六味地黄丸基于SIRT6/NF-<italic style="font-style: italic">κ</italic>B信号通路对糖尿病肝损伤的保护作用

陆海英; 李志杰; 舒适; 张瑞义; 陶鹏宇; 张悦

doi:10.13422/j.cnki.syfjx.20191237

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六味地黄丸基于SIRT6/NF-κB信号通路对糖尿病肝损伤的保护作用

经典名方 | 更新时间：2021-02-09

- 六味地黄丸基于SIRT6/NF-κB信号通路对糖尿病肝损伤的保护作用
- Protective Effect of Liuwei Dihuangwan on Diabetes Mellitus with Liver Injury Based on SIRT6/NF-κB Signaling Pathway
- 中国实验方剂学杂志 2019年25卷第12期页码：28-34
- 作者机构：
  
  1.上海中医药大学，上海　 201203；
  2.上海市浦南医院，上海　 200125
- 作者简介：
  
  陆海英，在读博士，副教授，从事中医药防治慢性肝肾疾病基础研究，E-mail：haiying0411@qq.com
  张悦，博士，教授，博士生导师，从事中医药防治慢性肝肾疾病基础研究，E-mail：zhangyue-42@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81173406);上海中医药大学预算内项目(18LK023)
- DOI：10.13422/j.cnki.syfjx.20191237
  中图分类号： R289; R587.1; R575.5; R972+ .6; R285.5
- 收稿日期：2018-12-29，
  
  网络出版日期：2019-03-05，
  
  纸质出版日期：2019-06-20
- 稿件说明：
移动端阅览
陆海英, 李志杰, 舒适, 等. 六味地黄丸基于SIRT6/NF-κB信号通路对糖尿病肝损伤的保护作用[J]. 中国实验方剂学杂志, 2019,25(12):28-34.

Hai-ying LU, Zhi-jie LI, Shi SHU, et al. Protective Effect of Liuwei Dihuangwan on Diabetes Mellitus with Liver Injury Based on SIRT6/NF-κB Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 28-34.
陆海英, 李志杰, 舒适, 等. 六味地黄丸基于SIRT6/NF-κB信号通路对糖尿病肝损伤的保护作用[J]. 中国实验方剂学杂志, 2019,25(12):28-34. DOI： 10.13422/j.cnki.syfjx.20191237.

Hai-ying LU, Zhi-jie LI, Shi SHU, et al. Protective Effect of Liuwei Dihuangwan on Diabetes Mellitus with Liver Injury Based on SIRT6/NF-κB Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 28-34. DOI： 10.13422/j.cnki.syfjx.20191237.

摘要

目的：

从炎症角度探讨六味地黄丸对糖尿病伴肝损伤的保护机制。

方法：

选取自发性2型糖尿病动物模型db/db雄性小鼠18只，按空腹血糖(FBG)随机分模型组、六味地黄丸组(9.75 g·kg

－1

·d

－1

，1次/d)、白藜芦醇组(42.6 mg·kg

－1

·d

－1

，1次/d)；12只同窝野生型db/m小鼠，按FBG随机分正常组和六味地黄丸对照组(9.75 g·kg

－1

·d

－1

，1次/d)，正常组和模型组等量蒸馏水灌胃，各组给药16周后内眦静脉取血后处死小鼠，检测FBG，甘油三酯(TG)和丙氨酸氨基转移酶(ALT)等变化，苏木素-伊红(HE)染色观察肝组织病理改变，蛋白免疫印迹法(Western blot)检测肝组织沉默信息调节因子6(SIRT6)，核转录因子-

B p65(NF-

B p65)，单核细胞趋化蛋白-1(MCP-1)，血管细胞黏附因子-1(VCAM-1)和细胞间黏附因子-1(ICAM-1)蛋白表达。

结果：

与正常组比较，模型组小鼠FBG，TG和ALT显著升高(

0.01)，NF-

B p65，MCP-1，VCAM-1和ICAM-1蛋白表达显著升高(

0.01)，SIRT6蛋白表达显著降低(

0.01)；与模型组比较，六味地黄丸组和白藜芦醇组血FBG，TG显著降低(

0.01)，NF-

B p65，MCP-1和VCAM-1蛋白表达明显降低(

0.05，

0.01)，SIRT6蛋白表达明显升高(

0.05)，改善小鼠肝组织的肝细胞脂肪变性及炎细胞浸润。

结论：

六味地黄丸对糖尿病小鼠伴肝脏损伤有保护作用，其机制与调节血脂代谢，抑制炎症反应有关。

Abstract

Objective:

To investigate the protective mechanism of Liuwei Dihuangwan on diabetes mellitus with liver injury in terms of inflammation.

Method:

The 18 db/db mice were selected from animal model of spontaneous type 2 diabetes

mice were randomly divided into model group

Liuwei Dihuangwan group(9.75 g·kg

-1

·d

-1

once a day)

resveratrol group(42.6 mg·kg

-1

·d

-1

once a day)based on fasting blood-glucose (FBG). The 12 litter wild db/m mice were randomly divided into normal group

Liuwei Dihuangwan group(9.75 g·kg

-1

·d

-1

once a day). The normal group and the model group were given the same amount of distilled water by gavage. The mice in each group were treated for 16 weeks. FBG

triglyceride (TG) and alanine aminotransferase (ALT) were examined. Histopathological changes were observed by liver biopsy hematoxylin-eosin (HE) staining. Silent information regulator 6 (SIRT6)

nuclear factor-kappaB p65 (NF-

B p65)

monocyte chemoattractant protein-1 (MCP-1)

vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in the liver tissue were detected by Western blot.

Result:

Compared with normal group

FBG

TG and ALT in model group increased significantly(

0.01)

protein expression levels of NF-

B p65

MCP-1

VCAM-1 and ICAM-1 in model group were significantly up-regulated(

0.01)

the protein expression level of SIRT6 in model group was significantly down-regulated(

0.01). Compared with model group

Liuwei Dihuangwan and resveratrol could significantly reduce the levels of serum FBG and TG(

0.01)

the protein expression of SIRT6 was significantly increased(

0.01)

NF-

B p65

MCP-1 and VCAM-1 were significantly decreased (

0.05

0.01)

and liver steatosis and inflammatory cells infiltration were alleviated.

Conclusion:

Liuwei Dihuangwan can protect the diabetes mice from liver injury

and its mechanism is related to the improvement of lipid metabolism and the inhibition of inflammatory response.

关键词

Keywords

references

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Protective Effect of Liuwei Dihuangwan on Diabetes Mellitus with Liver Injury Based on SIRT6/NF-κB Signaling Pathway

DOI：10.13422/j.cnki.syfjx.20191237

摘要

Abstract

关键词

Keywords

references