甘草附子汤对佐剂性关节炎小鼠滑膜成纤维样细胞增殖的影响

蔡悦; 张博; 郭静

doi:10.13422/j.cnki.syfjx.20191003

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甘草附子汤对佐剂性关节炎小鼠滑膜成纤维样细胞增殖的影响

经典名方 | 更新时间：2021-02-09

- 甘草附子汤对佐剂性关节炎小鼠滑膜成纤维样细胞增殖的影响
- Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model
- 中国实验方剂学杂志 2019年25卷第11期页码：29-33
- 作者机构：
  
  天津中医药大学　第一附属医院，天津　 300193
- 作者简介：
  
  蔡悦，硕士，主治医师，从事类风湿研究，E-mail：caiyue2018@126.com
- 基金信息：
  
  天津市科技计划项目(15ZXLCSY00020)
- DOI：10.13422/j.cnki.syfjx.20191003
  中图分类号： R2-0; R22; R285.5; R289
- 收稿日期：2018-10-01，
  
  网络出版日期：2019-02-12，
  
  纸质出版日期：2019-06-05
- 稿件说明：
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蔡悦, 张博, 郭静. 甘草附子汤对佐剂性关节炎小鼠滑膜成纤维样细胞增殖的影响[J]. 中国实验方剂学杂志, 2019,25(11):29-33.

Yue CAI, Bo ZHANG, Jing GUO. Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 29-33.
蔡悦, 张博, 郭静. 甘草附子汤对佐剂性关节炎小鼠滑膜成纤维样细胞增殖的影响[J]. 中国实验方剂学杂志, 2019,25(11):29-33. DOI： 10.13422/j.cnki.syfjx.20191003.

Yue CAI, Bo ZHANG, Jing GUO. Effect of Gancao Fuzitang on Synovial Fibroblast-like Cell Proliferation in Adjuvant Arthritis Mice Model[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 29-33. DOI： 10.13422/j.cnki.syfjx.20191003.

摘要

目的：

观察佐剂性关节炎(AA)小鼠滑膜组织成纤维样细胞(FLS)增殖的变化及甘草附子汤对其的影响。

方法：

将48只雄性Balb/c小鼠随机分为正常组，模型组，甘草附子汤组(7.8 g·kg

－1

)，雷公藤多苷组(10 mg·kg

－1

)。采用AA小鼠模型，模型成功后，治疗组小鼠给予相应给药灌胃18 d，1次/d，正常组和模型组小鼠灌胃等体积生理盐水。采用足肿胀法评价药物治疗作用；苏木素-伊红(HE)染色观察各组踝关节组织病理变化；Vimentin免疫组化法检测滑膜组织成纤维样增殖；免疫荧光法检测肿瘤坏死因子-

(TNF-

)表达水平以及蛋白免疫印迹法(Western blot)检测滑膜组织中细胞周期蛋白 D

(Cyclin D

)，增殖细胞核抗原(PCNA)，p53和p21蛋白表达。

结果：

与正常组比较，模型组足肿胀度增加，滑膜增生和骨侵蚀明显，滑膜组织成纤维样细胞增殖率升高(

0.05)，滑膜组织中TNF-

，Cyclin D

，PCNA蛋白表达明显升高(

0.05)，p53和p21蛋白表达降低(

0.05)。与模型组比较，甘草附子汤组小鼠足肿胀，滑膜增生和骨侵蚀有所改善，滑膜组织成纤维样细胞增殖率降低(

0.05)，滑膜组织中TNF-

水平，Cyclin D

，PCNA蛋白表达显著降低，而p53和p21蛋白表达升高(

0.05)。

结论：

甘草附子汤对AA小鼠关节和滑膜损伤具有明显改善作用，可能与降低局部慢性炎症，调节AA小鼠滑膜Cyclin D

，PCNA，p53和p21的蛋白异常表达，抑制FLS增殖有关。

Abstract

Objective:

To investigate the effect of Gancao Fuzitang on the proliferation of fibroblast-like synoviocytes (FLS) in synovial tissue of mice with adjuvant-induced arthritis (AA).

Method:

The 48 male Balb/c mice were randomly divided into 4 groups: normal control group

model group(arthritis induced through injection with adjuvant)

Gancao Fuzitang group and triptolide group. The normal control group and the model control group were orally given physiological saline. After the successful modeling

the mice of the other two groups were treated with Gancao Fuzitang or triptolide orally for 18 days. Then the animals were euthanized

the paw edema of the animals was measured

and arthritic ankles were observed by histological examination. Tumor necrosis factor (TNF)-

was examined by immunoassays. The proliferation of synovial cells of foot joints was detected by immunohistochemical expressions of Vimentin. The expressions of Cyclin D

proliferating cell nuclear antigen (PCNA)

p53 and p21 were detected by Western blot.

Result:

Compared with the normal control group

the mice of synovial hyperplasia and bone erosion in model group were obvious. The joint swelling degree

TNF-

level and proliferation of FLS were increased (

0.05). The expressions of Cyclin D

PCNA were up-regulated(

0.05)

but the expressions of p53 and p21 were down-regulated(

0.05). Compared with the model group

Gancao Fuzitang significantly alleviated joint swelling and injuries

reduced the level of TNF-

cell cycles (Cyclin D

and PCNA)

and increased protein expressions of p53 and p21 (

0.05).

Conclusion:

Gancao Fuzitang has a therapeutic effect on AA mice

and the mechanism might be associated with its anti-inflammatory effect and the effects in regulating Cyclin D

PCNA

p53 and p21 expressions and inhibiting FLS proliferation.

关键词

Keywords

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