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阿魏酸对H2O2致PC12细胞氧化损伤的保护作用
Protective Effect of Ferulic Acid on PC12 Cells with H2O2-induced Oxidative Damage
- 中国实验方剂学杂志 2019年25卷第13期 页码：66-72
- 作者机构：
广州中医药大学 中药学院，广州 510006
- 作者简介：
林秋珊，硕士，从事临床中药学研究，E-mail：13518816073@163.com
吴庆光，教授，主任医师，硕士生导师，从事临床中药学研究， E-mail：zyx321@gzucm.edu.cn
- 基金信息：国家自然科学基金项目(81673619)
DOI：10.13422/j.cnki.syfjx.20191202
中图分类号： R2-0; R22; R285.5收稿日期：2018-11-12，
网络出版日期：2019-03-01，
纸质出版日期：2019-07-05
- 稿件说明：
移动端阅览
林秋珊, 杨佩芬, 尹曼雪, 等. 阿魏酸对H2O2致PC12细胞氧化损伤的保护作用[J]. 中国实验方剂学杂志, 2019,25(13):66-72.
Qiu-shan LIN, Pei-fen YANG, Man-xue YIN, et al. Protective Effect of Ferulic Acid on PC12 Cells with H2O2-induced Oxidative Damage[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 66-72.
林秋珊, 杨佩芬, 尹曼雪, 等. 阿魏酸对H2O2致PC12细胞氧化损伤的保护作用[J]. 中国实验方剂学杂志, 2019,25(13):66-72. DOI： 10.13422/j.cnki.syfjx.20191202.
Qiu-shan LIN, Pei-fen YANG, Man-xue YIN, et al. Protective Effect of Ferulic Acid on PC12 Cells with H2O2-induced Oxidative Damage[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(13): 66-72. DOI： 10.13422/j.cnki.syfjx.20191202.
摘要
目的：
2
探讨阿魏酸(ferulic acid，FA)对H
2
O
2
诱导PC12细胞氧化损伤的保护作用及其作用机制。
方法：
2
H
2
O
2
诱导PC12细胞构建氧化应激损伤模型，不同剂量阿魏酸(终浓度为1，10，100 μmol·L
－1
)进行干预；采用噻唑蓝(MTT)比色法检测细胞存活率，生化法检测细胞上清液中乳酸脱氢酶(LDH)和丙二醛(MDA)的含量以及细胞中超氧化物歧化酶(SOD)的活性，采用实时荧光定量聚合酶链式反应(Real-time PCR)检测细胞中胰岛素样生长因子-1(IGF-1) mRNA的表达，蛋白免疫印迹法(Western blot)检测IGF-1蛋白的表达。
结果：
2
不同剂量的阿魏酸(终浓度为1，10，100 μmol·L
－1
)干预24 h后能明显改善H
2
O
2
诱导的PC12细胞的氧化损伤，与模型组比较，给药组细胞存活率明显升高，细胞上清液中LDH的漏出率明显减少(
P
<
0.01)；MDA的含量明显降低(
P
<
0.05)，同时细胞中SOD的含量显著升高(
P
<
0.01)；Real-time PCR结果显示，与空白组比较，模型组IGF-1 mRNA显著降低(
P
<
0.01)，与模型组比较，药物干预24 h后IGF-1 mRNA表达显著升高(
P
<
0.01)；Western blot结果显示，与空白组比较，模型组IGF-1蛋白的表达显著降低(
P
<
0.01)，与模型组比较，药物作用24 h后，能上调IGF-1蛋白的表达(
P
<
0.01)。
结论：
2
FA能够抑制H
2
O
2
诱导的PC12细胞的氧化损伤，其保护作用机制可能与胰岛信号通路相关。
Abstract
Objective:
2
To investigate the protective effect of ferulic acid on PC12 cells injured by H
2
O
2
and the molecular mechanisms.
Method:
2
The oxidative stress model was established by treating PC12 cells with H
2
O
2
and then different dosages of ferulic acid (1
10
100 μmol·L
-1
) were used for intervention. Methyl thiazolyl tetrazolium (MTT) assay was used to evaluate the cell viability
lactate dehydrogenase (LDH) and malondialdelyde (MDA) in cell supernatant
and superoxidedismutase (SOD) in cells was tested by biochemical method respectively. Insulin-like growth factors-1 (IGF-1) mRNA and protein expressions were detected by Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) and Western blot.
Result:
2
The 24 h intervention with different dosages of ferulic acid (1
10
100 μmol·L
-1
) could significantly improve the oxidative damage of PC12 cells induced by H
2
O
2
compared with the model group
ferulic acid at 1
10
100 μmol·L
-1
significantly increased PC12 cells viability
significantly decreased LDH and MDA content in cell supernatant (
P
<
0.05
P
<
0.01)
and enhanced SOD activity(
P
<
0.01). Real-time PCR and Western blot showed that compared with control group
IGF-1 mRNA and protein expressions in model group decreased significantly (
P
<
0.01)
compared with model group
IGF-1 mRNA and protein expressions in ferulic acid group increased significantly (
P
<
0.01).
Conclusion:
2
Ferulic acid exerts a protective effect on H
2
O
2
-inducing PC12 cells injury
which might be related to insulin signaling pathways.
关键词
Keywords
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