EV71和CA16型手足口病动物模型及其病毒感染特点分析

姚荣妹; 毛鑫; 曲天歌; 郭姗姗; 包蕾; 鮑岩岩; 崔晓兰

doi:10.13422/j.cnki.syfjx.20190841

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EV71和CA16型手足口病动物模型及其病毒感染特点分析

综述 | 更新时间：2021-02-09

- EV71和CA16型手足口病动物模型及其病毒感染特点分析
- Animal Models of EV71 and CA16 Hand-foot-mouth Disease and Characteristics of Viral Infection
- 中国实验方剂学杂志 2019年25卷第8期页码：172-180
- 作者机构：
  
  1.中国中医科学院　中药研究所，北京　 100700；
  2.华北理工大学　中医学院，河北　唐山　 063210；
  3.北京中医药大学　基础医学院，北京　 100029
- 作者简介：
  
  姚荣妹，在读博士，讲师，从事中药抗菌及抗病毒药效评价及机制研究，E-mail: 471268167@qq.com
  崔晓兰，研究员，博士生导师，从事中药抗菌及抗病毒药效评价及机制研究，E-mail: cuixiaolan2812@126.com
- 基金信息：
  
  国家“重大新药创制”科技重大专项(2011ZDXK-03)
- DOI：10.13422/j.cnki.syfjx.20190841
  中图分类号： G353.11;R373.2;R272
- 收稿日期：2018-10-08，
  
  网络出版日期：2019-01-04，
  
  纸质出版日期：2019-04-20
- 稿件说明：
移动端阅览
姚荣妹, 毛鑫, 曲天歌, 等. EV71和CA16型手足口病动物模型及其病毒感染特点分析[J]. 中国实验方剂学杂志, 2019,25(8):172-180.

Rong-mei YAO, Xin MAO, Tian-ge QU, et al. Animal Models of EV71 and CA16 Hand-foot-mouth Disease and Characteristics of Viral Infection[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 172-180.
姚荣妹, 毛鑫, 曲天歌, 等. EV71和CA16型手足口病动物模型及其病毒感染特点分析[J]. 中国实验方剂学杂志, 2019,25(8):172-180. DOI： 10.13422/j.cnki.syfjx.20190841.

Rong-mei YAO, Xin MAO, Tian-ge QU, et al. Animal Models of EV71 and CA16 Hand-foot-mouth Disease and Characteristics of Viral Infection[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(8): 172-180. DOI： 10.13422/j.cnki.syfjx.20190841.

摘要

手足口病(HFMD)是由肠道病毒引起的儿童常见传染病，发病率高，可引发肺水肿、心肌炎、无菌性脑膜炎等致命性并发症，严重威胁着患儿的健康。目前该病相关病毒的致病机制、致病病毒不同基因型之间的关系、药效学评价方法、药物抗病毒机制等核心问题尚不明确，而构建具有人类染毒模拟表现的动物模型是解决以上问题的关键。国内外研究人员建立了多种不同动物种属、品系的HFMD动物模型，以肠道病毒71型(EV71)和柯萨奇病毒A16型(CA16)最常见，应用也最为广泛。EV71与CA16同属小核糖核酸病毒科肠道病毒属肠道病毒A型，两者在病原学、感染和复制特征、感染导致的临床症状和免疫反应有相似的方面，但引发的HFMD动物模型在易感年龄，感染方法，表现出的神经毒性、临床症状及体征、组织器官损伤程度等也有明显不同的方面，因此，研究者应根据实际需要选择和建立合适的动物模型，这对于研究结果的可靠性具有重要意义。本文通过梳理感染EV71和CA16病毒建立的不同物种HFMD动物模型，分类阐述各模型的种属品系、病毒株类型、感染方法、病毒感染特点，深入研究EV71及CA16感染诱发的HFMD特点及其感染所导致的临床症状差异，整体评价小鼠、树鼩及非人灵长类3类动物模型各自的优缺点，以期为相关研究提供参考。

Abstract

Hand-foot-mouth disease (HFMD) is a common infectious disease caused by enterovirus in children. It has a high incidence and can cause fatal complications such as pulmonary edema

myocarditis and aseptic meningitis

seriously threatening the health of children. At present

some core problems such as the pathogenesis of disease

the relationship between different genotypes of pathogenic viruses

the pharmacodynamic evaluation methods

and the antiviral mechanism of drugs are still unclear. The construction of disease animal models with simulation performance of human exposure is the key to solve the above problems. Researchers both at home and abroad have established a variety of animal models for HFMD

of which enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are most common and most widely used. Both EV71 and CA16 are enterovirus A in picornavirus family

so they have similarities in terms of pathogenicity

infection and replication characteristics

clinical symptoms caused by infection and immune response

but also have significant differences in age of susceptibility

method of infection

as well as neurotoxicity

clinical symptoms and signs

and degree of tissue and organ damage. Therefore

researchers shall select and establish proper animal models based on actual conditions

which is critical to the reliability of the results. In this paper

the different types of HFMD animal models established by EV71 and CA16 viruses were reviewed

especially on the species strains

virus strain types

infection methods

and characteristics of viral infections in each model

and the characteristics and clinical symptoms of HFMD induced by EV71 and CA16 were also investigated to provide reference for related research.

关键词

Keywords

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