黄曲霉毒素B<sub>1</sub>免疫快速检测技术研究进展及其在中药中的应用

单利楠; 豆小文; 刘好; 段亚萍; 杨世海; 杨美华

doi:10.13422/j.cnki.syfjx.20190811

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黄曲霉毒素B₁免疫快速检测技术研究进展及其在中药中的应用

综述 | 更新时间：2021-02-09

- 黄曲霉毒素B₁免疫快速检测技术研究进展及其在中药中的应用
- Rapid Immunoassay of Aflatoxin B₁ and Its Application in Traditional Chinese Medicine
- 中国实验方剂学杂志 2019年25卷第8期页码：194-209
- 作者机构：
  
  1.吉林农业大学　中药材学院，长春　 130118；
  2.中国医学科学院　北京协和医学院　药用植物研究所　中草药物质基础与资源利用教育部重点实验室，北京　 100193
- 作者简介：
  
  单利楠，在读硕士，从事中药中真菌毒素的检测研究，E-mail: shanlinan1021@163.com
  杨世海，教授，博士生导师，从事药用植物栽培与育种，Tel: 0431-84533358，E-mail: jlyangs@163.com；
  杨美华，研究员，博士生导师，从事中药质量分析与安全性研究，Tel: 010-57833277，E-mail: yangmeihua15@hotmail.com
- 基金信息：
  
  国家中药标准化项目(ZYBZH-Y-JIN-34);国家重点研发计划项目(2016YFE0112900);中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-012;2017-I2M-1-013)
- DOI：10.13422/j.cnki.syfjx.20190811
  中图分类号： R284.1;R289;R22;R2-031
- 收稿日期：2018-06-25，
  
  网络出版日期：2019-01-05，
  
  纸质出版日期：2019-04-20
- 稿件说明：
移动端阅览
单利楠, 豆小文, 刘好, 等. 黄曲霉毒素B₁免疫快速检测技术研究进展及其在中药中的应用[J]. 中国实验方剂学杂志, 2019,25(8):194-209.

Li-nan SHAN, Xiao-wen DOU, Hao LIU, et al. Rapid Immunoassay of Aflatoxin B₁ and Its Application in Traditional Chinese Medicine[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 194-209.
单利楠, 豆小文, 刘好, 等. 黄曲霉毒素B₁免疫快速检测技术研究进展及其在中药中的应用[J]. 中国实验方剂学杂志, 2019,25(8):194-209. DOI： 10.13422/j.cnki.syfjx.20190811.

Li-nan SHAN, Xiao-wen DOU, Hao LIU, et al. Rapid Immunoassay of Aflatoxin B₁ and Its Application in Traditional Chinese Medicine[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 194-209. DOI： 10.13422/j.cnki.syfjx.20190811.

摘要

黄曲霉毒素B

(aflatoxin B

，AFB

)是由产毒真菌(如黄曲霉和寄生霉菌)产生的有毒次级代谢产物，具有致癌、致畸、致突变作用。研究表明AFB

广泛存在于农作物、食品、饲料甚至中药中，对人类健康造成了严重的安全隐患。建立一系列适用于不同基质中AFB

的多种快速检测技术，为预防和控制食品与药品的安全、保障人类健康方面具有重要意义。随着小分子免疫技术的不断提高，近年来各种检测形式的AFB

免疫快速技术被开发利用到现场快速检测领域，该文系统综述了我国现行有效的AFB

检测相关标准及近年来一些地区和国家等对中药材中黄曲霉毒素的限量标准情况，这些限量标准主要针对食品、饲料及一些易污染中药材中的黄曲霉毒素，而研究表明除了限量标准进行规定的中药材品种外也存在一些药用植物及其产品也有被黄曲霉毒素污染的情况。并对AFB

抗原抗体制备技术，以及基于抗原抗体特异性识别功能的快速检测方法包括酶联免疫吸附法、亲和色谱法、荧光免疫法、化学发光免疫法和新型免疫传感器法的开发及应用进行了归纳总结与对比。以期为中药规范化种植、中药集散贸易市场、药店医院、中药质量监管等方面形成快速、准确、灵敏的检测技术标准提供参考，确保中药的质量安全。

