多糖利用位点研究进展及其在中药药理研究中的应用

辜沅; 舒青龙

doi:10.13422/j.cnki.syfjx.20191505

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多糖利用位点研究进展及其在中药药理研究中的应用

综述 | 更新时间：2021-02-09

- 多糖利用位点研究进展及其在中药药理研究中的应用
- Research Progress of Polysaccharide Utilization Loci and Its Application in Pharmacological Research of Chinese Medicinal Herb
- 中国实验方剂学杂志 2019年25卷第16期页码：193-200
- 作者机构：
  
  江西中医药大学　基础医学院，南昌　 330004
- 作者简介：
  
  辜沅，在读硕士，从事中西医结合专业研究，Tel：0791-87118921，E-mail：2769264245@qq.com。
  舒青龙，博士，教授，硕士生导师，从事中医药微生态学研究，Tel：0791-87118921，E-mail：shuqinglong@126.com
- 基金信息：
  
  国家自然科学基金项目(31560038;81473455);江西省自然科学基金项目(20171BAB205087);江西省高等教育教学改革课题(JXJG-18-12-8)
- DOI：10.13422/j.cnki.syfjx.20191505
  中图分类号： R2-0; R22; R285.5; R284.1
- 收稿日期：2019-01-02，
  
  网络出版日期：2019-04-18，
  
  纸质出版日期：2019-08-20
- 稿件说明：
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辜沅, 舒青龙. 多糖利用位点研究进展及其在中药药理研究中的应用[J]. 中国实验方剂学杂志, 2019,25(16):193-200.

Yuan GU, Qing-long SHU. Research Progress of Polysaccharide Utilization Loci and Its Application in Pharmacological Research of Chinese Medicinal Herb[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 193-200.
辜沅, 舒青龙. 多糖利用位点研究进展及其在中药药理研究中的应用[J]. 中国实验方剂学杂志, 2019,25(16):193-200. DOI： 10.13422/j.cnki.syfjx.20191505.

Yuan GU, Qing-long SHU. Research Progress of Polysaccharide Utilization Loci and Its Application in Pharmacological Research of Chinese Medicinal Herb[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 193-200. DOI： 10.13422/j.cnki.syfjx.20191505.

摘要

多糖利用位点(PULs)是与多糖分解代谢相关且位于特定区域的一组基因簇，包括淀粉利用系统(SusC)，SusD，及编码外膜糖蛋白结合蛋白、编码碳水化合物活性酶等基因。多糖利用位点广泛存在于肠道拟杆菌中，这种能突出利用多糖的能力是拟杆菌适应肠道的生存策略之一。一方面，肠道拟杆菌的丰度高、种类繁多，是中药调节肠道菌群的最主要靶点菌群；另一方面，诸多中药化学成分中，中药多糖是重要有效成分且含量高，也可以作为肠道细菌的竞争性碳源；中药多糖如何基于碳源调节肠道拟杆菌是中药药理学研究的重要内容。本文结合最新的文献介绍了多糖利用位点的基因及蛋白构成、综述了多糖利用位点研究的最新进展，分析了多形拟杆菌和脆弱拟杆菌基因组中多糖利用位点多样性及其组成；同时，结合自己的中药多糖体外影响拟杆菌的碳源实验，对基于多糖利用位点研究中药微生态药理机制提出了展望，该方向的研究，不仅是细菌多糖利用位点研究的新内容，也是“人-药物-菌群”整体观及中医健脾研究的重要内容。

Abstract

Polysaccharide utilization loci (PULs) are a group of gene clusters related to polysaccharides catabolism and located in specific areas

including starch utilization system(SusC)

SusD

encoding outer membrane glycoprotein binding proteins

and carbohydrate active enzymes. The wide existence of PULs in Bacteroides

and the extraordinary ability of utilizing polysaccharide is its survival strategy to adapt to the intestinal tract. On one hand

Bacteroides feature a high abundance and variety

making it the most important bacterial target group regulated by traditional Chinese medicine(TCM). On the other hand

polysaccharide is an important effective constituent among TCM chemicals

with a high content

and can also be used as a competitive carbon source for intestinal bacteria. Therefore

what is the mechanism of regulating the intestinal flora based on the carbon source of polysaccharide is an important part of the pharmacological research of TCM. According to the latest literatures

this paper introduces the gene and protein composition of PULs

reviews the latest developments in PULs research

and analyzes the structure of PULs in the genomes of

Bacteroides fragilis

and

Bacteroides thetaiotaomicron

. Furthermore

we also put forward a prospect for the pharmacological micro-ecological mechanism of TCM based on PULs based on our carbon source experiments

which focuses the effects of Bacteroides by TCM polysaccharides

in vitro

. This research is not only the new content of bacterial PULs researches

but also the important part of researches of " human-drug-bacteria" holistic view and TCM spleen-tonifying concept.

关键词

Keywords

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从多糖利用位点的差异表达研究两种健脾中药复方多糖影响脆弱拟杆菌体外生长机制