Objective:

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To establish the HPLC fingerprint test method for the medicinal materials

decoction pieces and substance benchmarks of Shaoyao Gancaotang for investigating the quality transmitting of substance group in preparation process of the medicinal materials-decoction pieces-substance benchmarks

then to evaluate the scientificity and rationality of preparation process of substance benchmarks of Shaoyao Gancaotang by combining with yields of dry extract

transfer rates of effective components and other indexes.

Method:

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Substance benchmarks of Shaoyao Gancaotang was prepared according to the method recorded in ancient medical books

fingerprints of 15 batches of medicinal materials

decoction pieces and substance benchmarks were detected by HPLC

and the contents of effective ingredients were determined. At the same time

the correlation analysis of quality transmitting of substance group during the preparation of substance benchmarks was carried out by combining the yields of dry extract and transfer rates of effective components.

Result:

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The established HPLC fingerprint method has good precision

repeatability and stability

it can be used for the simultaneous determination of fingerprint of medicinal materials

decoction pieces and substance benchmarks. In the fingerprint of substance benchmarks

16 common peaks were determined by taking liquiritin as the reference peak

of which 6 chromatographic peaks belong to Paeoniae Radix Alba and 11 chromatographic peaks belong to Glycyrrhizae Radix et Rhizoma

7 major chromatographic peaks were identified. The similarities of fingerprints of 15 batches of medicinal materials

decoction pieces and substance benchmarks of Shaoyao Gancaotang were good by comparing with their respective reference fingerprints(≥0.90)

the average dry extract rate of 15 batches of substance benchmarks was 24.81%

and no discrete data were found; the average transfer rates of paeoniflorin

liquiritin and glycyrrhizic acid from decoction pieces to substance benchmarks were 79.68%

63.70% and 51.20%

respectively

and no discrete data were found.

Conclusion:

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In this paper

a scientific and reasonable method for evaluating the process of substance benchmarks of Shaoyao Gancaotang is established by means of the fingerprint method controlled by the whole substance group

the research idea of quality transmitting of substance group in the preparation process

and the evaluation of technical and economic indicators. It can be used as a reference for the evaluation and research of material benchmarks in other famous classical formulas.