辣椒碱通过下调SIRT1表达抑制乳腺癌MCF-7细胞的迁移和侵袭

毛岸云; 陈茂剑; 覃庆洪; 肖潺潺; 蒋玮; 梁至洁; 谭启杏; 杨伟萍; 韦长元

doi:10.13422/j.cnki.syfjx.20190722

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辣椒碱通过下调SIRT1表达抑制乳腺癌MCF-7细胞的迁移和侵袭

辣椒碱抗肿瘤研究专题 | 更新时间：2021-02-09

- 辣椒碱通过下调SIRT1表达抑制乳腺癌MCF-7细胞的迁移和侵袭
- Effect of Capsaicin in Inhibiting Migration and Invasion of Breast Cancer MCF-7 Cells by Down-regulating SIRT1
- 中国实验方剂学杂志 2019年25卷第7期页码：94-99
- 作者机构：
  
  广西医科大学　附属肿瘤医院，南宁　 530021
- 作者简介：
  
  毛岸云，在读硕士，副主任医师，从事乳腺癌的生物学特性与临床研究，E-mail: maoanyun@163.com
  韦长元，博士生导师，主任医师，从事乳腺癌的生物学特性与临床研究，E-mail:weicy63@aliyun.com
- 基金信息：
  
  广西科技基础条件平台建设项目(15-235-05);广西自然科学基金项目(2015GXNSFAA139204);广西研究生教育创新计划项目(YCSW2017108;YCBZ2018041);国家自然科学基金项目(81360396;8186034)
- DOI：10.13422/j.cnki.syfjx.20190722
  中图分类号： R22;R242;R2-031;R285.5
- 收稿日期：2018-10-11，
  
  网络出版日期：2018-12-20，
  
  纸质出版日期：2019-04-05
- 稿件说明：
移动端阅览
毛岸云, 陈茂剑, 覃庆洪, 等. 辣椒碱通过下调SIRT1表达抑制乳腺癌MCF-7细胞的迁移和侵袭[J]. 中国实验方剂学杂志, 2019,25(7):94-99.

An-yun MAO, Mao-jian CHEN, Qing-hong QIN, et al. Effect of Capsaicin in Inhibiting Migration and Invasion of Breast Cancer MCF-7 Cells by Down-regulating SIRT1[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 94-99.
毛岸云, 陈茂剑, 覃庆洪, 等. 辣椒碱通过下调SIRT1表达抑制乳腺癌MCF-7细胞的迁移和侵袭[J]. 中国实验方剂学杂志, 2019,25(7):94-99. DOI： 10.13422/j.cnki.syfjx.20190722.

An-yun MAO, Mao-jian CHEN, Qing-hong QIN, et al. Effect of Capsaicin in Inhibiting Migration and Invasion of Breast Cancer MCF-7 Cells by Down-regulating SIRT1[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 94-99. DOI： 10.13422/j.cnki.syfjx.20190722.

摘要

目的：

观察辣椒碱对人乳腺癌MCF-7细胞迁移和侵袭的影响，并初步探讨其分子生物学机制。

方法：

设立辣椒碱(25，50，75 μmol·L

－1

)组以及空白组，采用穿透小室(Transwell)迁移和侵袭实验分别检测辣椒碱(25，50，75 μmol·L

－1

)干预乳腺癌MCF-7细胞24 h后和空白组细胞的迁移和侵袭能力；实时荧光定量聚合酶链式反应(Real-time PCR)检测辣椒碱(25，50，75 μmol·L

－1

)干预乳腺癌MCF-7细胞24 h后和空白组细胞中沉默信息调节因子2同源蛋白1(SIRT1)和DNA聚合酶

催化亚基p125的编码基因POLD1(POLD1)mRNA表达水平；蛋白免疫印迹法(Western blot)检测辣椒碱(25，50，75 μmol·L

－1

)干预乳腺癌MCF-7细胞24 h后和空白组细胞中SIRT1和DNA聚合酶

催化亚基p125(p125)的蛋白表达水平。

结果：

与空白组比较，辣椒碱(25，50，75 μmol·L

－1

)干预乳腺癌MCF-7细胞24 h后，穿膜细胞数明显减少，迁移率和侵袭率显著降低，且呈浓度依赖效应(

0.01)；与空白组比较，辣椒碱(25，50，75 μmol·L

－1

)干预24 h能显著下调乳腺癌MCF-7细胞中SIRT1

POLD1 mRNA表达水平(

0.01)；与空白组比较，辣椒碱(25，50，75 μmol·L

－1

)干预24 h能显著降低乳腺癌MCF-7细胞中SIRT1和DNA聚合酶

催化亚基p125的蛋白表达水平(

0.01)。

结论：

辣椒碱能显著抑制乳腺癌MCF-7细胞的迁移和侵袭能力，其机制可能与下调细胞中SIRT1 mRNA和蛋白的表达水平以及POLD1 mRNA和p125的蛋白表达水平有关。

Abstract

Objective:

To investigate the effect of capsaicin on the migration and invasion of human breast cancer MCF-7 cells and the underlying molecular mechanism.

Method:

Three capsaicin intervention groups of different concentrations (25

75 μmol·L

-1

) and a blank group were set up. After MCF-7 cells were treated with different concentrations of capsaicin (25

75 μmol·L

-1

) for 24 h

the cell migration and invasion abilities were assessed by Transwell migration and invasion assay

respectively. Meanwhile

the mRNA level of silent information regulator 2 homolog 1 (SIRT1) and DNA polymerase

catalytic subunit p125 encoding gene POLD1 (POLD1) were detected by Real-time polymerase chain reaction (Real-time PCR). The protein levels of SIRT1 and DNA polymerase

catalytic subunit p125 (p125) were detected by Western blot.

Result:

Compared with the blank group

the number of transmembrane cells was significantly reduced

and the mobility was significantly decreased (

0.01) in a dose-dependent manner after the intervention of capsaicin (25

75 μmol·L

-1

) in MCF-7 cells for 24 h. Capsaicin (25

75 μmol·L

-1

) significantly down-regulated the mRNA and protein expressions of SIRT1 (

0.01) after intervention of capsaicin (25

75 μmol·L

-1

) in MCF-7 cells for 24 h. Furthermore

capsaicin (25

75 μmol·L

-1

) also significantly down-regulated the mRNA expression of POLD1 and the protein expression of p125 (

0.01) after intervention of capsaicin (25

75 μmol·L

-1

) in MCF-7 cells for 24 h.

Conclusion:

Capsaicin remarkably inhibits the cell migration and invasion of breast cancer MCF-7 cells

and the possible mechanism may be related to the down-regulation of SIRT1 and POLD1 mRNA expression levels and SIRT1 and p125 protein expression levels.

关键词

Keywords

references

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