人参-知母-赤芍提取物对血管性痴呆大鼠海马神经元的保护作用及机制

郭洋洋; 韩振蕴; 田丹枫; 王东辉; 于雪; 郭婕; 张丹丹; 潘彦舒

doi:10.13422/j.cnki.syfjx.20190837

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人参-知母-赤芍提取物对血管性痴呆大鼠海马神经元的保护作用及机制

药理 | 更新时间：2021-02-09

- 人参-知母-赤芍提取物对血管性痴呆大鼠海马神经元的保护作用及机制
- Protective Effect and Mechanism of Extracts from Ginseng Radix et Rhizoma,Anemarrhenae Rhizoma and Paeoniae Radix Rubra on Hippocampal Neurons in Rats with Vascular Dementia
- 中国实验方剂学杂志 2019年25卷第8期页码：47-53
- 作者机构：
  
  1.北京中医药大学　中医学院，北京　 100029；
  2.北京中医药大学　东方医院，北京　 100078
- 作者简介：
  
  郭洋洋，在读硕士，从事脑血管病的中西医防治研究，E-mail: guoyangyang39@163.com
  潘彦舒，博士，教授，从事脑血管病的中西医防治研究，E-mail: 13911209998@163.com
- 基金信息：
  
  国家自然科学基金面上项目(8167150452);西藏自治区藏医药区域协同创新中心第一批培育项目(2017XTCX014)
- DOI：10.13422/j.cnki.syfjx.20190837
  中图分类号： R285.5;R331.3;R322.81;R743
- 收稿日期：2018-11-08，
  
  网络出版日期：2019-01-04，
  
  纸质出版日期：2019-04-20
- 稿件说明：
移动端阅览
郭洋洋, 韩振蕴, 田丹枫, 等. 人参-知母-赤芍提取物对血管性痴呆大鼠海马神经元的保护作用及机制[J]. 中国实验方剂学杂志, 2019,25(8):47-53.

Yang-yang GUO, Zhen-yun HAN, Dan-feng TIAN, et al. Protective Effect and Mechanism of Extracts from Ginseng Radix et Rhizoma,Anemarrhenae Rhizoma and Paeoniae Radix Rubra on Hippocampal Neurons in Rats with Vascular Dementia[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 47-53.
郭洋洋, 韩振蕴, 田丹枫, 等. 人参-知母-赤芍提取物对血管性痴呆大鼠海马神经元的保护作用及机制[J]. 中国实验方剂学杂志, 2019,25(8):47-53. DOI： 10.13422/j.cnki.syfjx.20190837.

Yang-yang GUO, Zhen-yun HAN, Dan-feng TIAN, et al. Protective Effect and Mechanism of Extracts from Ginseng Radix et Rhizoma,Anemarrhenae Rhizoma and Paeoniae Radix Rubra on Hippocampal Neurons in Rats with Vascular Dementia[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 47-53. DOI： 10.13422/j.cnki.syfjx.20190837.

摘要

目的：

观察人参-知母-赤芍提取物对血管性痴呆大鼠海马

-甲基-

-天冬氨酸受体1(NMDAR1)的影响，探讨其保护海马神经元的作用机制。

方法：

60只SPF级雄性SD大鼠，随机分为正常组，假手术组，模型组，人参-知母-赤芍提取物组(0.20 g·kg

-1

)和美金刚组(2.1 mg·kg

-1

)，每组12只。采用双侧颈总动脉反复夹闭合并腹腔注射硝普钠的方法建立血管性痴呆大鼠模型，造模后，正常组，假手术组，模型组给予同等体积生理盐水，每日1次，连续14 d。采用Morris水迷宫评价各组大鼠学习记忆能力；苏木素-伊红(HE)染色观察海马CA1区病理改变；蛋白免疫印迹法(Western blot)检测海马神经元胞膜NMDAR1的蛋白表达水平；免疫组化法(IHC)检测海马NMDAR1的表达情况；实时荧光定量PCR(Real-time PCR)检测海马组织中NMDAR1 mRNA的表达水平。

结果：

与正常组和假手术组比较，模型组大鼠逃避潜伏期显著延长(

0.01)，在平台所在象限停留时间及穿越平台的次数显著减少(

0.01)，海马CA1区神经细胞层次减少，排列紊乱、出现核固缩、神经元丢失，NMDAR1蛋白及mRNA表达显著升高(

0.01)；与模型组比较，人参-知母-赤芍提取物组，美金刚组逃避潜伏期明显缩短(

0.05，

0.01)，在平台所在象限停留时间及穿越平台的次数明显增加(

0.05，

0.01)，海马CA1区神经元数目与形态有明显改善，海马神经元NMDAR1蛋白及NMDAR1 mRNA表达明显降低(

0.05)。

结论：

人参-知母-赤芍提取物能够改善血管性痴呆大鼠的学习记忆能力，减轻海马CA1区神经元的损伤，其机制可能与下调海马神经元NMDAR1的表达有关。

Abstract

Objective:

To observe the effect of extracts from Ginseng Radix et Rhizoma

Anemarrhenae Rhizoma and Paeoniae Radix Rubra on

-methyl-

-aspartate receptors(NMDAR1) in hippocampal neurons in rats with vascular dementia and investigate its possible mechanism.

Method:

The 60 SPF male rats were randomly divided into normal group

sham-operated group

model group

traditional Chinese medicine group(0.20 g·kg

-1

)and memantine group(2.1 mg·kg

-1

)

with 12 rats in each group. The model was established by repeated ischemia-reperfusion combined with intraperitoneal injection of sodium nitroprusside. After modelling

normal group

sham-operated group and model group were dosed the similar volume of normal saline once a day for 14 days. The learning and memory capacity was assessed by Morris water maze; pathologic change in the CA1 district of hippocampus was assessed by hematoxylin-eosin (HE) staining

and the expression level of NMDAR1 in hippocampal neuron membrane protein was detected by Western blot and immunohistochemistry(IHC)

the NMDAR1 mRNA in hippocampal tissue was detected by Real-time PCR.

Result:

Compared with normal and sham-operated group

the latency period was prolonged in model group(

0.01)

the time in the platform quadrant and the frequency of crossing the platform were lessened significantly(

0.01)

the disorder of the neurons

the decrease of the neuronal number

and the neuronal necrosis and apoptosis were obversed in the CA1 district

expression of membrane NMDAR1 of the hippocampal neuron and NMDAR1 mRNA were increased significantly(

0.01). Compared with model group

the latency period of extracts from Ginseng Radix et Rhizoma

Anemarrhenae Rhizoma and Paeoniae Radix Rubra group and memantine group were shortened significantly(

0.05

0.01)

the time and frequency were increased(

0.05

0.01)

the pathologic change was improved markedly

the protein expression of membrane NMDAR1 in hippocampal neuron and NMDAR1 mRNA were decreased significantly(

0.01).

Conclusion:

The extracts from Ginseng Radix et Rhizoma

Anemarrhenae Rhizoma and Paeoniae Radix Rubra can improve the learning and memory capacity of rats with vascular dementia

and alleviate the injury in CA1 district of hippocampus. The mechanism may be related to the down-regulation of NMDAR1 expression in hippocampal neurons.

关键词

Keywords

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