Abstract

Aflatoxin B

(AFB

) is a toxic secondary metabolite produced by toxigenic

Aspergillus

spp. (such as

flavus

and

parasiticus

) with carcinogenic

teratogenic and mutagenic effects. Studies have shown that AFB

is widely found in crops

food

feed and traditional Chinese medicine

which poses a serious safety hazards to humans healthy. The establishment of a rapid detection technique that is suitable for AFB

in different matrices has a great significance in preventing contamination

controlling food and medicines safety and ensuring human health. With the continuous improvement of small-molecule immune technology

various rapid immunoassays of AFB

have been developed and utilized in recent years. This review systematically summarized current relevant standards for the detection of AFB

in China and the maximum recommended levels for the application of aflatoxins in Chinese herbal medicines in some regions and countries. These standards are mainly applicable for aflatoxins in food

feed and some easily contaminated samples of Chinese herbal medicines. Some studies have shown that except the Chinese herbal medicines specified with the maximum recommended levels

some medicinal herbs and their products were also contaminated by aflatoxins. In addition

this paper reviewed the preparation technology of antigen and antibody for AFB

and the rapid detection methods based on the specific recognition ability between antigen and antibody

including enzyme-linked immunosorbent assay

immunochromatographic assay

fluorescence immunoassay

chemiluminescence immunoassay

and novel immunosensor method

were also summarized and compared. This review aims to provide the reference for rapid

accurate

and sensitive technical standards for the detection of AFB

in traditional Chinese medicine during the agricultural planting

distribution

trade and quality supervision and for the market

pharmacies and hospitals

so as to ensure the quality and safety of the traditional Chinese medicines.

关键词

Keywords

references

胡一晨，万丽，范成杰，等．免疫亲和柱净化HPLC柱后光化学衍生化法检测中药及染菌中药制剂中间体的黄曲霉毒素 [J]. 中国实验方剂学杂志， 2012 , 18 ( 10 ): 116 - 119 .

马双成，金红宇，刘丽娜，等．中药中外源性有害物质残留风险控制初探 [J]. 中国药学杂志， 2015 , 50 ( 2 ): 99 - 103 .

韦日伟，杨小丽，仇峰，等．免疫亲和柱净化-在线柱后光化学衍生-HPLC-FLD同时测定甘草中黄曲霉毒素B 1 ，B 2 ，G 1 ，G 2 和赭曲霉毒素A的含量 [J]. 中国中药杂志， 2011 , 36 ( 17 ): 2342 - 2346 .

LIU H M , KONG W J , LIU C M , et al . Rapid analysis and identification of multi-class mycotoxins in Morinda officinalis by UFLC-ESI-MS/MS [J]. RSC Adv , 2015 , 5 ( 78 ): 63561 - 63571 .

YANG X , WEI R , SHEN H , et al . Determination of aflatoxins in animal medicines by immunoaffinity column cleanup and HPLC-FLD with post-column photochemical derivatization [J]. Chin Pharm , 2011 , 20 ( 15 ): 4 - 5 .

LIU S , QIU F , KONG W , et al . Development and validation of an accurate and rapid LC-ESI-MS/MS method for the simultaneous quantification of aflatoxin B 1 ，B 2 ，G 1 and G 2 in lotus seeds [J]. Food Control , 2013 , 29 ( 1 ): 156 - 161 .

LIU Y , WU F. Global burden of aflatoxin-lnduced hepatocellular carcinoma: A risk assessment [J]. Environ Health Persp , 2010 , 118 ( 6 ): 818 - 824 .

陈思颖，朱迪，王永林，等．免疫亲和柱净化UPLC-MS-MS测定天麻药材中黄曲霉毒素的含量 [J]. 中国实验方剂学杂志， 2014 , 20 ( 1 ): 51 - 55 .

罗朝权，张敏玲，郑润生，等 . 6种动物类药材中黄曲霉毒素污染的液质联用检测 [J]. 中国实验方剂学杂志， 2018 , 24 ( 3 ): 67 - 71 .

Smith R . Commission Regulation ( EU ) No 330/2010 [M]. Brussels : Core EU Legislation , 2015 .

张洪芳．解析《食品中真菌毒素限量》新标准 [J]. 食品安全导刊， 2013 ( Z1 ): 46 - 47 .

Ireland FSAO . Guidance note 26: Guidance for food business operators on the implementation of commission regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs [J]. 2011 .

