Objective:

2

Based on the analysis of the total components of fingerprint and the determination methods of the existing components in the 2015 edition of

Chinese Pharmacopoeia

the " point-line-surface" quality standard of Yangjing Zhongyu Tang was established by the " point" of each single component (morroniside

loganin

paeoniflorin

ferulic acid and verbascoside) to the " line" of multicomponent and the " face" of fingerprint of the whole component.

Method:

2

XB-C

18

column (4.6 mm×250 mm

5 μm) was used for gradient elution of 0.1%phosphoric acid aqueous solution-acetonitrile. The column temperature was 30 ℃

the injection volume was 10 μL

the flow rate was 1.0 mL·min

-1

and the detection wavelengths were 240

316

230

334 nm. The contents of these five components in Yangjing Zhongyu Tang were determined by three correction methods

external standard method and regression equation method. At the same time

the fingerprint of Yangjing Zhongyu Tang were analyzed by total component analysis and similarity evaluation.

Result:

2

With ferulic acid as reference

the relative correction factor (

f

) of morroniside

loganin

paeoniflorin and verbascoside were 0.392 1

0.421 4

0.261 7

0.268 6 by multi-point correction method

and their

f

(slope correction method) were 0.385 4

0.419 4

0.255 9

0.274 0

respectively. Twenty characteristic peaks of fingerprint were analyzed and the similarity was ≥0.999.There was no significant difference in the contents of these five components from Yangjing Zhongyu Tang determined by the quantitative assay of multi-components by single-marker (QAMS) correction method

the external standard method and the regression equation method.

Conclusion:

2

The comprehensive quality standard established by the total component analysis of fingerprint combined with various determination methods of existing components in the 2015 edition of

Chinese Pharmacopoeia

has been validated in famous classical formula of Yangjing Zhongyu Tang

which can provide ideas and methods for the quality control with quantitative determination and fingerprint of other famous classical formulas.