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1.贵阳中医学院,贵阳 550002;
2.中国中医科学院 中药研究所,北京 100700
周雅阳,在读硕士,从事中药药理与毒理学研究,E-mail:375789730@qq.com
杜江,教授,从事苗医药理论与物质基础研究,E-mail:dujang.gz@163.com;
叶祖光,研究员,从事中药药理与毒理学研究,E-mail:yezuguang@ sina.com
收稿日期:2018-11-06,
网络出版日期:2019-01-17,
纸质出版日期:2019-06-20
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周雅阳, 张广平, 宋玲, 等. 痰热清吸入溶液的药效学评价[J]. 中国实验方剂学杂志, 2019,25(12):71-76.
Ya-yang ZHOU, Guang-ping ZHANG, Ling SONG, et al. Pharmacodynamic Evaluation of Tanreqing Inhalation Solution[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 71-76.
周雅阳, 张广平, 宋玲, 等. 痰热清吸入溶液的药效学评价[J]. 中国实验方剂学杂志, 2019,25(12):71-76. DOI: 10.13422/j.cnki.syfjx.20190954.
Ya-yang ZHOU, Guang-ping ZHANG, Ling SONG, et al. Pharmacodynamic Evaluation of Tanreqing Inhalation Solution[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 71-76. DOI: 10.13422/j.cnki.syfjx.20190954.
目的:
2
明确痰热清吸入溶液的镇咳、祛痰、解热及抗炎作用,为该制剂的进一步研发提供依据与数据支撑。
方法:
2
采用小鼠氨水引咳法及气管酚红排泌法分别观察痰热清吸入溶液的镇咳及祛痰作用;通过腹腔注射细菌脂多糖(LPS)建立大鼠发热模型,观察痰热清吸入溶液的解热作用;通过雾化吸入LPS建立大鼠急性肺炎模型,观察痰热清吸入溶液的抗炎作用。
结果:
2
痰热清吸入溶液可减少由氨水引发的小鼠咳嗽次数,增加小鼠气管酚红排泌量;降低LPS所致的大鼠体温及其相关调节因子前列腺素E
2
(PGE
2
)和环磷酸腺苷(cAMP)的水平;降低LPS所致大鼠急性肺炎支气管肺泡灌洗液(BALF)中白细胞(WBC)计数和中性粒细胞比例(NEUT),降低肺组织中核转录因子-
κ
B(NF-
κ
B)和白细胞介素-1
β
(IL-1
β
)的水平。
结论:
2
痰热清吸入溶液具有明显的镇咳、祛痰、解热及抗炎作用,值得进一步研发和推广。
Objective:
2
To clarify the antitussive
expectorant
antipyretic and anti-inflammatory effects of Tanreqing inhalation solution
and provide basis and data support for further research and development of this preparation.
Method:
2
The methods of cough induced by ammonia and tracheal phenol red excretion were used to observe the antitussive and expectorant effects of Tanreqing inhalation solution in mice. The fever model of rats was established by intraperitoneal injection of bacterial lipopolysaccharide(LPS) to observe the antipyretic effect of the Tanreqing inhalation solution
the acute pneumonia model of rats was established by atomizing LPS inhalation
and the anti-inflammatory effect of Tanreqing inhalation solution was observed.
Result:
2
Tanreqing inhalation solution could reduce the number of coughs in mice induced by ammonia water
increase the amount of phenol red excretion in mouse trachea
decrease the levels of body temperature and its related regulatory factors of prostaglandin E
2
(PGE
2
) and cyclic adenosine monophosphate(cAMP) of rats induced by LPS
decrease the white blood cell(WBC) count and the neutrophil ratio(NEUT) in bronchoalveolar lavage fluid(BALF) of rats with LPS-induced acute pneumonia
and reduce the levels of nuclear transcription factor-
κ
B(NF-
κ
B) and interleukin-1
β
(IL-1
β
) in lung tissue.
Conclusion:
2
Tanreqing inhalation solution has obvious antitussive
expectorant
antipyretic and anti-inflammatory effects
which is worthy of further development and promotion.
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