李芬．卫生部公布4项食品安全国家标准 [J]. 中国食品学报， 2011 , 11 ( 3 ): 97 .

李峻媛，万丽，杨美华．真菌毒素限量标准及其在中药中的研究进展 [J]. 中草药， 2011 , 42 ( 3 ): 602 - 609 .

徐俊．韩国限制韩药材霉菌毒素限量的品种增加到19个 [J]. 中医药国际参考， 2008 ( 11 ): 8 .

章颖慧，王秀英，杨明，等．中药有害残留物限量制定原则及其影响因素 [J]. 药物分析杂志， 2015 , 35 ( 3 ): 558 - 567 .

魏继涛，胡延春，宁红梅，等．黄曲霉毒素B 1 完全抗原合成及鼠源多抗血清的制备 [J]. 中国农学通报， 2012 , 28 ( 14 ): 44 - 49 .

蔡敏，周常义，李健，等．黄曲霉毒素B 1 人工抗原的制备及鉴定 [J]. 湖南农业科学， 2013 ( 23 ): 10 - 13 .

伍鑫茹，杨雪娇，赵肃清，等．黄曲霉毒素B 1 人工抗原的合成及鉴定 [J]. 现代食品科技， 2013 , 29 ( 4 ): 796 - 799 .

陈宇熹，方振霞，雷美玲，等．黄曲霉毒素B 1 人工抗原的制备、鉴定与免疫特性研究 [J]. 武夷科学， 2017 , 33 ( 1 ): 80 - 87 .

Thirumala-Devi K , Miller J S , Reddy G , et al . Phage-displayed peptides that mimic aflatoxin B 1 in serological reactivity [J]. J Appl Microbiol , 2001 , 90 ( 3 ): 330 - 336 .

LIU R R , XU L , QIU X M , et al . An immunoassay for determining aflatoxin B 1 using a recombinant phage as a nontoxic coating conjugate [J]. J Food Safety , 2012 , 32 ( 3 ): 318 - 325 .

WANG Y , WANG H , LI P , et al . Phage-displayed peptide that mimics aflatoxins and its application in immunoassay [J]. J Agric Food Chem , 2013 , 61 ( 10 ): 2426 - 2433 .

王妍入．黄曲霉毒素替代物绿色免疫分析研究 [D]．北京：中国农业科学院， 2014 .

张甄．同时检测黄曲霉毒素B 1 和玉米赤霉烯酮免疫层析试纸条的研制 [D]．大庆：黑龙江八一农垦大学， 2010 .

王磊，胡骁飞，职爱民，等．黄曲霉毒素B 1 人工抗原及鼠源多克隆抗血清的制备 [J]. 食品科技， 2011 , 36 ( 3 ): 277 - 281 .

李敏．黄曲霉毒素及其前体杂色曲霉毒素高灵敏高特异性单克隆抗体研制 [D]．北京：中国农业科学院， 2014 .

ZHANG M Y , LU J J , WANG L , et al . Development of monoclonal antibodies in China: overview and prospects [J]. Biomed Res Int , 2015 , doi: 10.1155/2015/168935 doi:10.1155/2015/168935 .

孙宗棠，吴玉英．抗黄曲霉B 1 的单克隆抗体及其免疫测定体系 [J]. 医学研究通讯， 1983 ( 1 ): 11 .

李涛．抗黄曲霉毒素B单克隆抗体的制备与特性鉴定 [D]．济南：山东师范大学， 2008 .

ZHANG X , FENG M , LIU L , et al . Detection of aflatoxins in tea samples based on a class-specific monoclonal antibody [J]. Int J Food Sci Technol , 2013 , 48 ( 6 ): 1269 - 1274 .

ZHANG D , LI P , ZHANG Q , et al . Production of ultrasensitive generic monoclonal antibodies against major aflatoxins using a modified two-step screening procedure [J]. Anal Chim Acta , 2009 , 636 ( 1 ): 63 - 69 .

LI X , LI P , ZHANG Q , et al . Molecular characterization of monoclonal antibodies against aflatoxins: a possible explanation for the highest sensitivity [J]. Anal Chem , 2012 , 84 ( 12 ): 5229 - 5235 .

张勋，匡华，徐丽广，等．绿色无毒黄曲霉毒素B 1 免疫检测方法 [J]. 食品与生物技术学报， 2013 , 32 ( 12 ): 1293 - 1297 .

