Objective:

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To study HPLC fingerprints of Achyranthis Bidentatae Radix from different origins

compare different specifications in the same origin

and explore the effect of origin and specifications on the quality of Achyranthis Bidentatae Radix and relationship between the specifications and the internal quality of Achyranthis Bidentatae Radix

in order to provide basis for the identification of its origin.

Method:

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The HPLC fingerprints of Achyranthis Bidentatae Radix from different origins and with different specifications in the same origin were collected. The similarity analysis

cluster analysis and principal component analysis were adopted to analyze the fingerprints

the differences in fingerprints of Achyranthis Bidentatae Radix from different origins and with different specifications in the same origin were compared.

Result:

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Analysis of different origins and principal component analysis could be used to distinguish Achyranthis Bidentatae Radix from five producing areas

and the identification results of origin analysis was better than those of cluster analysis and similarity analysis. Analysis of different specifications

similarity analysis or principal component analysis could not distinguish Achyranthis Bidentatae Radix with different specifications.

Conclusion:

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There are significant differences in chemical composition and peak height among Achyranthis Bidentatae Radix from different origins

with less differences in chemical composition and peak height of Achyranthis Bidentatae Radix with different specifications

the principal component analysis could be used to identify origins of Achyranthis Bidentatae Radix.