Objective:

2

To establish an HPLC method for simultaneous determination of geniposide

chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

isochlorogenic acid A

B and C in Qinggan Lidan mixture

in order to provide references for its quality control.

Method:

2

The analysis of methanol extract of this drug was performed on a 35 ℃ Luna C

18

column (4.6 mm×250 mm

5μm)

with the mobile phase comprised of acetonitrile-0.4%phosphoric acid flowing at 1.0 mL·min

-1

in a gradient elution mode (0-10 min

8%-12%A; 10-30 min

12%A; 30-60 min

12%-35%A)

and the detection wavelengths were set at 238 and 327 nm.

Result:

2

Geniposide

chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

isochlorogenic acid A

B and C were completely separated

and well separated from other constituents. The linear ranges of geniposide

chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

isochlorogenic acid A

B and C were 0.188-2.355

0.083-1.040

0.074-0.920

0.075-0.940

0.064-0.800

0.076-0.955

0.071-0.888 μg (

r

≥0.999 0)

respectively. The average recoveries were 99.45%

98.45%

99.06%

98.50%

98.16%

101.01%

96.93%

with the RSDs of 0.5%

1.8%

1.3%

2.4%

2.3%

1.6%

1.6%

respectively.The contents of geniposide

chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

isochlorogenic acid A

B and C were 3.420-3.794

0.835-0.890

1.222-1.275

1.064-1.210

0.377-0.398

0.419-0.464 and 0.362-0.405 g·L

-1

respectively.

Conclusion:

2

This method can be used for simultaneous determination of muti-ingredients in Qinggan Lidan mixture

and the established method is simple and accurate

with a good reproducibility and high sensitivity. It can be used for the quality control of Qinggan Lidan mixture.