芍药汤对溃疡性结肠炎大鼠肠黏膜免疫屏障的干预作用

吴东升; 曹晖; 张彧; 高亚; 宾东华

doi:10.13422/j.cnki.syfjx.20190936

您当前的位置：

首页 >

文章列表页 >

芍药汤对溃疡性结肠炎大鼠肠黏膜免疫屏障的干预作用

经典名方 | 更新时间：2021-02-09

- 芍药汤对溃疡性结肠炎大鼠肠黏膜免疫屏障的干预作用
- Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis
- 中国实验方剂学杂志 2019年25卷第9期页码：6-11
- 作者机构：
  
  1.湖南中医药大学，长沙　 410208
  2.湖南中医药大学　第一附属医院，长沙　 410007
- 作者简介：
  
  吴东升，在读硕士，从事肠道慢性疾病合并肛周病变的中医药研究，E-mail：987909401@qq.com
  曹晖，副教授，硕士生导师，从事肠道慢性疾病合并肛周病变的研究，E-mail：caohui001818@sina.com
- 基金信息：
  
  国家自然科学基金项目(81603634);湖南省中医药科研计划重点项目(201804);湖南省卫生计生科研计划项目(C20180627);湖南中医药大学校级科研基金项目(ZYYDX201733)
- DOI：10.13422/j.cnki.syfjx.20190936
  中图分类号： R289; R318.14; R516.1; R364.7; R285.5
- 收稿日期：2018-11-03，
  
  网络出版日期：2019-01-18，
  
  纸质出版日期：2019-05-05
- 稿件说明：
移动端阅览
吴东升, 曹晖, 张彧, 等. 芍药汤对溃疡性结肠炎大鼠肠黏膜免疫屏障的干预作用[J]. 中国实验方剂学杂志, 2019,25(9):6-11.

Dong-sheng WU, Hui CAO, Yu ZHANG, et al. Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 6-11.
吴东升, 曹晖, 张彧, 等. 芍药汤对溃疡性结肠炎大鼠肠黏膜免疫屏障的干预作用[J]. 中国实验方剂学杂志, 2019,25(9):6-11. DOI： 10.13422/j.cnki.syfjx.20190936.

Dong-sheng WU, Hui CAO, Yu ZHANG, et al. Intervention Effect of Shaoyaotang on Intestinal Mucosal Immune Barrier in Rats with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(9): 6-11. DOI： 10.13422/j.cnki.syfjx.20190936.

摘要

目的：

探讨芍药汤对2，4，6-三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎大鼠肠黏膜免疫屏障的影响。

方法：

将60只SD大鼠随机分为正常组，模型组，美沙拉嗪组(0.067 g·kg

－1

)，芍药汤低、中、高剂量组(1.8，3.6，7.2 g·kg

－1

)，采用TNBS诱导溃疡性结肠炎模型，对应给予生理盐水、美沙拉嗪、芍药汤灌胃治疗7 d，采用苏木素-伊红(HE)染色观察结肠组织病理学改变，免疫组化法和蛋白免疫印迹法(Western blot)检测肠黏膜中CD4

＋

T淋巴细胞数量和分泌型免疫球蛋白A(SIgA)蛋白的表达。

结果：

与正常组比较，模型组大鼠肠黏膜损伤程度评分及病理学评分显著升高(

0.01)；CD4

＋

T淋巴细胞，SIgA蛋白表达显著下降(

0.01)；与模型组比较，各给药组大鼠结肠黏膜损伤程度评分及病理学评分显著下降(

0.01)，肠黏膜CD4

＋

T淋巴细胞，SIgA表达明显升高(

0.05，

0.01)；与美沙拉嗪组及芍药汤低剂量组比较，芍药汤中、高剂量组肠黏膜损伤程度评分和病理学评分显著下降(

0.01)，芍药汤中、高剂量组大鼠肠黏膜中CD4

＋

T淋巴细胞及SIgA蛋白表达明显升高(

0.05)。

结论：

芍药汤可通过增加肠黏膜中CD4

＋

T细胞数量及SIgA分泌的表达，起到减轻肠黏膜损伤，保护肠黏膜免疫屏障功能作用。

Abstract

Objective:

To investigate effect of Shaoyaotang on intestinal mucosal immune barrier induced by 2

6-trinitrobenzene sulphonic acid (TNBS) in rats with ulcerative colitis.

