肠激安方对IBS-D大鼠肠道通透性的影响

石玉莹; 唐洪梅; 吴映秀; 祝赫; 何国栋; 熊芬; 张毅靖

doi:10.13422/j.cnki.syfjx.20190905

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肠激安方对IBS-D大鼠肠道通透性的影响

药理 | 更新时间：2021-02-09

- 肠激安方对IBS-D大鼠肠道通透性的影响
- Effect of Changji′an Prescription on Intestinal Permeability of IBS-D Rats
- 中国实验方剂学杂志 2019年25卷第11期页码：80-85
- 作者机构：
  
  1.广州中医药大学　岭南医学研究中心，广州　 510405；
  2.广州中医药大学　第一附属医院，广州　 510405
- 作者简介：
  
  石玉莹，在读硕士，从事中药新药开发与安全性评价研究，E-mail：389669268 @qq.com
  唐洪梅，博士，教授，主任中药师，从事中药新药开发与安全性评价研究，Tel：020-86358342，E-mail：tanghongmei2000@163.com
- 基金信息：
  
  国家自然科学基金项目(81673842);广东省自然科学基金项目(2014A030313404)
- DOI：10.13422/j.cnki.syfjx.20190905
  中图分类号： R2-0; R22; R285.5; R289
- 收稿日期：2018-09-26，
  
  网络出版日期：2019-01-18，
  
  纸质出版日期：2019-06-05
- 稿件说明：
移动端阅览
石玉莹, 唐洪梅, 吴映秀, 等. 肠激安方对IBS-D大鼠肠道通透性的影响[J]. 中国实验方剂学杂志, 2019,25(11):80-85.

Yu-ying SHI, Hong-mei TANG, Ying-xiu WU, et al. Effect of Changji′an Prescription on Intestinal Permeability of IBS-D Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 80-85.
石玉莹, 唐洪梅, 吴映秀, 等. 肠激安方对IBS-D大鼠肠道通透性的影响[J]. 中国实验方剂学杂志, 2019,25(11):80-85. DOI： 10.13422/j.cnki.syfjx.20190905.

Yu-ying SHI, Hong-mei TANG, Ying-xiu WU, et al. Effect of Changji′an Prescription on Intestinal Permeability of IBS-D Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 80-85. DOI： 10.13422/j.cnki.syfjx.20190905.

摘要

目的：

观察肠激安方对腹泻型肠易激综合征(IBS-D)大鼠肠道通透性的影响，探讨肠激安方治疗IBS-D的作用机制。

方法：

SD雄性乳鼠，随机分为5组，分别为正常组，模型组，匹维溴铵组(0.018 g·kg

－1

)，肠激安高、低剂量组(33.48，16.74 g·kg

－1

)，除正常组外，均采用“母婴分离＋醋酸刺激＋束缚应激”三因素结合的方法建立IBS-D模型。给药后，采用透射电镜观察大鼠肠黏膜超微结构；酶联免疫吸附测定(ELISA)法检测大鼠血浆

-乳酸水平；蛋白免疫印迹法(Western blot)检测大鼠结肠紧密连接蛋白闭锁蛋白(Occludin)，闭合蛋白-1(Claudin-1)的表达；实时荧光定量聚合酶链式反应(Real-time PCR)检测Occludin，Claudin-1，紧密连接蛋白-1(ZO-1) mRNA表达。

结果：

与正常组比较，IBS-D模型组大鼠肠黏膜上皮细胞损伤，微绒毛排列较稀疏，紧密连接间隙增宽，有的不甚明显；血浆

-乳酸水平明显升高(

0.05)，结肠中Occludin，Claudin-1 mRNA及蛋白表达降低(

0.05)，ZO-1 mRNA表达降低(

0.05)。药物治疗后，与模型组比较，各给药组大鼠肠黏膜细胞损伤修复；匹维溴铵组、肠激安方高剂量组血浆

-乳酸水平明显降低(

0.05)，肠激安方低剂量组血浆

-乳酸水平有降低趋势，但不具有统计学差异。各给药组大鼠结肠中Occludin，Claudin-1 mRNA及蛋白表达水平，ZO-1 mRNA表达水平升高(

0.05)。

结论：

肠激安方对IBS-D的治疗作用可能是改善肠道通透性实现的。

Abstract

Objective:

To observe the effect of Changji′an prescription on intestinal permeability in diarrhea-predominant irritable bowel syndrome (IBS-D) rats and explore its mechanism for treatment of IBS-D.

Method:

Male SD neonatal rats were randomly divided into five groups: normal group

model group

pinaverium bromide group(0.018 g·kg

-1

)

high-dose(33.48 g·kg

-1

) and low-dose (16.74 g·kg

-1

)Changji′an prescription groups. Except for the normal group

the IBS-D model was established by the combination of maternal and infant separation+ acetic acid stimulation+ restraint stress. After drug treatment

the ultrastructure of rat intestinal mucosa was observed by using transmission electron microscopy and the plasma

-lactate level was detected by enzyme-linked immunosorbent assay (ELISA). The expression levels of tight junction proteins Occludin and Claudin-1 were detected by Western blot. The mRNA expression levels of Occludin

Claudin-1 and zonula occluden(ZO)-1 were detected by real time polymerase chain reaction (Real-time PCR).

Result:

As compared with the normal group

the intestinal mucosal epithelial cells were damaged in IBS-D model group

and the microvilli arrangement was sparse and tight junction was widened

and some were not obvious

and the plasma

-lactate level in IBS-D rats was increased significantly (

0.05)

and the mRNA and protein expression levels of Occludin and Claudin-1 in the colon were decreased and the mRNA expression of ZO-1 was also decreased (

0.05). After drug treatment

as compared with the model group

all drug-administered groups can repair intestinal mucosal cell damage. Plasma

-lactate level in pinaverium bromide group and high-dose Changji′an prescription group was significantly decreased (

0.05) while

-lactate level in the low-dose group Changji′an prescription group had a tendency to decrease with no statistical difference. The mRNA and protein expression levels of Occludin and Claudin-1 and the mRNA expression of ZO-1 in the colon of rats in each administration group were higher than those in the model group (

0.05).

Conclusion:

The therapeutic effect of Changji′an prescription on IBS-D may be achieved by improving the intestinal permeability.

关键词

Keywords

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