曹冬梅，许杨，涂追，等．基于纳米抗体-碱性磷酸酶融合蛋白的一步酶联免疫吸附分析法检测黄曲霉毒素B 1 [J]. 分析化学， 2016 , 44 ( 7 ): 1085 - 1091 .

李鑫，李培武，张奇，等．抗黄曲霉毒素单链抗体基因的克隆与表达 [J]. 中国油料作物学报， 2012 , 34 ( 5 ): 528 - 532 .

谢珲，章先，王歆，等．黄曲霉毒素B 1 单克隆抗体的制备及间接竞争ELISA检测技术研究 [J]. 微生物学通报， 2015 , 42 ( 10 ): 2033 - 2040 .

卢守英．黄曲霉毒素B 1 胶体金免疫层析试纸条的研制 [D]．扬州：扬州大学， 2010 .

李敏．黄曲霉毒素和杂色曲霉毒素单克隆抗体研制 [D]．北京：中国农业科学院， 2014 .

ZHANG D , LI P , YANG Y , et al . A high selective immunochromatographic assay for rapid detection of aflatoxin B 1 [J]. Talanta , 2011 , 85 ( 1 ): 736 - 742 .

Min W K , Kweon D H , Park K , et al . Characterisation of monoclonal antibody against aflatoxin B 1 ，produced in hybridoma 2C12 and its single-chain variable fragment expressed in recombinant Escherichia coli [J]. Food Chem , 2011 , 126 ( 3 ): 1316 - 1323 .

WANG L , HU X F , TENG M. Development of monoclonal antibody against aflatoxin B 1 and establishment of immunology quantitative ELISA [J]. Chin J Public Health , 2012 , 28 ( 1 ): 58 - 60 .

LI P , ZHANG Q , ZHANG W. Immunoassays for aflatoxins [J]. Trac Trend Anal Chem , 2009 , 28 ( 9 ): 1115 - 1126 .

王亚楠，王晓斐，牛琳琳，等．食品中黄曲霉毒素总量免疫分析方法研究进展 [J]. 食品工业科技， 2017 , 38 ( 13 ): 344 - 351 .

王静，王淼．我国食品安全快速检测技术发展现状研究 [J]. 农产品质量与安全， 2014 ( 2 ): 42 - 47 .

Shim W B , Kim K , Ofori J A , et al . Occurrence of aflatoxins in herbal medicine distributed in South Korea [J]. J Food Protect , 2012 , 75 ( 11 ): 1991 - 1999 .

Tosun H , Arslan R. Determination of aflatoxin B 1 levels in organic spices and herbs [J]. The Scientific World J , 2013 , doi: 10.1155/2013/874093 doi:10.1155/2013/874093 .

张振凌，陈红，石延帮．不同牛膝饮片黄曲霉毒素B 1 含量比较和限度的确定 [J]. 中成药， 2005 , 27 ( 5 ): 612 - 614 .

李延生，陈建民． ELISA 试剂盒定量检测中药材和中成药的黄曲霉毒素B 1 [J]. 中草药， 2000 , 31 ( 8 ): 586 - 587 .

梁月秋，黄荣芳．中药污染黄曲霉毒素B 1 检测分析 [J]. 中国现代应用药学， 2000 , 17 ( 3 ): 224 - 226 .

HU L L , XIANG Y Z , CAI Z X , et al . A comparative study on 4 kinds of aflatoxin B 1 assay kits and liquid chromatography [J]. J Food Saf Qua , 2014 , 5 ( 3 ): 813 - 818 .

SUN D , GU X , LI J , et al . Quality evaluation of five commercial enzyme linked immunosorbent assay kits for detecting aflatoxin B 1 in feedstuffs [J]. Asian-australas J Anim Sci , 2015 , 28 ( 5 ): 691 - 696 .

张爱婷，石延榜，张振凌，等． ELISA法测定部分种子和果实类中药黄曲霉毒素B 1 含量 [J]. 医学研究杂志， 2008 , 37 ( 10 ): 48 - 49 .

XIONG Y , PEI K , WU Y , et al . Colorimetric ELISA based on glucose oxidase-regulated the color of acid-base indicator for sensitive detection of aflatoxin B 1 ，in corn samples [J]. Food Control , 2017 , 78 : 317 - 323 .

