Objective:

2

To established an approach of chemical fingerprinting and study the differences of the polysaccharides from three species of Polygonati Rhizoma in

Chinese Pharmacopoeia

so as to provide reference for quality evaluation and clinical application of Polygonati Rhizoma.

Method:

2

The polysaccharides were extracted by water extraction and alcohol precipitation from Polygonati Rhizoma. After hydrolysis by trifluoroacetic acid (TFA) and pre-column derivation by PMP

the chromatographic fingerprints of three kinds of Polygonati Rhizoma were established by high performance liquid chromatography. The fingerprinting model and chemometrics method

include similarity analysis (SA)

cluster analysis (HCA) and principal component analysis (PCA) were used for compare the differences among three species.

Result:

2

There were some differences in the PMP-HPLC fingerprints and monosaccharide composition from the three species. The

D

-mannose

L

-rhamnose and

L

-fucose were not detected

but they all contained

D

-galacturonic acid

D

-glucosamine hydrochloride

D

-galactose

D

-glucose and

D

-xylose among three species. The PCA and HCA analysis showed that chromatographic fingerprints of

P

.

cyrtonema

and

P

.

sibiricum

were similar

while

P

.

kingianum

and other two species were significantly different.

Conclusion:

2

There are differences in fingerprints of polysaccharides among three species of Polygonati Rhizoma. The possible effects of species should be considered in clinical application. The established PMP-HPLC is a simple

accurate and reproducible method

which can be used for the quality evaluation of Polygonati Rhizoma.