养精种玉汤治疗反复移植失败肾阴虚证的疗效及对LIF，VEGF，IL-1<italic style="font-style: italic">β</italic>，MCP-1的影响

张良; 张建伟; 吴海萃

doi:10.13422/j.cnki.syfjx.20191124

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养精种玉汤治疗反复移植失败肾阴虚证的疗效及对LIF，VEGF，IL-1β，MCP-1的影响

临床 | 更新时间：2021-02-09

- 养精种玉汤治疗反复移植失败肾阴虚证的疗效及对LIF，VEGF，IL-1β，MCP-1的影响
- Effect of Yangjing Zhongyu Tang in Treating Kidney-Yin Deficiency Due to Repeated Implantation Failure and on LIF, VEGF, IL-β, MCP-1 Levels
- 中国实验方剂学杂志 2019年25卷第11期页码：97-102
- 作者机构：
  
  1.山东中医药大学，济南　 250014；
  2.山东中医药大学　附属医院，济南　 250014
- 作者简介：
  
  张良，在读博士，主治医师，从事中医药在现代辅助生殖技术中应用研究，E-mail: 68669192@qq.com
  张建伟，博士，主任医师，从事中医辅助生殖技术研究，E-mail: zhangjianwei1970@sina.com
- 基金信息：
  
  国家自然科学基金青年基金项目(81703958);山东省中医药科技发展计划项目(2017-072;2017-067;2015-115)
- DOI：10.13422/j.cnki.syfjx.20191124
  中图分类号： R22; R242; R2-031; R287
- 收稿日期：2018-12-22，
  
  网络出版日期：2019-02-19，
  
  纸质出版日期：2019-06-05
- 稿件说明：
移动端阅览
张良, 张建伟, 吴海萃. 养精种玉汤治疗反复移植失败肾阴虚证的疗效及对LIF，VEGF，IL-1β，MCP-1的影响[J]. 中国实验方剂学杂志, 2019,25(11):97-102.

Liang ZHANG, Jian-wei ZHANG, Hai-cui WU. Effect of Yangjing Zhongyu Tang in Treating Kidney-Yin Deficiency Due to Repeated Implantation Failure and on LIF, VEGF, IL-β, MCP-1 Levels[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 97-102.
张良, 张建伟, 吴海萃. 养精种玉汤治疗反复移植失败肾阴虚证的疗效及对LIF，VEGF，IL-1β，MCP-1的影响[J]. 中国实验方剂学杂志, 2019,25(11):97-102. DOI： 10.13422/j.cnki.syfjx.20191124.

Liang ZHANG, Jian-wei ZHANG, Hai-cui WU. Effect of Yangjing Zhongyu Tang in Treating Kidney-Yin Deficiency Due to Repeated Implantation Failure and on LIF, VEGF, IL-β, MCP-1 Levels[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 97-102. DOI： 10.13422/j.cnki.syfjx.20191124.

摘要

目的：

探讨养精种玉汤治疗反复移植失败肾阴虚证的临床效果，探讨其机制。

方法：

选择山东中医药大学附属医院中西医结合生殖与遗传中心行体外受精-胚胎移植(IVF-ET)的反复种植失败(RIF)肾阴虚证患者70例，随机分为观察组、对照组，各35例。所有患者均行常规长方案进行超促排卵，观察组于移植前1个月的月经周期第5～14天服用养精种玉汤，并于移植当月月经周期第5天继续服用至移植日，对照组不服用中药。观察两组治疗后的中医证候积分；两组人体绒膜促性腺激素(HCG)日子宫内膜厚度、分型、子宫动脉搏动指数(PI)和阻力指数(RI)；子宫内膜分泌物白血病抑制因子(LIF)，血管内皮生长因子(VEGF)，白细胞介素-1

(IL-1

)和单核细胞趋化蛋白-1(MCP-1)；获卵数、受精率及胚胎移植结果。

结果：

治疗后，观察组中医证候积分较单纯西药组明显改善(

0.05)；治疗后两组子宫内膜厚度差异无统计学意义，A型内膜占比观察组明显高于对照组(

0.05)；PI两组间差异无统计学意义；RI观察组明显低于对照组(

0.05)；移植日观察组VEGF，IL-1

及LIF水平均高于对照组，MCP-1水平低于对照组(

0.05)；两组获卵数、受精率比较差异无统计学意义；观察组临床妊娠率明显高于对照组(

0.05)。

结论：

养精种玉汤可显著缓解RIF肾阴虚证患者的临床症状，有效提高了RIF患者A型子宫内膜形态，降低子宫动脉血流阻力，优化子宫内膜种植条件，显著提高临床妊娠率。

Abstract

Objective:

To explore the clinical efficacy of Yangjing Zhongyu Tang in treating kidney-Yin deficiency due to repeated implantation failure (RIF).

Method:

Totally 70 patients with kidney yin deficiency due to repeated implantation failure (RIF) who received the periodic Frozen thawed embyo (FET) after

in vitro

fertilization (IVF) embryo cryopreservation were selected from the Integrative Medicine Reproductive and Genetics Center of Affiliated Hospital of Shandong University of Traditional Chinese Medicine(TCM). They were randomly divided into observation group and control group

with 35 cases in each group. All patients were received long protocol controlled ovarian hyperstimulation. Observation group was given Yangjing Zhongyu Tang from the 5

day to the 14

day of the menstrual cycle 1 month before transplantation

and continued to take it on the 5

day of the menstrual cycle in the month of the transplantation. Control group did not given TCM. After treatment

TCM syndrome scores

endometrial thickness

typing

uterine artery pulsation index (PI) and resistance index (RI) of HCG

endometrial secretion leukemia inhibitory factor (LIF)

blood vessels endothelial growth factor (VEGF)

interleukin-1

(IL-1

) and monocyte chemoattractant protein-1 (MCP-1)

number of eggs obtained

fertilization rate and embryo transfer results were observed.

Result:

After treatment

the scores of TCM syndromes in observation group were significantly improved

with statistically significant difference (

0.05). There was no significant difference in endometrial thickness between two groups after treatment. The type of endometrium in observation group was significantly higher than that of control group (

0.05). There was no significant difference in PI between two groups. The RI value of observation group was significantly lower than that of control group (

0.05). VEGF

IL-1

and LIF of observation group were higher than those of control group

and the MCP-1 was lower than that of control group (

0.05). There was no significant difference in the number of eggs obtained and the fertilization rate between two groups. The clinical pregnancy rate of observation group was significantly higher than that of control group (

0.05).

Conclusion:

Yangjing Zhongyu Tang can significantly alleviate the clinical symptoms of patients with kidney-Yin deficiency due to RIF

effectively improve the type A endometrial morphology of RIF patients

reduce the uterine artery blood flow resistance

optimize endometrial implantation

and significantly improve the clinical pregnancy rate.

关键词

Keywords

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Effect of Yangjing Zhongyu Tang in Treating Kidney-Yin Deficiency Due to Repeated Implantation Failure and on LIF, VEGF, IL-β, MCP-1 Levels

DOI：10.13422/j.cnki.syfjx.20191124

摘要

Abstract

关键词

Keywords

references