Objective:

2

To establish HPLC characteristic chromatogram of standard pieces of Polygoni Multiflori Radix

and compare with the HPLC characteristic chromatograms of original pieces

raw materials and control materials of Polygoni Multiflori Radix.

Method:

2

HPLC analysis was carried out on a Waters BEH-C

18

column (4.6 mm×250 mm

5 μm) with the mobile phase of methanol(A)-0.1%phosphoric acid(B) for gradient elution (0-20 min

15%-30%A; 20-35 min

30%-40%A; 35-55 min

40%-75%A; 55-75 min

75%-100%A) at a flow rate of 0.8 mL·min

-1

. The detection wavelength was set at 270 nm and the column temperature was maintained at 30℃. The injection volume was 10 μL. Moreover

similarity and cluster analysis of HPLC characteristic chromatograms of four samples of Polygoni Multiflori Radix were performed.

Result:

2

HPLC characteristic chromatogram of standard pieces of Polygoni Multiflori Radix composed of seven peaks was established. The similarity was 0.999 between characteristic chromatograms of standard pieces and original pieces

which was better than similarities among characteristic chromatograms of raw materials

control materials and original pieces. There was no obvious difference on number of peaks between characteristic chromatograms of standard pieces and original pieces

while an obvious difference on number of peaks between chromatograms of control materials and original pieces was found. In addition

standard pieces

original pieces and control materials could generally gather into one class

while raw materials could generally gather into another class.

Conclusion:

2

Compared with the raw materials and control materials

the established HPLC characteristic chromatogram of standard pieces can better reflect the internal quality of original pieces

which can be used for the quality control of decoction pieces of Polygoni Multiflori Radix.