.58

260.03

264.78

264.40

256.62 μg·L

-1

) and value of TXB

2

/6-keto-PGF

l

α

(4.50

4.47

3.66

3.37

3.29

3.66

3.71

4.30

3.63

3.65

3.75

3.66).

Conclusion:

2

There is a good correlation between the dynamic changes

in vivo

of active components from Moutan Cortex and pharmacodynamic effects of activating blood circulation of this herb.