XIE T B , LIU H , WEI L U , et al . Determination of aflatoxin B 1 in cooking oil, peanut, and grain by using direct competitive ELISA kit [J]. J Food Saf Qual , 2015 , 6 ( 7 ): 2834 - 2839 .

LIU B H , Hsu Y T , LU C C , et al . Detecting aflatoxin B 1 in foods and feeds by using sensitive rapid enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip [J]. Food Control , 2013 , 30 ( 1 ): 184 - 189 .

YU F Y , Gribas A V , Vdovenko M M , et al . Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin B 1 in food products [J]. Talanta , 2013 , 107 ( 5 ): 25 - 29 .

ZHANG H , MO H Z , LI H. Preparation of aflatoxin B 1 polyclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay [J]. J Food Agric Environ , 2013 , 11 ( 1 ): 190 - 194 .

褚先锋，豆小文，孔维军，等．间接竞争酶联免疫吸附法快速筛检莲子中黄曲霉毒素B 1 的污染水平 [J]. 中国中药杂志， 2015 , 40 ( 4 ): 704 - 709 .

邓娟．黄曲霉毒素B 1 酶联免疫检测方法研究 [D]．天津：天津科技大学， 2016 .

WAN Y P , LI Y , WANG F H , et al . Study and application on the ELISA Kit for detecting aflatoxins B 1 [J]. Chin Anim Husban Veter Med , 2013 , 40 ( 1 ): 57 - 61 .

柳桢．黄曲霉毒素B 1 免疫检测标准品替代研究 [D]．郑州：郑州大学， 2006 .

王文凤．真菌毒素寡核苷酸适配体的筛选与应用 [D]．无锡：江南大学， 2012 .

REN M , XU H , HUANG X , et al . Immunochromatographic assay for ultrasensitive detection of aflatoxin B 1 in maize by highly luminescent quantum dot beads [J]. ACS Appl Mater Int , 2014 , 6 ( 16 ): 14215 - 14222 .

XIE Y J , YANG Y , KONG W J , et al . Application of nanoparticle probe-based lateral flow immunochromatographic assay in mycotoxins detection [J]. Chin J Anal Chem , 2015 , 43 ( 4 ): 618 - 628 .

杨英，谢艳君，孔维军，等．基于侧流免疫层析技术制备胶体金试纸条检测莲子中黄曲霉毒素B 1 的研究 [J]. 中南药学， 2015 , 13 ( 3 ): 246 - 250 .

林方芬，郑志勇，黄剑，等．中药饮片中黄曲霉毒素B 1 含量的快速测定研究 [J]. 江苏中医药， 2016 , 48 ( 12 ): 65 - 66,69 .

谢艳君．胶体金试纸条现场可视化检测中药中黄曲霉毒素B 1 研究 [D]．长春：吉林农业大学， 2016 .

武会娟，管笛，亢子佳，等．一种检测黄曲霉毒素B 1 的磁性免疫层析试剂盒及制备方法：中国， CN104459141A [P]. 2015-03-25 .

王艳霞．基于磁性微粒的黄曲霉毒素样本净化及层析试纸条研究 [D]．西安：西北大学， 2012 .

Urusov A E , Zherdev A V , Dzantiev B B. Use of gold nanoparticle-labeled secondary antibodies to improve the sensitivity of an immunochromatographic assay for aflatoxin B 1 [J]. Microchim Acta , 2014 , 181 ( 15/16 ): 1939 - 1946 .

邓省亮，赖卫华，许杨．胶体金免疫层析法快速检测黄曲霉毒素B 1 的研究 [J]. 食品科学， 2007 , 28 ( 2 ): 232 - 236 .

LI X , LI P , ZHANG Q , et al . Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B 1 ，ochratoxin A and zearalenone in agro-food [J]. Biosens Bioelectron , 2013 , 49 ( 22 ): 426 - 432 .

WANG L , WANG S , ZHANG J , et al . Enzyme-linked immunosorbent assay and colloidal gold immunoassay for sulphamethazine residues in edible animal foods: investigation of the effects of the analytical conditions and the sample matrix on assay performance [J]. Anal Bioanal Chem , 2008 , 390 ( 6 ): 1619 - 1627 .

