左归丸防治骨质疏松症的动物模型和评价方法

刘飞祥; 林子璇; 徐道明; 叶素敏; 张明玥; 樊巧玲; 谭峰; 李星; 柴毅; 尚文斌; 卢圣峰

doi:10.13422/j.cnki.syfjx.20191525

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左归丸防治骨质疏松症的动物模型和评价方法

左归丸“补肾-益精-填髓-壮骨”效应的研究专题 | 更新时间：2021-02-09

- 左归丸防治骨质疏松症的动物模型和评价方法
- Review of Zuoguiwan on Animal Models and Evaluation in Prevention and Treatment of Osteoporosis
- 中国实验方剂学杂志 2019年25卷第18期页码：16-23
- 作者机构：
  
  1.南京中医药大学　基础医学院，第一临床医学院，国家重点省部共建针药结合实验室，南京　 210023；
  2.南京中医药大学　附属医院，南京　 210029
- 作者简介：
  
  刘飞祥，博士，从事中药复方的药效机制及配伍规律研究，E-mail：1353336582@qq.com
  樊巧玲，博士，教授，从事中药复方的药效机制及配伍规律研究，E-mail：fjxfql@163.com；
  谭峰，博士，讲师，从事中药复方的药效机制及配伍规律研究，E-mail：ver-1978@163.com
- 基金信息：
  
  国家自然科学基金项目(81573874;81873229);江苏省高等学校自然科学研究面上项目(18KJB360006);江苏省研究生科研创新计划项目(KYCX18_1525)
- DOI：10.13422/j.cnki.syfjx.20191525
  中图分类号： R22; R242; R2-031; R285.5
- 收稿日期：2019-01-18，
  
  网络出版日期：2019-04-18，
  
  纸质出版日期：2019-09-20
- 稿件说明：
移动端阅览
刘飞祥, 林子璇, 徐道明, 等. 左归丸防治骨质疏松症的动物模型和评价方法[J]. 中国实验方剂学杂志, 2019,25(18):16-23.

Fei-xiang LIU, Zi-xuan LIN, Dao-ming XU, et al. Review of Zuoguiwan on Animal Models and Evaluation in Prevention and Treatment of Osteoporosis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 16-23.
刘飞祥, 林子璇, 徐道明, 等. 左归丸防治骨质疏松症的动物模型和评价方法[J]. 中国实验方剂学杂志, 2019,25(18):16-23. DOI： 10.13422/j.cnki.syfjx.20191525.

Fei-xiang LIU, Zi-xuan LIN, Dao-ming XU, et al. Review of Zuoguiwan on Animal Models and Evaluation in Prevention and Treatment of Osteoporosis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 16-23. DOI： 10.13422/j.cnki.syfjx.20191525.

摘要

左归丸是滋阴补肾、填精益髓的经典名方，体现了“阳中求阴”的配伍思路，是防治骨质疏松症(OP)的常用有效方剂之一。OP动物模型模拟了OP在人体的病理状态和发病机制，是OP防治和新药研发的重要手段。该文详细论述了左归丸防治OP的2大类常用动物模型和特点，即原发性OP动物模型，包括去卵巢法动物模型和自发性老年OP动物模型；继发性OP动物模型，包括糖皮质激素诱导性动物模型，环磷酰胺诱导性动物模型以及肾大部切除性动物模型。左归丸防治OP的效用评价，包括酶联免疫吸附测定实验检测骨吸收标记物和骨形成标记物含量，双能X射线检测骨密度，显微CT分析骨小梁数量、骨小梁分离度、骨小梁厚度、骨体积/组织体积比以及骨表面/体积比，以衡量骨微结构水平，苏木精-伊红染色法观察骨病理形态，生物力学实验测试骨最大载荷力等生物力学特性。OP动物模型的成功与否，以及左归丸的药效作用，必须与5种不同的客观评价方法结合起来进行综合衡量，才能为左归丸的基础和临床研究提供科学的参考依据。但OP动物模型有待进一步优化，以凸显左归丸治疗OP的中医病机和证候特征。

Abstract

Zuoguiwan is a classic prescription for replenishing vital essence

tonifying kidney-Yin and nourishing the bone marrow. Zuoguiwan is one of the effective prescriptions for the prevention and treatment of osteoporosis (OP)

which reflects the thought of Reinforcing Yang from Yin. The OP animal model simulates the pathological state and pathogenesis of OP in human

which is an important means to research the pathogenesis of OP and verify the effect of drugs. In this paper

two kinds of animal models and characteristics of Zuoguiwan in the prevention and treatment of osteoporosis were discussed in details. They are the primary osteoporosis animal models

including ovariectomized animal models and spontaneous elderly osteoporosis animal models

and the secondary osteoporosis models

including glucocorticoid-induced animal models

cyclophosphamide-induced animal models and subtotal nephrectomy animal models. The evaluation methods of Zuoguiwan in preventing and treating OP

including bone absorption markers and bone formation markers analyzed by enzyme-linked immunosorbent assay (ELISA)

bone mineral density detected with dual-energy X-ray

the number of trabeculae

trabecular segregation

trabecular thickness

bone volume/tissue volume ratio and bone surface/volume ratio analyzed using micro-CT

bone pathological morphology observed by hematoxylin-eosin staining

bone biomechanical properties

such as the maximum load force based on biomechanical test. In order to provide scientific reference for the basic and clinical research of Zuoguiwan

the OP animal models and the pharmacodynamic effect of Zuoguiwan are evaluated comprehensively with five different and objective evaluation methods. However

the animal model of OP needs to be further optimized to highlight the pathogenesis and syndrome characteristics of Zuoguiwan in the treatment of OP.

关键词

Keywords

references

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