芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1，TNF-<italic style="font-style: italic">α</italic>表达的影响

王凤仪; 赵党生; 蒲晓薇; 祖健; 徐敏; 孙晨安

doi:10.13422/j.cnki.syfjx.20191603

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芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1，TNF-α表达的影响

经典名方 | 更新时间：2021-02-09

- 芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1，TNF-α表达的影响
- Effect of Shaoyaotang on Expressions of AP-1 and TNF-α in Colon of Rats with Hygrothermal Ulcerative Colitis
- 中国实验方剂学杂志 2019年25卷第16期页码：7-11
- 作者机构：
  
  甘肃中医药大学，兰州　 730000
- 作者简介：
  
  王凤仪，博士，硕士生导师，教授，从事中医四诊理论研究，E-mail：wfy@gszy.edu.cn
  赵党生，硕士生导师，教授，从事中医药治疗肛肠疾病研究，E-mail：zhds69@126.com
- 基金信息：
  
  国家自然科学基金项目(9984011510)
- DOI：10.13422/j.cnki.syfjx.20191603
  中图分类号： R2-0; R22; R285.5; R289
- 收稿日期：2018-12-03，
  
  网络出版日期：2019-05-08，
  
  纸质出版日期：2019-08-20
- 稿件说明：
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王凤仪, 赵党生, 蒲晓薇, 等. 芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1，TNF-α表达的影响[J]. 中国实验方剂学杂志, 2019,25(16):7-11.

Feng-yi WANG, Dang-sheng ZHAO, Xiao-wei PU, et al. Effect of Shaoyaotang on Expressions of AP-1 and TNF-α in Colon of Rats with Hygrothermal Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 7-11.
王凤仪, 赵党生, 蒲晓薇, 等. 芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1，TNF-α表达的影响[J]. 中国实验方剂学杂志, 2019,25(16):7-11. DOI： 10.13422/j.cnki.syfjx.20191603.

Feng-yi WANG, Dang-sheng ZHAO, Xiao-wei PU, et al. Effect of Shaoyaotang on Expressions of AP-1 and TNF-α in Colon of Rats with Hygrothermal Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(16): 7-11. DOI： 10.13422/j.cnki.syfjx.20191603.

摘要

目的：

观察芍药汤对湿热内蕴型溃疡性结肠炎(UC)大鼠模型结肠组织中激活蛋白-1(AP-1)与肿瘤坏死因子-

(TNF-

)的基因及蛋白表达的影响，探讨芍药汤治疗UC的作用机制。

方法：

将60只Wistar大鼠随机分为正常组、模型组、柳氮磺砒啶组及芍药汤低、中、高剂量组，采用病症结合的方法复制湿热内蕴型UC大鼠模型，即高脂高糖辛辣食物及免疫复合法，联合2，4，6-三硝基苯磺酸(TNBS)结合乙醇复合法。造模成功后，分别给予芍药汤低、中、高(6，12，24 g·kg

－1

)灌胃，柳氮磺胺嘧啶1 g·kg

－1

剂量灌胃，正常组给予等体积生理盐水，连续21 d。实时荧光定量聚合酶链式反应(Real-time PCR)检测结肠组织AP-1，TNF-

mRNA表达，蛋白免疫印迹法(Western blot)检测结肠组织AP-1，TNF-

蛋白表达。

结果：

与正常组比较，模型组AP-1，TNF-

mRNA及蛋白相对表达量明显升高(

0.05)；与模型组比较，各干预组AP-1，TNF-

mRNA及蛋白表达量明显下降(

0.05)，以芍药高剂量组较为明显。

结论：

芍药汤可抑制湿热型UC大鼠AP-1蛋白刺激TNF-

的表达，可能是其治疗湿热型UC的机制之一。

Abstract

Objective:

To observe the effect of Shaoyaotang on mRNA and protein expressions of colon tissue activated protein-1 (AP-1) and tumor necrosis factor-

(TNF-

) of hot and humid-type intrinsic ulcerative colitis (UC) model in rats

in order to explore the mechanism of action of herbaceous peony decoction in the treatment of UC.

Method:

Totally 60 Wistar rats were randomly divided into blank group

model group

SASP group

and low

medium and high-dose Shaoyaotang groups. The damp-heat intrinsic UC rat model was replicated based on integrated disease and syndrome

namely

high-fat and high-sugar spicy food and immune complex method combined with 2

6-trinitrobenzene sulfolnic acid (TNBS) and ethanol complex method. After the successful modeling

low

medium and high-dose Shaoyaotang (6

24 g·kg

-1

) was given by gavage

and 1 g·kg

-1

dose of salazol sulfadiazine was given to by gavage. The blank group was given constant volume normal saline for 21 d. Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) was used to detect mRNA expressions of AP-1 and TNF-

in colon tissues

and Western blot was used to detect protein expressions of AP-1 and TNF-

in colon tissues.

Result:

Compared with the blank group

relative mRNA and protein expressions of AP-1

TNF-

in the model group were significantly increased (

0.05). Compared with the model group

mRNA and protein expressions of AP-1

TNF-

in the treatment groups were significantly decreased (

0.05).

Conclusion:

Shaoyaotang can inhibit the expression of TNF-

and stimulate AP-1 protein expression in rats with damp-heat UC.

关键词

Keywords

references

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芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1，TNF-α表达的影响

Effect of Shaoyaotang on Expressions of AP-1 and TNF-α in Colon of Rats with Hygrothermal Ulcerative Colitis

DOI：10.13422/j.cnki.syfjx.20191603

摘要

Abstract

关键词

Keywords

references