张欣悦．中药材中黄曲霉菌及其毒素检测新技术研究 [D]．北京：北京中医药大学， 2017 .

WANG Z , LI X , SONG Y , et al . An upconversion luminescence nanoprobe for the ultrasensitive detection of hyaluronidase [J]. Anal Chem , 2015 , 87 ( 11 ): 5816 - 5823 .

余宇燕，邱亚利，张红艳，等．直接竞争荧光免疫法测定中药材中的黄曲霉毒素B 1 [J]. 化学通报， 2015 , 78 ( 9 ): 830 - 834 .

ZHANG L , DOU X , ZHANG C , et al . Facile preparation of stable PEG-functionalized quantum dots with glycine-enhanced photoluminescence and their application for screening of aflatoxin B 1 ，in herbs [J]. Sens Actuators B Chem , 2018 , 261 : 188 - 195 .

Beloglazova N V , Goryacheva O A , Speranskaya E S , et al . Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay [J]. Talanta , 2015 , 134 : 120 - 125 .

赵凤英，谢云志，李亿保，等．水溶性稀土上转换纳米颗粒的合成与表征 [J]. 中国稀土学报， 2014 , 32 ( 5 ): 541 - 547 .

SUN C , LI H , Koidis A , et al . Quantifying aflatoxin B 1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles [J]. Spectrochim Acta A , 2016 , 165 : 120 - 126 .

ZHANG Z , LI Y , LI P , et al . Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B 1 in peanuts [J]. Food Chem , 2014 , 146 ( 1 ): 314 - 319 .

DENG G , XU K , SUN Y , et al . High sensitive immunoassay for multiplex mycotoxin detection with photonic crystal microsphere suspension array [J]. Anal Chem , 2013 , 85 ( 5 ): 2833 - 2840 .

Beloglazova N V , Eremin S A. Rapid screening of aflatoxin B 1 in beer by fluorescence polarization immunoassay [J]. Talanta , 2015 , 142 : 170 - 175 .

Adarsha M H , Ramani V M , Madhusudhan B. Development of fluorophore labeled anti-aflatoxin antibodies coated SPIONs for detection of aflatoxin B 1 [J]. J Bionanosci , 2015 , 9 ( 2 ): 94 - 101 .

Kim S , Lim H B. Chemiluminescence immunoassay using magnetic nanoparticles with targeted inhibition for the determination of ochratoxin A [J]. Talanta , 2015 , 140 : 183 - 188 .

陈建魁，左向华，金欣，等．化学发光免疫分析系统质量管理相关问题探讨 [J]. 中国卫生检验杂志， 2011 , 21 ( 3 ): 759 - 760 .

裘雪梅，朱立鑫，刘仁荣，等．黄曲霉毒素B 1 化学发光免疫检测方法的建立 [J]. 畜牧与饲料科学， 2015 , 36 ( 10 ): 29 - 32 .

刘焕春．谷物中黄曲霉素B 1 的间接竞赛争化学发光酶免疫分析方法的研究 [D]．南昌：南昌大学， 2011 .

张良，陈灰，韩盈盈，等．黄曲霉毒素B 1 间接竞争化学发光酶免疫分析方法的建立 [J]. 中国兽医学报， 2013 , 33 ( 3 ): 450 - 457 .

FANG L , CHEN H , YING X , et al . Micro-plate chemiluminescence enzyme immunoassay for aflatoxin B 1 in agricultural products [J]. Talanta , 2011 , 84 ( 1 ): 216 - 262 .

汪玉，徐桂芳，张清榕，等．基于磁性纳米纤维/碳纳米角修饰的黄曲霉毒素电化学发光免疫传感器的研制与应用 [EB/OL]．中国科技论文在线 , 2015 , http://www.paper.edu.cn/releasepaper/content/201509-95 http://www.paper.edu.cn/releasepaper/content/201509-95 .

Malhotra B D , Srivastava S , Ali M A , et al . Nanomaterial-based biosensors for food toxin detection [J]. Appl Biochem Biotech , 2014 , 174 ( 3 ): 880 - 896 .

董国良，彭大鹏，韩肖亚，等．饲料及畜禽产品中霉菌毒素快速检测技术研究进展 [J]. 畜牧兽医学报， 2016 , 47 ( 9 ): 1757 - 1767 .

