Wnt/PCP信号通路中RhoA mRNA与AECOPD中医证型的相关性

张军; 葛正行; 周洵; 杨义

doi:10.13422/j.cnki.syfjx.20190726

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Wnt/PCP信号通路中RhoA mRNA与AECOPD中医证型的相关性

临床 | 更新时间：2021-02-09

- Wnt/PCP信号通路中RhoA mRNA与AECOPD中医证型的相关性
- Correlation Between RhoA Gene in Wnt/PCP Signaling Pathway and Traditional Chinese Medicine Syndrome of AECOPD
- 中国实验方剂学杂志 2019年25卷第16期页码：73-77
- 作者机构：
  
  贵阳中医学院　第二附属医院，贵阳　 550003
- 作者简介：
  
  张军，硕士，住院医师，从事呼吸系统疾病的研究与防治，E-mail：1065882086@qq.com
  葛正行，主任医师，教授，从事呼吸系统疾病的临床与基础研究，E-mail：2562900350@qq.com
- 基金信息：
  
  国家自然科学基金项目(81473533;81260599);贵州省优秀科技教育人才省长专项资金项目(黔省专合字【2011】62号)
- DOI：10.13422/j.cnki.syfjx.20190726
  中图分类号： R22; R242; R2-031; R287;R735.7
- 收稿日期：2018-07-21，
  
  网络出版日期：2018-12-20，
  
  纸质出版日期：2019-08-20
- 稿件说明：
移动端阅览
张军, 葛正行, 周洵, 等. Wnt/PCP信号通路中RhoA mRNA与AECOPD中医证型的相关性[J]. 中国实验方剂学杂志, 2019,25(16):73-77.

Jun ZHANG, Zheng-xing GE, Xun ZHOU, et al. Correlation Between RhoA Gene in Wnt/PCP Signaling Pathway and Traditional Chinese Medicine Syndrome of AECOPD[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 73-77.
张军, 葛正行, 周洵, 等. Wnt/PCP信号通路中RhoA mRNA与AECOPD中医证型的相关性[J]. 中国实验方剂学杂志, 2019,25(16):73-77. DOI： 10.13422/j.cnki.syfjx.20190726.

Jun ZHANG, Zheng-xing GE, Xun ZHOU, et al. Correlation Between RhoA Gene in Wnt/PCP Signaling Pathway and Traditional Chinese Medicine Syndrome of AECOPD[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 73-77. DOI： 10.13422/j.cnki.syfjx.20190726.

摘要

目的：

探讨平面细胞极性(Wnt/PCP)信号通路中Ras homolog gene family member A(RhoA) mRNA与慢性阻塞性肺疾病急性加重期(AECOPD)中医证型的相关性，尝试为AECOPD中医证型诊断提供客观标准。

方法：

收集AECOPD患者100例，分为痰浊壅肺证、痰热郁肺证、痰蒙神窍证、肺肾气虚证、阳虚水泛证5个组别，每组20例。正常组15例。所有入选患者空腹抽取静脉血，血液总RNA的提取采用试剂盒，按说明书进行操作，采用实时荧光定量聚合酶链式反应(Real-time PCR)检测RhoA基因在AECOPD中医证型患者血液中mRNA的表达量，并探讨其相关性。

结果：

痰热郁肺组和痰蒙神窍组比较无差异；与正常组比较，痰浊壅肺组、痰热郁肺组、痰蒙神窍组、肺肾气虚组和阳虚水泛组中RhoA mRNA表达量明显增高(

0.01)；5组证型间RhoA mRNA表达量，痰浊壅肺组

痰热郁肺组/痰蒙神窍组

肺肾气虚组

阳虚水泛组。RhoA mRNA相对表达量与AECOPD 5组中医证型之间存在直线线性相关关系，且呈正性相关。

结论：

Wnt/PCP信号通路中RhoA mRNA的相对表达量在5组中医证型中存在显著差异，可能给AECOPD中医证型诊断提供一定的客观标准，并揭示其疾病严重程度。

Abstract

Objective:

To investigate the correlation between Ras homolog gene family member A (RhoA) gene in Wnt/PCP signaling pathway and acute exacerbation chronic obstructive pulmonary disease (AECOPD) traditional Chinese medicine(TCM)syndrome

attempting to provide an objective standard for the diagnosis of AECOPD TCM syndrome.

Method:

The 100 AECOPD patients were collected and divided into 5 groups: phlegm turbid obstructing lung syndrome

phlegm-heat obstructing lung syndrome

syndrome of orifices confused by phlegm

deficiency of pulmonary and renal Qi

and edema due to yang deficiency

with 20 people in each group. 15 normal people were selected as a normal control group. All patients received fasting hemospasia

using a kit to extract blood total RibonucleicA(RNA) according to instructions. Real-time quantitative polymerase chain reaction (Real-time PCR) was adopted to detect the mRNA expression of RhoA gene in blood of patients with AECOPD TCM syndrome

and to explore the correlation.

Result:

There was no difference between phlegm-heat obstructing lung syndrome group and syndrome of orifices confused by phlegm group. The mRNA expression of RhoA gene in phlegm turbid obstructing lung syndrome group

phlegm-heat obstructing lung syndrome group

syndrome of orifices confused by phlegm group

deficiency of pulmonary and renal Qi group

and edema due to Yang deficiency group were significantly higher than that in normal group (

0.01). The mRNA expression of RhoA gene in five TCM syndrome groups was as follows: phlegm turbid obstructing lung syndrome

phlegm-heat obstructing lung syndrome / syndrome of orifices confused by phlegm

deficiency of pulmonary renal Qi

edema due to Yang deficiency. There was a positive linear correlation between the relative expression of RhoA mRNA and above five AECOPD TCM syndrome groups.

Conclusion:

The significant difference in mRNA relative expression of RhoA gene in Wnt / PCP signaling pathway among the five AECOPD TCM syndrome groups may provide some objective diagnostic criteria for AECOPD TCM syndromes and reveal their disease severity.

关键词

Keywords

references

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