白头翁汤对溃疡性结肠炎大鼠的疗效及免疫机制的影响

钟宇; 郑学宝; 叶华; 郭蒙; 吴琼; 迟宏罡; 邹颖; 朱宇珍

doi:10.13422/j.cnki.syfjx.20190903

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白头翁汤对溃疡性结肠炎大鼠的疗效及免疫机制的影响

经典名方 | 更新时间：2021-02-09

- 白头翁汤对溃疡性结肠炎大鼠的疗效及免疫机制的影响
- Efficacy and Immune Mechanism of Baitouweng Tang on Ulcerative Colitis in Rats
- 中国实验方剂学杂志 2019年25卷第12期页码：15-21
- 作者机构：
  
  1.广东医科大学，广东　湛江　 524023；
  2.广州中医药大学，广州　 510006
- 作者简介：
  
  钟宇，硕士，从事中药药理学的研究，Tel：0759-2388591，E-mail: zhongyu517618@163.com
  郑学宝，博士，教授，从事中药复方治疗消化道疾病的实验与临床，Tel：0759-2388405，E-mail: xuebaozhenggzy@126.com；
  朱宇珍，博士，从事中药复方治疗消化道疾病的实验与临床，Tel：0759-2388405，E-mail: zhuyuzhen2003@126.com
- 基金信息：
  
  国家自然科学基金项目(81573932;81603592);东莞市社会科技发展（重点）项目(20185071501131614);广东省医学科研基金项目(A2017005;B2017044);广东省中医药局项目(20182072)
- DOI：10.13422/j.cnki.syfjx.20190903
  中图分类号： R2-0; R22; R285.5; R289
- 收稿日期：2018-10-05，
  
  网络出版日期：2019-01-18，
  
  纸质出版日期：2019-06-20
- 稿件说明：
移动端阅览
钟宇, 郑学宝, 叶华, 等. 白头翁汤对溃疡性结肠炎大鼠的疗效及免疫机制的影响[J]. 中国实验方剂学杂志, 2019,25(12):15-21.

Yu ZHONG, Xue-bao ZHENG, Hua YE, et al. Efficacy and Immune Mechanism of Baitouweng Tang on Ulcerative Colitis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 15-21.
钟宇, 郑学宝, 叶华, 等. 白头翁汤对溃疡性结肠炎大鼠的疗效及免疫机制的影响[J]. 中国实验方剂学杂志, 2019,25(12):15-21. DOI： 10.13422/j.cnki.syfjx.20190903.

Yu ZHONG, Xue-bao ZHENG, Hua YE, et al. Efficacy and Immune Mechanism of Baitouweng Tang on Ulcerative Colitis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 15-21. DOI： 10.13422/j.cnki.syfjx.20190903.

摘要

目的：

探讨白头翁汤对溃疡性结肠炎(UC)大鼠的治疗效果及免疫机制。

方法：

采用2，4-二硝基氯苯(DNCB)加醋酸/乙醇复合法建立UC大鼠模型，将造模成功的大鼠随机分成模型组、美沙拉嗪组和白头翁汤高、中、低剂量组，正常大鼠为对照组。美沙拉嗪组给予美沙拉嗪(0.5 g·kg

－1

)灌胃，白头翁汤高、中、低剂量组分别给予白头翁汤(10，5，2.5 g·kg

－1

)灌胃，其余各组给予双蒸水。连续给药7 d后，观察各组大鼠一般情况和疾病活动指数；大鼠麻醉后腹主动脉取血，处死大鼠，取结肠测长度，肉眼观察结肠黏膜损伤程度及大体评分；光镜下观察结肠组织病理形态学变化及病理评分；采用分光光度计比色法检测血清和结肠组织匀浆液中性粒细胞髓过氧化物酶(MPO)活性；酶联免疫吸附测定(ELISA)法测定血清和结肠组织匀浆液P-选择素、巨噬细胞移动抑制因子(MIF)和血栓素B

(TXB

)水平；免疫组织化学法及蛋白免疫印迹法(Western blot)技术检测结肠组织Toll样受体4(TLR4)和核转录因子-

B(NF-

B)蛋白表达。

结果：

与正常组比较，模型组出现稀便、腹泻及血便等症状，DAI，结肠大体评分及病理学评分显著增高(

0.01)；白头翁汤组明显改善UC症状，DAI，结肠大体形态及病理学评分显著下降(

0.01)。模型组血清和结肠组织中MPO，P-选择素，MIF和TXB

的含量较正常组显著升高；白头翁汤高、中剂量组能显著降低MPO，P-选择素，MIF和TXB

的水平。模型组结肠组织中TLR4和NF-

B蛋白表达量均显著高于正常组；高、中、低剂量组均能明显抑制TLR4和NF-

B的表达。

结论：

白头翁汤可能通过抑制TLR4/NF-

B信号通路，下调P-选择素，MPO，MIF，TXB

的水平，促进肠道黏膜的的修复，减轻结肠炎症反应。

Abstract

Objective:

To investigate the therapeutic effect and immune mechanism of Baitouweng Tang on ulcerative colitis(UC) rats.

Method:

The mode of UC rats was made of 2

4-dinitrochlorobenzene (DNCB)/ethanol enema. Rats were randomly divided into control group

model group

mesalazine group

and high-dose

middle-dose and low-dose Baitouweng Tang groups. The mesalazine group were administered with mesalazine (0.5 g·kg

-1

). Baitouweng Tang groups were given Baitouweng Tang (10

2.5 g·kg

-1

)

while the other groups were given double steaming water. After 7 days of continuous administration

the general condition and disease activity index of rats in each group were observed. After anesthesia in rats

blood was taken from the abdominal aorta. Then the rats were put to death

and the length and morphological observation of the colon were measured. Ultraviolet spectrophotometry detection was used to detect the activities of myeloperoxidase (MPO) in blood and colon tissue. The levels of P-selectin

macrophage migration inhibitory factor (MIF) and thromboxane B

(TXB

) in blood and colon tissues were detected by enzyme-linked immunosorbent assay(ELISA). Immunohistochemistry and Western blot methods were undertaken to determine the expressions of Toll-like receptor 4 (TLR4) and nuclear transcription factor-

B (NF-

B) proteins in colon tissue.

Result:

Compared with the model group

the rats in model group showed severe symptoms

such as loose stools

diarrhea and bloody stools

while Baitouweng Tang obviously ameliorated them. Moreover

Baitouweng Tang significantly reduced DAI

colon general and pathological scores

which were high in model group(

0.01). The levels of MPO

P-selectin

MIF and TXB

in the serum and colon tissues of the model group were obviously increased(

0.01)

and Baitouweng Tang could reverse them. Similarly

the expressions of TLR4 and NF-

B in colons of model group were markedly higher than those in control group(

0.01). However

Baitouweng Tang group showed lower expressions than the model group (

0.01).

Conclusion:

Baitouweng Tang could inhibit TLR4/NF-

B signaling pathway in treatment of ulcerative colitis

and reduce the expressions of P-selectin

MPO

MIF and TXB

and thus promoting intestinal mucosal repair and improving intestinal function.

关键词

Keywords

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