Method:

Sixty SD rats were randomly divided into normal group

model group

mesalazine group (0.067 mg·kg

-1

)

low

medium and high-dose Shaoyaotang groups (1.8

3.6

7.2 g·kg

-1

). In the TNBS-induced ulcerative colitis model

saline

mesalazine

peony soup were administered by gavage for 7 days. Hematoxylin-eosin(HE) staining was used to detect the histopathological changes of colon tissue. The number of CD4

T lymphocytes and the expression of secretory immunoglobulin A(SIgA) in intestinal mucosa were detected by immunohistochemistry and Western blot.

Result:

Compared with normal group

the scores of intestinal mucosal injury and the pathological scores in model group increased significantly (

0.01). The expressions of CD4

T lymphocytes and SIgA in the intestinal mucosa of model group decreased significantly (

0.01). Compared with model group

the scores of colonic mucosal damage and the pathological scores decreased significantly in rats (

0.01). The expressions of CD4

T lymphocytes and SIgA in the intestinal mucosa of rats in each group elevated significantly (

0.05

0.01). Compared with mesalazine group and low-dose Shaoyaotang group

the intestinal mucosal injury score and the pathological scores in medium and high-dose Shaoyaotang groups decreased significantly (

0.01). The expression levels of CD4

T lymphocytes and SIgA protein in the intestinal mucosa of rats in middle and high doses Shaoyaotang groups increased significantly (

0.05).

Conclusion:

Shaoyaotang can reduce the intestinal mucosal damage and protect the intestinal mucosal immune barrier by increasing the number of CD4

T cells and the expression of SIgA secretion in the intestinal mucosa.

关键词

Keywords

references

王移飞，王凤仪，徐兰萍，等．芍药汤经HMGB 1 调节湿热型溃疡性结肠炎大鼠MyD88和NF- κ B的分子机制 [J]. 中国实验方剂学杂志， 2018 , 24 ( 12 ): 86 - 91 .

戴路明，朱磊，沈洪．基于 β 2 AR/ β -arrestin2/NF- κ B信号通路的清肠化湿颗粒防治溃疡性结肠炎的作用机制 [J]. 中国实验方剂学杂志， 2018 , 24 ( 9 ): 86 - 94 .

周文鹏，白爱平．炎症性肠病常见基因多态性 [J]. 胃肠病学， 2018 , 23 ( 3 ): 177 - 180 .

WANG K , WU L Y , DOU C Z , et al . Research advance in intestinal mucosal barrier and pathogenesis of crohn's disease [J]. Gastroenterol Res Pract , 2016 , doi: 10.1155/2016/9686238 http://doi.org/10.1155/2016/9686238 .

MA T M , XU N , MA X D , et al . Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats [J]. World J Gastroenterol , 2016 , 22 ( 8 ): 2566 - 2575 .

Vancamelbeke M , Vermeire S . The intestinal barrier: a fundamental role in health and disease [J]. Expert Rev Gastroenterol Hepatol , 2017 , 11 ( 9 ): 821 - 834 .

YU X T , XU Y F , HUANG Y F , et al . Berberrubine attenuates mucosal lesions and inflammation in dextran sodium sulfate-induced colitis in mice [J]. PLoS One , 2018 , doi: 10.1371/journal.pone.0194069 http://doi.org/10.1371/journal.pone.0194069 .

罗丹，仝战旗．中医药治疗溃疡性结肠炎的临床研究进展 [J]. 医学综述， 2017 , 23 ( 2 ): 336 - 339,343 .

