桃仁承气汤对脓毒症大鼠肠黏膜屏障及TLR9信号通路的影响

孙治霞; 索红亮; 王丽辉

doi:10.13422/j.cnki.syfjx.20190738

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桃仁承气汤对脓毒症大鼠肠黏膜屏障及TLR9信号通路的影响

经典名方 | 更新时间：2021-02-09

- 桃仁承气汤对脓毒症大鼠肠黏膜屏障及TLR9信号通路的影响
- Effects of Taoren Chengqitang on Intestinal Mucosal Barrier and TLR9 Signaling Pathway in Septic Rats
- 中国实验方剂学杂志 2019年25卷第7期页码：29-35
- 作者机构：
  
  河南省中医院，郑州　 450002
- 作者简介：
  
  孙治霞，硕士，主治医师，从事心血管病临床研究，E-mail:jameszll@yeah.net
- 基金信息：
  
  河南省中医药科学研究专项(2018ZY2050)
- DOI：10.13422/j.cnki.syfjx.20190738
  中图分类号： R289;R574.4;R364.5
- 收稿日期：2018-10-17，
  
  网络出版日期：2018-12-18，
  
  纸质出版日期：2019-04-05
- 稿件说明：
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孙治霞, 索红亮, 王丽辉. 桃仁承气汤对脓毒症大鼠肠黏膜屏障及TLR9信号通路的影响[J]. 中国实验方剂学杂志, 2019,25(7):29-35.

Zhi-xia SUN, Hong-liang SUO, Li-hui WANG. Effects of Taoren Chengqitang on Intestinal Mucosal Barrier and TLR9 Signaling Pathway in Septic Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 29-35.
孙治霞, 索红亮, 王丽辉. 桃仁承气汤对脓毒症大鼠肠黏膜屏障及TLR9信号通路的影响[J]. 中国实验方剂学杂志, 2019,25(7):29-35. DOI： 10.13422/j.cnki.syfjx.20190738.

Zhi-xia SUN, Hong-liang SUO, Li-hui WANG. Effects of Taoren Chengqitang on Intestinal Mucosal Barrier and TLR9 Signaling Pathway in Septic Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 29-35. DOI： 10.13422/j.cnki.syfjx.20190738.

摘要

目的：

探讨桃仁承气汤对脓毒症大鼠肠黏膜屏障的保护作用以及作用机制。

方法：

将大鼠分为假手术组，模型组（盲肠结扎穿孔法复制脓毒症大鼠），桃仁承气汤低、中、高剂量组（2.85，5.70，8.55 g·kg

－1

），地塞米松组（0.01 g·kg

－1

），每组12只。末次给药结束后，处死大鼠，电镜下观察肠黏膜形态变化；检测肠系膜淋巴结、肝、肾、脾组织细菌移位率；酶联免疫吸附测定（ELISA）检测血清肿瘤坏死因子-

（TNF-

），白细胞介素-1

（IL-1

）以及小肠组织中二胺氧化酶（DAO），肠型脂肪酸结合蛋白（i-FABP）水平；蛋白免疫印迹法（Western blot），免疫组化法（IHC）检测小肠组织中Toll样受体9（TLR9），髓样分化因子88（MyD88），核转录因子-

B亚基p65（NF-

B p65）蛋白表达。

结果：

与假手术组比较，模型组大鼠器官细菌移位率，TNF-

，IL-1

水平明显升高，DAO，i-FABP水平，黏膜厚度，绒毛高度明显降低，TLR9，MyD88，细胞核NF-

B p65蛋白表达明显升高（

0.05，

0.01），细胞质NF-

B p65蛋白表达明显降低（

0.05）；与模型组比较，桃仁承气汤低、中、高剂量组，地塞米松组器官细菌移位率，TNF-

，IL-1

水平明显降低，DAO，i-FABP水平，黏膜厚度，绒毛高度明显升高，TLR9，MyD88，细胞核NF-

B p65蛋白表达明显降低（

0.05），细胞质NF-

B p65蛋白表达明显升高（

0.05）。

结论：

桃仁承气汤对脓毒症大鼠肠黏膜屏障具有一定的保护作用，这可能与抑制TLR9信号通路有关。

Abstract

Objective:

To study the protective effect of Taoren Chengqitang on intestinal mucosal barrier in septic rats and its possible mechanism.

Method:

Rats were divided into sham operation group

model group (replication of septic rats with cecal ligation and perforation)

low

middle and high-dose Taoren Chengqitang groups (2.85

5.70

8.55 g·kg

-1

)

and dexamethasone group (0.01 g·kg

-1

)

with 12 rats in each group. After last administration

rats were put to death

the morphological changes of intestinal mucosa were observed under electron microscope

the bacterial translocation rates in lymphoglandulae mesentericae

liver

kidney and spleen tissues were detected; the levels of serum tumor necrosis factor-

(TNF-

)

interleukin-1

(IL-1

)

and diamine oxidase (DAO) and intestinal fatty acid binding protein (i-FABP) levels in small intestine tissues were detected by enzyme-linked immunosorbent assay (ELISA). Expressions of Toll like receptor 9 (TLR9)

myeloid differentiation factor 88 (MyD88) and nuclear factor-kappa B subunit p65 in small intestine tissues were detected by Western blot and immunohistochemistry.

Result:

Compared with sham operated group

the bacterial translocation rate

TNF-

and IL-1

levels in model group increased significantly

DAO

i-FABP

mucosal thickness and villus height decreased significantly

and protein expressions of TLR9

MyD88 and nucleus NF-

B p65 increased significantly (

0.05

0.01)

and expression of cytoplasmic NF-

B p65 protein decreased significantly. Compared with model group

the bacterial translocation rate

TNF-

and IL-1

levels in organs of low

medium and high-dose Taoren Chengqitang groups and dexamethasone group decreased significantly

DAO

i-FABP

mucosal thickness and villus height increased significantly

while the protein expressions of TLR9

MyD88 and nucleus NF-

B p65 decreased significantly (

0.05)

and expression of cytoplasmic NF-

B p65 protein increased significantly.

Conclusion:

Taoren Chengqitang has a certain protective effect on intestinal mucosal barrier in septic rats

which may be related to the inhibition of TLR9 signaling pathway.

关键词

Keywords

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