基于HPLC-Q-TOF-MS/MS定性分析当归中多种香豆素类成分

蒋虎刚; 赵信科; 蔺文燕; 李亮; 李娇; 李应东

doi:10.13422/j.cnki.syfjx.20190918

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基于HPLC-Q-TOF-MS/MS定性分析当归中多种香豆素类成分

资源与质量评价 | 更新时间：2021-02-09

- 基于HPLC-Q-TOF-MS/MS定性分析当归中多种香豆素类成分
- Qualitative Analysis of Multiple Cumarins in Angelicae Sinensis Radix Based on HPLC-Q-TOF-MS/MS
- 中国实验方剂学杂志 2019年25卷第13期页码：157-162
- 作者机构：
  
  1.兰州大学，兰州　 730000；
  2.甘肃中医药大学，兰州　 730000
- 作者简介：
  
  蒋虎刚，硕士，住院医师，从事心血管临床及科研工作，E-mail：980428279@qq.com
  李应东，博士，教授、主任医师，从事心血管临床及科研工作，E-mail：lydj412@163.com
- 基金信息：
  
  甘肃省自然科学基金项目(1508RJZA015)
- DOI：10.13422/j.cnki.syfjx.20190918
  中图分类号： R284.1; R289; R22; R2-031
- 收稿日期：2018-12-30，
  
  网络出版日期：2019-01-18，
  
  纸质出版日期：2019-07-05
- 稿件说明：
移动端阅览
蒋虎刚, 赵信科, 蔺文燕, 等. 基于HPLC-Q-TOF-MS/MS定性分析当归中多种香豆素类成分[J]. 中国实验方剂学杂志, 2019,25(13):157-162.

Hu-gang JIANG, Xin-ke ZHAO, Wen-yan LIN, et al. Qualitative Analysis of Multiple Cumarins in Angelicae Sinensis Radix Based on HPLC-Q-TOF-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 157-162.
蒋虎刚, 赵信科, 蔺文燕, 等. 基于HPLC-Q-TOF-MS/MS定性分析当归中多种香豆素类成分[J]. 中国实验方剂学杂志, 2019,25(13):157-162. DOI： 10.13422/j.cnki.syfjx.20190918.

Hu-gang JIANG, Xin-ke ZHAO, Wen-yan LIN, et al. Qualitative Analysis of Multiple Cumarins in Angelicae Sinensis Radix Based on HPLC-Q-TOF-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 157-162. DOI： 10.13422/j.cnki.syfjx.20190918.

摘要

目的：

建立高效、稳定的HPLC-Q-TOF-MS/MS方法，系统、全面地定性分析当归中多种香豆素成分，为当归质控标准的完善、香豆素的开发及临床应用提供依据。

方法：

通过调整液相色谱柱种类、温度、流动相、流速、样品浓度等条件，建立可高效分离当归提取液中香豆素成分的HPLC-Q-TOF-MS/MS，并结合对照品、精确相对分子质量、极性、裂解规律、参考文献等鉴定当归中多种香豆素类成分。

结果：

该研究建立了一种高效、稳定的香豆素分离方法，并鉴定出14种香豆素成分，发现其存在大量的同分异构体，其中珊瑚菜素、王草酚等香豆素成分较少作为当归成分被报道；同时通过分析香豆素类成分的主要碎片离子，发现香豆素类成分在质谱中主要通过母核侧链的甲氧基键或苯甲醚键的断裂以及侧链上其他基团的丢失而裂解，总结出香豆素成分的一般裂解规律。

结论：

当归中含有大量的香豆素类成分，将其深入定性、定量分析可进一步完善当归的质量标准，并为香豆素的开发及当归的临床应用提供参考。

Abstract

Objective:

To systematically and comprehensively analyze coumarin components in Angelicae Sihensis Radix by an efficient and stable HPLC-Q-TOF-MS/MS method

in order to offer the theoretical basis to develop coumarin

establish the quality control standard and apply in clinic.

Method:

The separation effect of coumarin components was extracted by adjusting the column

temperature

mobile phase

flow rate

sample concentration and other conditions

and various coumarin components in Angelicae Sihensis Radix were identified by corresponding standards

precise molecular mass

polarity

pyrolysis pattern.

Result:

In this study

a high-efficiency and stable coumarin separation method was established that can be used to separate complex components

and 14 coumarin components were identified in this study

including phellopterin and osthenol that were rarely reported as effective components in Angelicae Sihensis Radix. Major fragment ions of coumarin components were analyzed. The cleavage in methoxy bond or anisole bond on the parent nucleus was the primary pattern for coumarin components

which was summarized for detecting unknowing coumarins.

Conclusion:

Abundant coumarins were contained in Angelicae Sihensis Radix. Further qualitative and quantitative analysis of coumarins are conducive to improving the quality standards of Angelicae Sihensis Radix

and providing reference for the development of coumarins and clinical application of Angelicae Sinensis Radix.

关键词

Keywords

references

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