固本防哮饮对小鼠哮喘缓解期TLR4及TLR7的调节作用

卓月; 袁雪晶; 纪建建

doi:10.13422/j.cnki.syfjx.20190940

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固本防哮饮对小鼠哮喘缓解期TLR4及TLR7的调节作用

药理 | 更新时间：2021-02-09

- 固本防哮饮对小鼠哮喘缓解期TLR4及TLR7的调节作用
- Regulatory Effect of Guben Fangxiao Decoction on Expression of TLR4 and TLR7 in Mice with Asthma Remission
- 中国实验方剂学杂志 2019年25卷第10期页码：57-63
- 作者机构：
  
  南京中医药大学　附属医院，南京　 210029
- 作者简介：
  
  卓月，在读硕士，从事中医儿科学研究，E-mail：1954409441@qq.com
  *袁雪晶，博士，副教授，主任医师，硕士生导师，从事儿童哮喘、紫癜、抽动症等研究，E-mail：yuanxuejing2007@126.com
- 基金信息：
  
  江苏省“六大人才高峰”高层次人才B类资助项目(2016-WSN-059)
- DOI：10.13422/j.cnki.syfjx.20190940
  中图分类号： R289; R256.12; R563; R364.5; R285.5
- 收稿日期：2018-11-08，
  
  网络出版日期：2019-01-18，
  
  纸质出版日期：2019-05-20
- 稿件说明：
移动端阅览
卓月, 袁雪晶, 纪建建. 固本防哮饮对小鼠哮喘缓解期TLR4及TLR7的调节作用[J]. 中国实验方剂学杂志, 2019,25(10):57-63.

Yue ZHUO, Xue-jing YUAN, Jian-jian JI. Regulatory Effect of Guben Fangxiao Decoction on Expression of TLR4 and TLR7 in Mice with Asthma Remission[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 57-63.
卓月, 袁雪晶, 纪建建. 固本防哮饮对小鼠哮喘缓解期TLR4及TLR7的调节作用[J]. 中国实验方剂学杂志, 2019,25(10):57-63. DOI： 10.13422/j.cnki.syfjx.20190940.

Yue ZHUO, Xue-jing YUAN, Jian-jian JI. Regulatory Effect of Guben Fangxiao Decoction on Expression of TLR4 and TLR7 in Mice with Asthma Remission[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 57-63. DOI： 10.13422/j.cnki.syfjx.20190940.

摘要

目的：

观察固本防哮饮对哮喘缓解期小鼠模型Toll-样受体(TLR)4，TLR7，核转录因子-

B p65(NF-

B p65)，NF-

B抑制蛋白

)的调节作用，探讨固本防哮饮治疗哮喘缓解期的机制。

方法：

呼吸道合胞病毒(RSV)联合鸡卵蛋白(OVA)对3周龄BALB/c小鼠制备哮喘缓解期模型，将60只小鼠分为空白组、模型组、地塞米松组(0.001 g·kg

－1

)以及固本防哮饮低、中、高剂量(6.5，13，26 g·kg

－1

)组，分别进行干预，共给药28 d，每天1次。给药结束后处死小鼠，苏木素-伊红(HE)染色观察哮喘小鼠肺组织病理形态学变化，并对肺部炎症情况进行评分；蛋白免疫印迹法(Western blot)检测小鼠肺组织TLR4，TLR7，I

及肺组织细胞核内NF-

B p65蛋白表达水平；免疫荧光共聚焦观察NF-

B p65在细胞核表达情况。

结果：

与空白组比较，模型组小鼠肺组织有明显炎症细胞浸润及支气管狭窄(

0.01)，同时小鼠肺组织细胞核内NF-

B p65分布显著增多(

0.01)，表达明显增高(

0.05)，TLR4，TLR7，I

蛋白表达明显降低(

0.05)；与模型组比较，固本防哮饮各剂量组能显著缓解小鼠肺组织炎症浸润(

0.01)，降低肺组织核蛋白NF-

B p65的表达(

0.01)，核蛋白NF-

B p65入核情况减轻(

0.01)，同时显著增加TLR4，TLR7，I

蛋白的表达(

0.01)。

结论：

固本防哮饮能够缓解气道炎症，并且可能是通过调节TLR4，TLR7，NF-

B p65，I

实现其治疗作用。

Abstract

Objective:

To observe the regulatory effect of Guben Fangxiao decoction on Toll-like receptor (TLR)4

TLR7

nuclear factor-kappa B p65 (NF-

B p65) and NF-

B inhibitor protein alpha (I

) in mice with asthma remission

in order to explore the mechanism of Guben Fangxiao decoction in treating asthma remission.

Method:

Respiratory syncytial virus (RSV) combined with chicken ovalbumin (OVA) was used to build asthma remission model in 3-week-old BALB/c mice. Sixty mice were divided into blank group

model group

dexamethasone group (0.001 g·kg

-1

)

low

medium and high-dose Guben Fangxiao decoction group (6.5

26 g·kg

-1

)

respectively. Intervention was given once a day for 28 days. After administration

the mice were put to death. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of lung tissue and score the pulmonary inflammation. Western blot was used to detect the expressions of TLR4

TLR7

and nuclear NF-

B p65 in lung tissue of mice. Immunofluorescence was used to observe the expression of NF-

B p65 in cellnucleuses.

Result:

Compared with blank group

the lung tissue of model group showed obvious inflammatory cell infiltration (

0.01) and bronchial stenosis

the nuclear distribution of NF-

B p65 increased significantly (

0.01)

and the expressions of TLR4

TLR7 and I

proteins increased significantly (

0.05). Compared with the model group

Guben Fangxiao decoction could significantly alleviate the inflammatory infiltration of lung tissue in mice (

0.01)

reduce the expression of nuclear protein NF-

B p65 (

0.01)

relieve the nuclear entry of nuclear protein NF-

B p65 (

0.01)

and increase the expressions of TLR4

TLR7 and I

proteins (

0.01).

Conclusion:

Guben Fangxiao decoction can alleviate airway inflammation

and its therapeutic effect may be achieved by regulating TLR4

TLR7

NF-

B p65 and I

关键词

Keywords

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