黄芪多糖对高糖高脂饮食模型大鼠肠道甜味受体通路的影响

王颖; 陈思羽; 杨泽民

doi:10.13422/j.cnki.syfjx.20191037

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黄芪多糖对高糖高脂饮食模型大鼠肠道甜味受体通路的影响

药理 | 更新时间：2021-02-09

- 黄芪多糖对高糖高脂饮食模型大鼠肠道甜味受体通路的影响
- Effect of Astragalus Polysaccharide on Sweet Taste Receptor Pathway in Intestine of Rat Model Induced by High-sugar and High-fat Diet
- 中国实验方剂学杂志 2019年25卷第10期页码：64-68
- 作者机构：
  
  广东药科大学　生命科学与生物制药学院，广州　 510006
- 作者简介：
  
  王颖，在读硕士，从事中西医结合代谢性疾病机制研究和中药研发，E-mail：354695340@qq.com
  *杨泽民，博士，硕士生导师，高级实验师，从事中西医结合代谢性疾病机制研究和中药研发，E-mail：yzm3102001@gmail.com
- 基金信息：
  
  国家自然科学基金项目(81102703);广东省自然科学基金项目(2017A030313837);广东省科技计划项目(2013A032500005);广东药科大学自然科学培育项目(GYFYLH201303)
- DOI：10.13422/j.cnki.syfjx.20191037
  中图分类号： R285; R972+ .6; R587.1; R331.2+ 1
- 收稿日期：2018-12-01，
  
  网络出版日期：2019-02-19，
  
  纸质出版日期：2019-05-20
- 稿件说明：
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王颖, 陈思羽, 杨泽民. 黄芪多糖对高糖高脂饮食模型大鼠肠道甜味受体通路的影响[J]. 中国实验方剂学杂志, 2019,25(10):64-68.

Ying WANG, Si-yu CHEN, Ze-min YANG. Effect of Astragalus Polysaccharide on Sweet Taste Receptor Pathway in Intestine of Rat Model Induced by High-sugar and High-fat Diet[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 64-68.
王颖, 陈思羽, 杨泽民. 黄芪多糖对高糖高脂饮食模型大鼠肠道甜味受体通路的影响[J]. 中国实验方剂学杂志, 2019,25(10):64-68. DOI： 10.13422/j.cnki.syfjx.20191037.

Ying WANG, Si-yu CHEN, Ze-min YANG. Effect of Astragalus Polysaccharide on Sweet Taste Receptor Pathway in Intestine of Rat Model Induced by High-sugar and High-fat Diet[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 64-68. DOI： 10.13422/j.cnki.syfjx.20191037.

摘要

目的：

探讨黄芪多糖对高糖高脂饮食模型大鼠肠道甜味受体味觉受体第一家族成员2(T1R2)/味觉受体第一家族成员3(T1R3)通路的影响。

方法：

SD大鼠随机分为正常组、高糖高脂组和黄芪多糖组。高糖高脂饲料组和黄芪多糖组大鼠给予高糖高脂饲料喂养16周，期间黄芪多糖组大鼠每日给予0.7 g·kg

－1

的APS灌胃8周。采集大鼠血清测定空腹血糖，总胆固醇(TC)，甘油三酯(TG)，高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量；采集大鼠肠道组织，采用实时荧光定量聚合酶链式反应(Real-time PCR)检测甜味受体通路T1R2，T1R3，

-味导素(G

gust)和顺时受体电位阳离子通道5(TRPM5)以及胰高血糖素原(PG)mRNA的表达水平；蛋白免疫印迹法(Western blot)检测T1R2，G

gust和胰高血糖素样肽(GLP-1)蛋白表达水平。

结果：

与正常组比较，高糖高脂组大鼠血清TC，TG和LDL-C含量明显升高，HDL-C含量明显降低(

0.05)；肠道甜味受体通路中的T1R2，G

gust和PG mRNA表达水平明显降低(

0.05)；与高糖高脂组比较，黄芪多糖组大鼠血清TC，TG，LDL-C含量明显降低，HDL-C含量明显升高(

0.05)，肠道甜味受体通路分子T1R2，T1R3，G

gust，TRPM5以及PG mRNA表达水平明显升高(

0.05)，T1R2，G

gust和GLP-1蛋白表达明显升高；与模型组比较，黄芪多糖组T1R3 mRNA表达及T1R2，G

gust，GLP-1蛋白表达水平均明显降低(

0.05)。

结论：

黄芪多糖可改善高糖高脂饮食模型大鼠脂代谢紊乱和肠道甜味受体通路的损伤。

Abstract

Objective:

To observe the effect of astragalus polysaccharide (APS) on taste receptor 1 member 2 (T1R2)/taste receptor 1 member 3 (T1R3) sweet taste receptor pathway in intestine of rat model induced by high-sugar and high-fat diet.

Method:

SD rats were randomly divided into normal group

high-sugar and high-fat group and astragalus polysaccharide group. Rats in high-sugar and high-fat group and astragalus polysaccharide groups were fed with high-sugar and high-fat diet for 16 weeks

while rats in astragalus polysaccharide group were fed with APS (0.7 g·kg

-1

per day) for 8 weeks during this period. Serum samples were collected to determine the levels of fasting blood glucose

total cholesterol (TC)

triglyceride (TG)

high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). Intestinum tenue was collected to determine mRNA expressions of T1R2/T1R3

-gustducin (G

gust)

transient receptor potential cation channel subfamily member 5 (TRPM5) and proglucagon (PG) gene by Real-time PCR

and protein expressions of T1R2

gust and glucagon-like peptide-1 (GLP-1) protein by Western blot.

Result:

Rats in high-sugar and high-fat group had significantly higher levels of TC

TG and LDL-C

and lower HDL-C level in serum than those in normal group (

0.05). Moreover

the expression levels of sweet receptor pathway molecules

including T1R2

gust and PG genes in intestine

were significantly down-regulated in high-sugar and high-fat group (

0.05). Rats in astragalus polysaccharide group had significantly lower levels of TC

TG and LDL-C

and higher HDL-C level in serum than those in high-sugar and high-fat group (

0.05). The expression levels of T1R2

T1R3

gust

TRPM5 and PG genes in intestine were significantly up-regulated in astragalus polysaccharide group (

0.05). The trend of T1R2

gust and GLP-1 protein expressions was consistent with that of T1R2

gust and GLP-1 mRNA expressions. Protein expressions of T1R2

gust and GLP-1 and mRNA expression of T1R3 were significantly lower in astragalus polysaccharide group than those of control group (

0.05).

Conclusion:

APS could improve disturbance of lipid metabolism and impairment of intestinal sweet taste receptor pathway for rat model induced by high-sugar and high-fat diet.

关键词

Keywords

references

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