辣椒碱对乳腺癌MDA-MB-231细胞裸鼠移植瘤生长的抑制作用

陈茂剑; 蒋玮; 毛岸云; 覃庆洪; 谭启杏; 肖潺潺; 梁至洁; 杨伟萍; 韦长元

doi:10.13422/j.cnki.syfjx.20190723

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辣椒碱对乳腺癌MDA-MB-231细胞裸鼠移植瘤生长的抑制作用

辣椒碱抗肿瘤研究专题 | 更新时间：2021-02-09

- 辣椒碱对乳腺癌MDA-MB-231细胞裸鼠移植瘤生长的抑制作用
- Inhibitory Effect of Capsaicin on Growth of Breast Cancer MDA-MB-231 Cells Transplanted Tumor in Nude Mice
- 中国实验方剂学杂志 2019年25卷第7期页码：82-88
- 作者机构：
  
  广西医科大学　附属肿瘤医院，南宁　 530021
- 作者简介：
  
  陈茂剑，在读博士，住院医师，从事乳腺癌的生物学特性与临床研究，E-mail: chenmj91@foxmail.com
  韦长元，博士，教授，主任医师，从事乳腺癌的生物学特性与临床研究，E-mail:weicy63@aliyun.com
- 基金信息：
  
  国家自然科学基金项目(81360396;81860341);广西科技基础条件平台建设项目(15-235-05);广西自然科学基金项目(2015GXNSFAA139204);广西研究生教育创新计划项目(YCSW2017108;YCBZ2018041)
- DOI：10.13422/j.cnki.syfjx.20190723
  中图分类号： R22;R242;R2-031;R285.5
- 收稿日期：2018-10-11，
  
  网络出版日期：2018-12-20，
  
  纸质出版日期：2019-04-05
- 稿件说明：
移动端阅览
陈茂剑, 蒋玮, 毛岸云, 等. 辣椒碱对乳腺癌MDA-MB-231细胞裸鼠移植瘤生长的抑制作用[J]. 中国实验方剂学杂志, 2019,25(7):82-88.

Mao-jian CHEN, Wei JIANG, An-yun MAO, et al. Inhibitory Effect of Capsaicin on Growth of Breast Cancer MDA-MB-231 Cells Transplanted Tumor in Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 82-88.
陈茂剑, 蒋玮, 毛岸云, 等. 辣椒碱对乳腺癌MDA-MB-231细胞裸鼠移植瘤生长的抑制作用[J]. 中国实验方剂学杂志, 2019,25(7):82-88. DOI： 10.13422/j.cnki.syfjx.20190723.

Mao-jian CHEN, Wei JIANG, An-yun MAO, et al. Inhibitory Effect of Capsaicin on Growth of Breast Cancer MDA-MB-231 Cells Transplanted Tumor in Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 82-88. DOI： 10.13422/j.cnki.syfjx.20190723.

摘要

目的：

观察辣椒碱对乳腺癌MDA-MB-231细胞裸鼠移植瘤生长的抑制作用及其机制。

方法：

通过皮下注射MDA-MB-231细胞的方法建立乳腺癌裸鼠移植瘤模型，待肿瘤体积达到约100 mm

，将裸鼠随机分为模型组和辣椒碱低、中、高剂量(5

20 mg·kg

－1

)组，每组5只。辣椒碱低、中、高剂量(5

20 mg·kg

－1

)组裸鼠分别腹腔注射相应剂量的辣椒碱，模型组注射等体积的磷酸缓冲盐溶液(PBS)溶剂，3 d/次，连续给药8次。每次给药前先检测裸鼠体质重和肿瘤体积，末次给药24 h后处死各组裸鼠，计算肿瘤体积，称肿瘤质量，并计算抑瘤率。应用免疫组化法和蛋白免疫印迹法(Western blot)检测辣椒碱治疗后各组肿瘤组织中高迁移率族蛋白B1(HMGB1)和Toll样受体4(TLR4)蛋白表达水平。

结果：

与模型组比较，辣椒碱低、中、高剂量(5，10，20 mg·kg

－1

)组裸鼠体质量无明显变化，但肿瘤体积显著缩小(

0.01)，肿瘤质量显著减小(

0.01)，抑瘤率显著增大(

0.01)，且呈剂量依赖性；与模型组比较，辣椒碱低、中、高剂量(5

20 mg·kg

－1

)组肿瘤组织HMGB1和TLR4蛋白表达明显降低，且呈剂量依赖性。

结论：

辣椒碱对乳腺癌MDA-MB-231细胞裸鼠移植瘤生长具有明显的抑制作用，其机制可能与下调HMGB1和TLR4蛋白表达有关。

Abstract

Objective:

To investigate the inhibitory effect of capsaicin on the growth of breast cancer MDA-MB-231 cells transplanted tumour in nude mice and its possible molecular mechanism.

Method:

Transplanted tumor model of breast cancer MDA-MB-231 cells in nude mice were established. Then the tumor-bearing mice were randomly divided into 4 groups: model group

and low

medium and high-dose capsaicin groups (5

20 mg·kg

-1

). Mice of low

medium and high-dose capsaicin groups (5

20 mg·kg

-1

) were intraperitoneally injected with the corresponding dose of capsaicin

and the model group was injected with the same volume of phosphate buffer saline (PBS)

once every 3 days

for a total of 8 times in succession. Body weight of mice and transplantation tumor volume were measured before each injection of capsaicin. Mice of each group were put to death 24 h after the last administration

and then the tumor volume

mass and the tumor inhibitory rate were calculated. The protein expression levels of high mobility group box 1 (HMGB1) and Toll-like receptors 4(TLR4) were measured by immunohistochemistry and Western blot.

Result:

No significant difference was observed between each group in body weight. However

compared with the model group

capsaicin (5

20 mg·kg

-1

) remarkably inhibited the tumor volume and mass (

0.05

0.01)

and increased the tumor inhibitory rate (

0.01) in a dose-dependent manner. Capsaicin (5

20 mg·kg

-1

) also markedly inhibited the protein expression levels of HMGB1 and TLR4 (

0.01).

Conclusion:

Capsaicin remarkably inhibits the growth of breast cancer MDA-MB-231 cells transplanted tumour in nude mice

and the possible mechanism may be related to the down-regulation of HMGB1 and TLR4 at the protein level.

关键词

Keywords

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