Adányi N , Levkovets I A , Rodriguez-Gil S , et al . Development of immunosensor based on OWLS technique for determining aflatoxin B 1 and ochratoxin A [J]. Biosens Bioelectron , 2007 , 22 ( 6 ): 797 - 802 .

Rejeb I B , Arduini F , Arvinte A , et al . Development of a bio-electrochemical assay for AFB 1 detection in olive oil [J]. Biosens Bioelectron , 2009 , 24 ( 7 ): 1962 - 1968 .

Majzik A , Hornok V , Sebök D , et al . Sensitive detection of aflatoxin B 1 molecules on gold SPR chip surface using functionalized gold nanoparticles [J]. Cereal Res Commun , 2015 , 43 ( 3 ): 426 - 437 .

张奇，李培武，陈小媚，等．黄曲霉毒素免疫检测技术研究进展 [J]. 农产品质量与安全， 2013 ( 3 ): 42 - 46 .

Srivastava S , Kumar V , Arora K , et al . Antibody conjugated metal nanoparticle decorated graphene sheets for a mycotoxin sensor [J]. RSC Adv , 2016 , 6 ( 61 ): 56518 - 56526 .

LI Q , LV S , LU M , et al . Potentiometric competitive immunoassay for determination of aflatoxin B 1 ，in food by using antibody-labeled gold nanoparticles [J]. Microchim Acta , 2016 , 183 ( 10 ): 2815 - 2822 .

LV X , LI Y , CAO W , et al . A label-free electrochemiluminescence immunosensor based on silver nanoparticle hybridized mesoporous carbon for the detection of aflatoxin B 1 [J]. Sensor Actuators B Chem , 2014 , 202 ( 4 ): 53 - 59 .

WU S , DUAN N , ZHU C , et al . Magnetic nanobead-based immunoassay for the simultaneous detection of aflatoxin B 1 and ochratoxin A using upconversion nanoparticles as multicolor labels [J]. Biosens Bioelectron , 2011 , 30 ( 1 ): 35 - 42 .

Castillo G , Spinella K , Poturnayová A , et al . Detection of aflatoxin B 1 ，by aptamer-based biosensor using PAMAM dendrimers as immobilization platform [J]. Food Control , 2015 , 52 : 9 - 18 .

LI T , Byun J Y , Kim B B , et al . Label-free homogeneous FRET immunoassay for the detection of mycotoxins that utilizes quenching of the intrinsic fluorescence of antibodies [J]. Biosens Bioelectron , 2013 , 42 : 403 - 408 .

鲍蕾，吕宁，吴振兴，等．免疫亲和柱同时净化-HPLC法测定植物油中黄曲霉毒素和玉米赤霉烯酮 [J]. 食品工业科技， 2013 , 34 ( 9 ): 306 - 309 .

GUO X , WEN F , ZHENG N , et al . Development of an ultrasensitive aptasensor for the detection of aflatoxin B 1 [J]. Biosens Bioelectron , 2014 , 56 ( 1 ): 340 - 344 .

谭芸．电化学免疫传感器用于黄曲霉毒素和蛋白质检测的研究 [D]．长沙：湖南大学， 2009 .

王莹．同时检测多种真菌毒素的生物芯片技术研究 [D]．武汉：华中农业大学， 2012 .

王丹．建立同时检测三种真菌毒素膜免疫芯片方法的研究与抗庆大霉素多克隆抗体的制备 [D]．南昌：南昌大学， 2010 .

章先．农产品中四种常见真菌毒素免疫检测技术研究 [D]．杭州：浙江大学， 2016 .

WANG Y , LIU N , NING B , et al . Simultaneous and rapid detection of six different mycotoxins using an immunochip [J]. Biosens Bioelectron , 2012 , 34 ( 1 ): 44 - 50 .

邓国哲．基于三维光子晶体微球流式芯片的真菌毒素高通量检测技术研究 [D]．南京：南京师范大学， 2012 .

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黄曲霉毒素B1免疫快速检测技术研究进展及其在中药中的应用

Rapid Immunoassay of Aflatoxin B1 and Its Application in Traditional Chinese Medicine

DOI：10.13422/j.cnki.syfjx.20190811

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黄曲霉毒素B₁免疫快速检测技术研究进展及其在中药中的应用

Rapid Immunoassay of Aflatoxin B₁ and Its Application in Traditional Chinese Medicine