Objective:

2

To establish HPLC-UV fingerprints of

Ilex pubescens

pieces

and simultaneously determine two components in 46 batches of

I

.

pubescens

in pieces of

I

.

pubescens

saponin A

1

and B

1

in order to provide a reference for the quality standard of

I

.

pubescens

slices.

Method:

2

Methanol was used to extract the

I

.

pubescens

saponin samples

and the extracts were measured by HPLC-UV with the absorption wavelength at 210 nm. Kromasil C

18

column (4.6 mm×250 mm

5 μm) was used for determining the extracts at a flow rate of 1.0 mL·min

-1

. The mobile phase condition was acetonitrile-0.1%phosphoric acid aqueous solution with gradient mode. The chromatographic fingerprint similarity evaluation system of traditional Chinese medicine (2012 edition) was used to analyze

I

.

pubescens

fingerprints. SPSS 20.0 software was used to cluster the peak area of common peaks. Principal component analysis was performed to reduce the dimension of common peaks.

Result:

2

There were great differences between the root and stem parts in

I

.

pubescens

fingerprints. The fingerprints of roots and stems of

I

.

pubescens

were established respectively

cluster results assorted the roots of

I

.

pubescens

into three categories andthe branches of

I

.

pubescens

into two categories. The integrity and difference of

I

.

pubescens

decoction pieces from different parts and places of origin were compared

and the principal component analysis was performed to screen out the common components that played a decisive role in fingerprint of

I

.

pubescens

pieces. And the common peaks were determined. The content of saponin A

1

and saponin B

1

in Radix

I

.

pubescens

were determined.

Conclusion:

2

The established

I

.

pubescens

fingerprints and content determination methods are simple and suitable. Cluster analysis and principal component analysis are used to screen out the key components of quality control of

I

.

pubescens

. The results can provide references for quality control of

I

.

pubescens

.