齐雪阳，张勤生，张春燕．芍药汤加减保留灌肠联合美沙拉嗪肠溶片治疗大肠湿热证溃疡性结肠炎 [J]. 中国实验方剂学杂志， 2016 , 22 ( 19 ): 149 - 153 .

夏修文，陈桥桥，丁斗，等．芍药汤治疗溃疡性结肠炎的作用机制研究进展 [J]. 成都中医药大学学报， 2018 , 41 ( 3 ): 119 - 123 .

李冀，连建伟．方剂学 [M]．北京：中国中医药出版社， 2016 ： 89 - 90 .

LU Y , LIN H , ZHANG J , et al . Sijunzi Decoction attenuates 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats and ameliorates TNBS-induced claudin-2 damage via NF-κB pathway in Caco2 cells [J]. BMC Complement Altern Med , 2017 , 17 ( 1 ): 35 .

赵欣，恽海峰，向桂玲，等．白头翁汤加减联合双歧杆菌制剂对大鼠溃疡性结肠炎的影响 [J]. 中国实验方剂学杂志， 2018 , 24 ( 13 ): 110 - 116 .

贺石林，王键，王净净．中国科研设计与统计学 [M]．长沙：湖南科学技术出版社， 2013 ： 48 .

XU B , LIU X L , DONG W W , et al . EGCG maintains Th1/Th2 balance and mitigates ulcerative colitis induced by dextran sulfate sodium through TLR4/MyD88/NF- κ B signaling pathway in rats [J]. Can J Gastroenterol Hepatol , 2017 , doi: 10.1155/2017/3057268 http://doi.org/10.1155/2017/3057268 .

HOU Y , LU X , ZHANG Y . IRAK inhibitor protects the intestinal tract of necrotizing enterocolitis by inhibiting the Toll-like receptor (TLR) inflammatory signaling pathway in rats [J]. Med Sci Monit , 2018 , 24 : 3366 - 3373 .

孙健，高文艳，林一帆．溃疡性结肠炎病因和发病机制研究进展 [J]. 辽宁中医药大学学报， 2017 , 19 ( 4 ): 94 - 97 .

Rogler G , Andus T . Cytokines in inflammatory bowel disease [J]. World J Surg , 1998 , 22 ( 4 ): 382 - 389 .

YU W , LU B , ZHANG H , et al . Effects of the Sijunzi decoction on the immunological function in rats with dextran sulfate-induced ulcerative colitis [J]. Biomed Rep , 2016 , 5 ( 1 ): 83 - 86 .

SANG L X , CHANG B , ZHU J F , et al . Sodium selenite ameliorates dextran sulfate sodium-induced chronic colitis in mice by decreasing Th1, Th17, and γδ T and increasing CD4(＋) CD25(＋) regulatory T-cell responses [J]. World J Gastroenterol , 2017 , 23 ( 21 ): 3850 - 3863 .

CUI Y , ZHU C , MING Z , et al . Molecular mechanisms by which casein glycomacropeptide maintains internal homeostasis in mice with experimental ulcerative colitis [J]. PLoS One , 2017 , doi: 10.1371/journal.pone.0181075 http://doi.org/10.1371/journal.pone.0181075 .

王移飞，赵党生，王凤仪，等．芍药汤调节湿热型溃疡性结肠炎大鼠JAK2/STAT3和SOC3的分子机制 [J]. 中国实验方剂学杂志， 2017 , 23 ( 23 ): 97 - 102 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

芍药汤含药血清介导Fas/FasL信号通路抑制脂多糖诱导的Caco-2细胞炎症及凋亡

芍药汤通过调控糖代谢重编程影响Th17/Treg细胞平衡治疗溃疡性结肠炎

芍药汤调控巨噬细胞糖代谢重编程抑制M1型巨噬细胞极化

芍药汤通过SIRT6/HIF-1α途径抑制糖酵解治疗溃疡性结肠炎

基于网络药理学及实验验证探讨芍药汤治疗溃疡性结肠炎的作用机制