Objective:

2

To measure the color values of Gentianae Radix et Rhizoma and the contents of gentiopicroside

the appearance index value of the representative color was correlated with the intrinsic index value representing the quality

in order to explore the correlation between the contents of active ingredients and the color values

and provide basis for the quality evaluation of Gentianae Radix et Rhizoma.

Method:

2

The color difference values of Gentianae Radix et Rhizoma powder was measured by colorimeter.The content determination method of gentiopicroside in the 2015 edition of

China Pharmacopoeia

was adopted. The content of gentiopicroside was determined by HPLC

and the correlation and regression analysis was carried out by SPSS 21.0 software.

Result:

2

There was a significantly positive correlation between the contents of gentiopicroside and

L

*

(representing colorshade)and

E

*

ab

(representing total color difference)(

P

<

0.01)

while the contents of gentiopicroside and

a

*

(representing color red-green direction) and

b

*

(representing color yellow-blue direction) had a significantly negative correlation (

P

<

0.01).

Conclusion:

2

The color value of Gentianae Radix et Rhizoma has a certain correlation with the content of gentiopicroside

and yellowish Gentianae Radix et Rhizoma contain more active ingredients

with a better quality.The contents of gentiopicroside active ingredients in Gentianae Radix et Rhizoma can be quickly predicted by determining the color difference values

which can provide a new idea for quality evaluation of this herb.